ABSTRACT
OBJECTIVE@#To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections.@*METHODS@#A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay.@*RESULTS@#The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection.@*CONCLUSIONS@#The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.
ABSTRACT
Objective: To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections. Methods: A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay. Results: The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection. Conclusions: The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.
ABSTRACT
An indirect ELISA test was optimised in which B meningoccocus outer membrane proteins included in the Cuban Vaccine known as VA-MENGOC-BC were used as capture antigents. Specific antibodies in serum samples from vaccinated individuals were detected using a human anti-IgG conjugate, i.e. alklayne phosphatase, thus the reaction developed with a specific substrate called p-nitrophenilphophate. Standard serum was obtained from a reference standard, the gage curve range was 625 to 20,000/mL. The control serum was selected from the most interesting areas for the samples, hence, assay characteristics were determined. Intra-assay, inter-assay and total inaccuracies were lower than 10 in the most linear area of the curve. Detectability was 700/mL. Recovery, paralelism and linearity studies showed an under 10 inaccuracy.