Analysis on Scientific Projects about TCM Constitutions Funded by National Natural Science Foundation of China / 中国中医药信息杂志

Objective To analyze scientific projects about TCM constitutions funded by National Natural Science Foundation (NSFC); To understand its development trend and current situation.Methods The scientific projects about TCM constitutions in the fields of H27 and H29 were retrieved in NSFC Network Information System during 1996-2016 and "Dingxiangtong" during 1985-2015. Project year, branch discipline, project category, relying-on units were analyzed. PICO model of evidence-based medicine to build clinical problems was applied to analyze project research objects, clinical or scientific issues, interventions, methods, effects indicators, and the role of links. Results There were totally 61 scientific projects and 26.38 millions yuan funded by NSFC in TCM constitutions during 1988-2016. Project and the amount of fund to overall were on the rise, especially since 2010. There were 48 projects about TCM science, 21.77 million yuan (82.5％), 13 projects about integrated traditional Chinese and Western medicine, 4.61 million yuan (17.5％), including 27 surface projects, 11.32 million yuan (42.9％), 17 youth science fund, 3.32 million yuan (12.6％), 15 regional science fund projects, 6.34 million yuan (24.0％), and 2 key projects, 5.4 million yuan (20.5％). The units with the most foundation were Beijing University of Chinese Medicine (15), Xinjiang Medical University (6), Shanghai University of Traditional Chinese Medicine (5). TCM constitution of zang-fu, qi and blood, and body fluid, clinical basis of integrated traditional Chinese and Western medicine, and basic principles of integrated traditional Chinese and Western medicine were the branches with the most foundation. Conclusion s Supported by NSFC, marked improvement and progress have been achieved in research on TCM constitutions.

Development of Multiplex Immunoassay Based on Inductively Coupled Plasma Mass Spectrometry / 分析化学

Immunoassay is of great importance in the field of clinical detection. Our group proposes the inductively coupled plasma mass spectrometry ( ICP-MS) based immunoassay. Multiplex immunoassay can be realized by stable isotope tagging strategy combined with ICP-MS detection. Since then, large amount of researches in this field have been made at home and abroad, and the method has been proved applicable to the analysis of biological small molecules, proteins, nucleic acids and cells. In this article, the history and development of immunoassay, the characters of ICP-MS based immunoassay, single component and multiplex immunoassay, and the magnetic immunoassay that is recently fast developed are reviewed. The prospect of the developing tendency is also anticipated, hoping to provide references for the research in this field.

Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate activation of brown adipose tissue (BAT) stimulated by medium-chain triglyceride (MCT).</p><p><b>METHODS</b>30 Male C57BL/6J obese mice induced by fed high fat diet (HFD) were divided into 2 groups, and fed another HFD with 2% MCT or long-chain triglyceride (LCT) respectively for 12 weeks. Body weight, blood biochemical variables, interscapular brown fat tissue (IBAT) mass, expressions of mRNA and protein of beta 3-adrenergic receptors (β3-AR), uncoupling protein-1 (UCP1), hormone sensitive lipase (HSL), protein kinase A (PKA), and adipose triglyceride lipase (ATGL) in IBAT were measured.</p><p><b>RESULTS</b>Significant decrease in body weight and body fat mass was observed in MCT group as compared with LCT group (P<0.05) after 12 weeks. Greater increases in IBAT mass was observed in MCT group than in LCT group (P<0.05). Blood TG, TC, LDL-C in MCT group were decreased significantly, meanwhile blood HDL-C, ratio of HDL-C/LDL-C and norepinephrine were increased markedly. Expressions of mRNA and protein of β3-AR, UCP1, PKA, HSL, ATGL in BAT were greater in MCT group than in LCT group (P<0.05).</p><p><b>CONCLUSION</b>Our results suggest that MCT stimulated the activation of BAT, possible via norepinephrine pathway, which might partially contribute to reduction of the body fat mass in obese mice fed high fat diet.</p>

Study on associated biomarkers influencing recurrence, metastasis and prognosis in patients with gastrointestinal stromal tumors after complete resection / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To explore the associated biomarkers influencing recurrence, metastasis and prognosis in patients with gastrointestinal stromal tumors (GIST) after complete resection.</p><p><b>METHODS</b>Tumor tissue samples of 148 patients with GIST undergoing complete resection from January 1990 to December 2008 in Sun Yat-sen University Cancer Center were collected. The expressions of Ki-67, E-cadherin, MMP7, CD44, nm23, P53, survivin, Cyclin D1, COX-2, and VEGF in tumor tissue samples were detected by tissue microarray and immunohistochemistry (IHC). The association of above factors expressions with recurrence, metastasis and prognosis was examined.</p><p><b>RESULTS</b>Log-rank test showed that Ki-67, E-cadherin, MMP7, CD44, P53 and survivin were associated to disease-free duration after complete GIST resection (all P<0.05), and the Ki-67, E-cadherin, P53 and survivin were associated to overall survival (all P<0.05). Cox multivariate analysis revealed that disease-free survival was associated with Ki-67, CD44 and P53 (all P<0.05), and the overall survival was only associated with Ki-67 (P<0.05).</p><p><b>CONCLUSION</b>Ki-67, CD44 and P53 are closely associated with recurrence and metastasis after complete GIST resection, and Ki-67 can predict the prognosis of GIST.</p>

Medium- and long-chain fatty acid triacylglycerol reduce body fat and serum triglyceride in overweight and hypertriglyceridemic subjects / 中华预防医学杂志

<p><b>OBJECTIVE</b>To investigate effects of medium- and long-chain fatty acid triacylglycerols (MLCT) on body fat and serum lipid in overweight and hypertriglyceridemic subjects.</p><p><b>METHODS</b>A double-blind, controlled clinical trial was carried out, in which 112 subjects with hypertriglyceridemia were enrolled and divided into two groups, there were 56 subjects in each group. One group was randomized to consume long-chain fatty acid triacylglycerol (LCT), and the other to MLCT. All volunteers were asked to consume 25 - 30 g test oil daily for consecutive 8 weeks. Anthropometric measurements of body weight, body fat weight, waist circumference(WC), hip circumference(HC), WHR (ratio of WC/HC), total fat weight, subcutaneous fat area, visceral fat area, and serum biochemical variables of glucose, total cholesterols(TC), triglycerides(TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C)were measured at the initial and final time of the study.</p><p><b>RESULTS</b>11 subjects were excluded from the study because of various reasons. Of the 101 included cases, there were 50 (male subject 34, 68.0%) and 51 (male subject 33, 64.7%) subjects left in LCT and MLCT group respectively. The proportion of men in MLCT (64.7%, 33/51) was not significantly different (chi(2) = 0.1227, P > 0.05) compared to those in LCT (68.0%, 34/50). The average age of MLCT was (54.2 +/- 12.5) which was not significantly different (t = 0.39, P > 0.05) compared to those in LCT (53.2 +/- 13.0); Body mass index (BMI) of MLCT was (25.9 +/- 3.3) kg/m(2), which was not significantly different (t = 0.08, P > 0.05) compared to those of LCT (25.9 +/- 2.4) kg/m(2). After consumption of test oil for 8 weeks, extent of decrease in BMI, percent of body fat, subcutaneous fat, serum TG and serum LDL-C in overweight subjects of MLCT were (-0.73 +/- 0.61) kg/m(2), (-1.53 +/- 1.32)%, (-16.29 +/- 19.25) cm(2), (-0.57 +/- 0.86) mmol/L and (-0.05 +/- 0.64) mmol/L respectively, those in overweight subjects of LCT were (-0.19 +/- 0.61) kg/m(2), (-0.58 +/- 1.02)%, (4.69 +/- 19.06) cm(2), (0.65 +/- 1.10) mmol/L and (0.38 +/- 0.58) mmol/L respectively, all of them were significantly different (the value of t were -2.70, -2.43, -3.20, -3.81 and -2.09 respectively, all of P value were less than 0.05).</p><p><b>CONCLUSION</b>Consumption of MLCT can reduce body fat weight and serum triacylglycerol and LDL-C in overweight hypertriglyceridemic subjects under an appropriate dietary regime.</p>

Effects of Medium-and Long-chain Fatty Acid Triacylglycerol on Lipid Metabolism in Different Age's Male Subjects with Hypertriglyceridemia / 中国慢性病预防与控制

Objective To investigate the effects of medium-and long-chain fatty acid triacylglycerols on lipid metabolism in different age's male subjects with hypertriglyceridemia.Methods A randomized double-blind and controlled clinical trial was carried out among 74 male cases with hypertriacylglycerolemia.They were divided into two groups,one group was randomized to taking long-chain fatty acid triacylglycerol oil(LCT),and the other one to medium-and long-chain fatty acid triacylglycerol oil(MLCT).All cases in two groups asked to consume 20～30 g oil daily.Anthrepometric measurements and blood biochemical variables were measured at the initial and final time of the study.Results There were 34 and 33 cases left in LCT and MLCT group,respectively.A greater extent of decrease in the levels of blood TG,TC,apoB,apoA Ⅰ,apoAⅡ,apoC Ⅱ,apoC Ⅲ,apoE and LDL-C in male subjects aged less than 60 years was found in MLCT group than in LCT group after 8 weeks study(P