ABSTRACT
Aim To investigate the molecular mechanisms and inhibitory effect of honokiol (HNK) on the proliferation of colon cancer SW620 cells. Methods Crystal violet staining, flow cytometry and Western blot assays were used to for the detection of the effect of HNK on inhibiting the proliferation and promoting apoptosis on SW620 cells. Western blot was used to study the effect of HNK on the protein levels of TGF-β1 and analyze the effect of HNK on the phosphorylation of p38 MAPK, as well as the effect of HNK combined with exogenous adenoviruses of TGF-β1 and its inhibitor on the phosphorylation of p38 MAPK in SW620 cells. Western blot was also used to analyze the effect of HNK on the phosphorylation of YAP and analyze the possible relationship between HNK phosphorylation of p38 MAPK and YAP. Results HNK significantly inhibited the proliferation of SW620 cells and induced apoptosis, and up-regulated the expression levels of TGF-β1. Western blotting showed that HNK up-regu- lated the expression levels of phosphorylated p38 in SW620 cells. Meanwhile, HNK increased the protein levels of phosphorylated YAP. The exogenous adenoviruses of TGF-β1 and its inhibitor significantly enhanced or inhibited this effect, respectively. Conclusions HNK has obvious antiproliferative effect, which might be due to the up-regulation of TGF- (31 expression and up-regulation of phosphorylated YAP expression by activating the p38 MAPK signaling pathway.
ABSTRACT
Aim To investigate the anti-proliferation effect of honokial on human colon cancer cells HCT116 and the possible mechanism. Methods Crystal stai-ning and flow cytometry assay were introduced to test the proliferation inhibitory and cell cycle arrested effect of honokial on HCT116 cells. Western blot was used to analyse the expression of PCNA and induction effect of apoptosis. Western blot and PCR assay were conducted to assess the change of BMP7. Via exogenous adenovi-ruses of BMP7 and its antibody combined with honoki-al,crystal violet staining and Western blot were used to analyse the effect on HCT116 cells. Results Honoki-al inhibited the growth of HCT116 in a time-and con- centration-dependent way,induced apoptosis and arres-ted at G2 phase; Western blot assay showed honokial up-regulated the level of PCNA and BMP7,and down-regulated the expression of Bcl-2; Western blot result also showed that exogenous adenoviruses of BMP7 en-hanced the effect of honokial on proliferation inhibition and apoptosis, while BMP7 antibody reversed such effects of honokial. Conclusion Honokial can inhibit the proliferation and induce apoptosis on HCT116 cells,which may be mediated by the up-regulation of BMP7.