ABSTRACT
OBJECTIVE@#Research on the application feasibility of SNP genotyping for forensic identification by microarrays.@*METHODS@#Oligonucleotide microarrays which could detect 31 different SNPs were used. Population studies on 31 SNP loci was carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases.@*RESULTS@#No deviations from Hardy-Weinberg equilibrium could be found at the 31 SNP loci 4 loci were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal informative SNPs was 0.9999999999979. The matching probability was 2.13 x 10(-12). The average exclusion probability in duos and trios were 0.9609 and 0.9970 respectively.@*CONCLUSION@#The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification.
Subject(s)
Humans , Asian People/genetics , DNA Fingerprinting/methods , DNA Primers , Forensic Medicine/methods , Genotype , Oligonucleotide Array Sequence Analysis , Paternity , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Tandem Repeat SequencesABSTRACT
OBJECTIVE@#ABO genotyping for forensic identification by oligonucleotide chip.@*METHODS@#Oligonucleotide microarrays which could detect 3 different SNPs in exon 6 and exon 7 for ABO genotyping were used. Population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals. The method was also applied to cases.@*RESULTS@#The technique could identify 6 genotypes of ABO system. According to the results of population studies, no significant deviations from Hardy-Weinberg equilibrium could be found. The observed and expected heterozygosities were 0.591 and 0.616 respectively. The polymorphic information content was 0.544. The average exclusion probabilities in buos and trios was 0.188 and 0.344 respectively. The discrimination power is 0.777.@*CONCLUSION@#The data and case application demonstrated that ABO typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification.