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OBJECTIVE@#To observe the efficacy of @*METHODS@#A total of 58 participants were included. Of them, 29 patients with insomnia were included into an observation group, and 29 healthy participants were included into a control group. The patients in the observation group were treated with @*RESULTS@#The total effective rate was 89.7% (26/29) in the observation group. In the observation group, the scores of PSQI, ISI and DISS, the A, B speed of NCT were all decreased after treatment (@*CONCLUSION@#The
Subject(s)
Humans , Acupuncture Therapy , Brain/diagnostic imaging , Emotions , Magnetic Resonance Imaging , Sleep Initiation and Maintenance Disorders/therapyABSTRACT
OBJECTIVE@#To observe the effect of electroacupuncture (EA) on neuronal apoptosis in rats with traumatic brain injury (TBI), and to explore the action mechanism of EA on improving the brain nerve function of TBI.@*METHODS@#A total of 88 6-week-old SD rats were randomly divided into a sham operation group, a model group, an EA group and a LY294002+EA group, 22 rats in each group. The TBI model on the left side was established by the improved Feeney's free fall method. After modeling for 24 h, the rats in the EA group and LY294002+EA group were treated with acupuncture at "Baihui" (GV 20) for 10 min and pricking acupuncture at "Shuigou" (GV 26) for 20 s; EA was applied at "Neiguan" (PC 6) and "Zusanli" (ST 36) on the right side (discontinuous wave, 2 Hz of frequency, 1 mA of intensity) for 10 min, once a day for 3 days. After 3 days of intervention, the TUNEL method was used to detect the level of neuron apoptosis in left cerebral cortex; the Western blot method was used to detect the expression of Akt, p-Akt, Bcl-2, Bax, Cyt-C and Caspase-9 in the left cerebral cortex.@*RESULTS@#After 3-day treatment, compared with the sham group, the number of neuronal apoptosis in the left cortex was increased in the model group (<0.01), and the expression of Bax, Cyt-C and Caspase-9 protein was increased (<0.01), and the expression of p-Akt/Akt, Bcl-2 was decreased (<0.01). Compared with the model group, the number of neuronal apoptosis in the left cortex was decreased in the EA group (<0.01), and the expression of Bax, Cyt-C and Caspase-9 was decreased (<0.01), and the expression of p-Akt/Akt and Bcl-2 was increased (<0.01). Compared with the LY294002+EA group, the number of neuronal apoptosis in the left cortex was decreased in the EA group (<0.01), and the expression of Bax, Caspase-9 and Cyt-C was decreased (<0.01, <0.05), and the expression of p-Akt/Akt and Bcl-2 was increased (<0.01).@*CONCLUSION@#EA could significantly reduce the neuronal apoptosis in rats with TBI, and its mechanism may be related to the activation of PI3K/Akt signaling pathway.
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Objective: To investigate the chemical constituents from the flesh of Trichosanthes kirilowii. Methods: The chemical constituents from n-butanol fraction of 95% ethanol extract of the flesh of T. kirilowii were separated by silica gel and ODS chromatogram columns as well as preparative HPLC. On the basis of NMR and MS data analysis, their structures were elucidated. Results: Twenty-six compounds were isolated from 95% ethanol extract of T. kirilowii, of which six organic esters were ethyl laurate (1), dibutyl phthalate (2), diethyl ethaneioate (3), dibutyl-2-malate (4), 6,10,14,18-tetramethyl-2-ethyl-7-ene-3-hydroxyl-ninecanol- 1-butyl ester (5), drechslerol-B (6), nine organic acids and phenolic acids were p-hydroxybenzaldehyde (7), salicylic acid (8), vanillic acid (9), isovanillic acid (10), protocatechuate (11), trans-cinnamic acid (12), p-hydroxycinnamic acid (13), trans-ferulic acid (14), and lauric acid (15), eight flavonoids were diosmetin (16), apigenin (17), chrysoeriol (18), luteolin (19), 4’-hydroxyscutellarin (20), quercetin (21), diosmetin-7-O-β-D-glucoside (22), chrysoeriol-7-O-β-D-glucoside (23), two aldehydes were 5-acetoxymethyl- 2-furaldehyde (24), 5-hydroxymethyl furfural (25) and one cycloaltinol compounds was cyclotucanol 3-palmitate (26). Conclusion: All compounds except compound 10 are isolated from the Trichosanthes genus for the first time.
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OBJECTIVE@#To investigate the effect of Health Qigong Baduanjin on the related indexes of obese middle aged women with diabetes and to provide new ideas for the intervention treatment of diabetes.@*METHODS@#A total of 40 middle-aged female obese diabetic patients were randomly divided into the control group and the exercise group(=20), the age was(57.2±5.4) years old. Fitness training group performed eight new Baduanjin exercises for 24 weeks of intervention, the control group did not exercise, body weight, waist circumference, body mass index (BMI), waist hip ratio (WHR), fasting blood glucose (FPG), glycosylated hemoglobin (HbAlc), triglyceride(TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) levels and serum retinol binding protein 4(RBP4) index were observed in the two groups.@*RESULTS@#After exercise, the waist, WHR, FPG, TG, HbAlc, HDL and RBP4 levels of the the patients in the experimental group were decreased significantly compared with those of before exercise and those of the patients in the experimental control group before and after exercise (<0.05).@*CONCLUSIONS@#Health Qigong Baduanjin can reduce the blood sugar of obese female patients with diabetes, and has some improvement effect on the body part of obesity and blood lipid indicators.