ABSTRACT
Objective To observe the effect of electroacupuncture (EA) on the cognition and hippocampal tumor necrosis factor (TNF)-α and interleukin (IL)-1β expressions in rats with postoperative cognitive dysfunction (POCD), and discuss the action mechanism of EA.Method Ninety healthy male senile Sprague-Dawley (SD) rats were randomized into a sham operation group (group C), an operation group (group S) and an EA group (group Z), 30 rats in each group. Each group was then further divided into 3 subgroups: postoperative day 1, postoperative day 3 and postoperative day 7. The Morris water maze was adopted to evaluate the cognitive function, and immunochemistry method was used to detect the changes of TNF-α and IL-1β.Result Compared with group C at the same time points, the latent period was significantly longer and the crossing times were lower in group S on postoperative day 1, day 3 and day 7 (P<0.05), and the differences were most significant on postoperative day 3; compared with group S at the same time points, the latent period was significantly shorter and the crossing times were higher in group Z on postoperative day 1, day 3 and day 7 (P<0.05). Compared with group C at the same time point, the positive expressions of TNF-α and IL-1β were significantly higher in group S (P<0.05); compared with group S at the same time points, the positive expressions were significantly lower in each subgroup of group Z (P<0.05). Conclusion EA can improve the learning and memory of rats after left lateral segmentectomy of the liver, which is possibly related to the inhibition of the over-expressions of TNF-α and IL-1β.
ABSTRACT
Objective To observe the effect of electroacupuncture (EA) on the cognition and hippocampal tumor necrosis factor (TNF)-α and interleukin (IL)-1β expressions in rats with postoperative cognitive dysfunction (POCD), and discuss the action mechanism of EA.Method Ninety healthy male senile Sprague-Dawley (SD) rats were randomized into a sham operation group (group C), an operation group (group S) and an EA group (group Z), 30 rats in each group. Each group was then further divided into 3 subgroups: postoperative day 1, postoperative day 3 and postoperative day 7. The Morris water maze was adopted to evaluate the cognitive function, and immunochemistry method was used to detect the changes of TNF-α and IL-1β.Result Compared with group C at the same time points, the latent period was significantly longer and the crossing times were lower in group S on postoperative day 1, day 3 and day 7 (P<0.05), and the differences were most significant on postoperative day 3; compared with group S at the same time points, the latent period was significantly shorter and the crossing times were higher in group Z on postoperative day 1, day 3 and day 7 (P<0.05). Compared with group C at the same time point, the positive expressions of TNF-α and IL-1β were significantly higher in group S (P<0.05); compared with group S at the same time points, the positive expressions were significantly lower in each subgroup of group Z (P<0.05). Conclusion EA can improve the learning and memory of rats after left lateral segmentectomy of the liver, which is possibly related to the inhibition of the over-expressions of TNF-α and IL-1β.
ABSTRACT
In order to investigate the effect of Shouwu Shudi Yin on dopaminegic neurons in MPTP induced Parkinson's disease mouse model and the possible mechamism, the experimental mice were randomly divided into 4 groups: control, Shouwu Shudi Yin, MPTP and the treatment (MPTP+Shouwu Shudi Yin) groups. The number of tyrosine hydroxylase (TH) positive cells in the substantia nigra was measured by immunohistochemistry, and mRNA expression of TH and glutathione peroxidase (GPX) were detected by PCR. The results showed that the number of TH positive cells and mRNA expression of TH were significantly reduced in MPTP group compared with the control (P<0.05), and pretreated with Shouwu Shudi Yin didn't show protective effect. Compared to MPTP group, the mRNA expression of four subtypes of GPX were increased in various degrees in the treatment group pretreated with Shouwu Shudi Yin, although the difference was not statistically significant. These indicated that the preventive medication of Shouwu Shudi Yin don't have protective effect on the mice with Parkinson' s disease induced by MPTP, but it may enhance the antioxidant capacity through increasing the expression of GPX.