Comparative study on pharmacokinetics of senkyunolide I after administration of simple recipe and compound recipe in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a LC-MS method for the determination of senkyunolide I (SI) in rat plasma, in order to observe whether there is significant change in the pharmacokinetics parameters of complex prescriptions of Huoluoxiaolingdan (HLXL) and single herbal extracts from Ligusticum chuanxiong Hort. in rats, and assess the effect of other components in HLXL on the pharmacokinetics of SI.</p><p><b>METHOD</b>Twelve male Sprague-Dawley (SD) rats were randomly divided into two groups, and orally administered with extract from HLXL and L. chuanxiong (both equal to SI 4.53 mg x kg(-1)). Their blood was collected at different time points for LC-MS, in order to detect the plasma concentration of SI. The pharmacokinetic parameters of SI were calculated by DAS 2.0 software. SPSS 16.0 software was used for independent-sample T-test and Nonparametric T-test.</p><p><b>RESULT</b>A linear relationship of SI ranged from 6.750 to 675.0 microg x L(-1), and with the lowest limit of detection being 6.750 microg L(-1). Both of the plasma concentration-time curves of SI were fitted with the two-compartment model for extract of HLXL and L. chuanxiong. The detected AUC and Cmax of SI showed significant difference, with no significant difference in other parameters.</p><p><b>CONCLUSION</b>The LC-MS determination method established in this experiment was so exclusive, accurate and sensitive that it is suitable for pharmacokinetic studies on extracts of HLXL and SI from L. chuanxion. The experiment results show that other ingredients of HLXL have noticeable effect on the absorption of SI in rat plasma.</p>

Study on the compatibility of composite herbal medicines of the Zishen pill / 中国中药杂志

<p><b>OBJECTIVE</b>To study the compatibility of composite herbal medicines of the Zishen pill.</p><p><b>METHOD</b>50% ethanol extract test solution of the different combinations of composite herbal medicines of the Zishen pill were prepared. Pharmacologic experiments, such as anti-inflammatory, carbon particle clearance of RES were carried out with the solutions, and the corresponding pharmacological data were obtained. Variance analysis, canonical correlation and stepwise regression analysis were applied to interrelate the amount of each drug and the pharmacological data.</p><p><b>RESULT</b>The results confirmed that Huangbo and Zhimu were the basis, while Rougui was the corrigent, which was conformed to the theory of TCM.</p><p><b>CONCLUSION</b>This study provides a significant try for studying the compatibility of composite herbal medicines.</p>