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Objective To observe the effect on succinate dehydrogenase (SDH) of mitochondria in myocardium and liver in sepsis rats treated with edaravone. Methods 30 Sprague-Dawley rats were divided into 3 groups: sham operated group ( group A ), controlled operated group ( group B ), treated group with edaravone (group C). The model of sepsis rats was made by the way of caecum ligated and punctured and 20mg/kg lactate levofloxacin was subcutaneously injected (sci) 15min before and 3h after operation in three group. 5mg/kg edaravone were sci 15min before and 3h after operation in group C. Liver and myocardium were taken from all of them 18h after operation. The activities of SDH in myocardial and hepatic mitochondria were detected, pathological change of mitochondria in liver and myocardium were observed. Results The activities of SDH in myocardial and hepatic mitochondria in group B [ (0. 21 ± 0. 07 ) U/mgprot, (0. 23± 0. 08 ) U/mgprot ] were significantly decreased compared with group A [ ( 0. 33 ± 0. 10 ) U/mgprot, ( 0. 38±0. 12)U/mgprot]. The activities of those in group C[ (0.31 ±0. 08) U/mgprot, (0. 36 ±0. 11)U/mgprot] were significantly increased than group B. Myocardial and hepatic mitochondria swelling and endocytoplasmic reticulum expanding were found in group B by electron microscope, while it showed normal in group C. Conclusion Hepatic and myocardial mitochondrial structure were destroyed and activities of SDH were decreased in sepsis rats. They could be effectively protected by edaravone.
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Objective To evaluate the diagnostic value of serum neuron specific enolase(NSE) dynamic changes in brain death patients.Methods After onset of brain death,the serum NSE levels at 24,48 and 72 hours were determined by enzyme linked immunosorbent assay(ELISA) in 30 such patients(brain death group),and the NSE levels were also determined by the same method in 30 extra severe craniocerebral injury patients(cerebral injury group)as concurrent control for comparisons.Results The serum NSE level increased over time both in brain death and severe craniocerebral injury patients.The serum NSE levels of brain death patients were higher than those in extra severe craniocerebral injury patients significantly at 24,48 and 72 hours after onset respectively(all P370 ?g/L) in 5 days,and 3 cases in 7 days.But similar phenomenon was not observed in 13 cases with severe craniocerebral injury and having stayed in ICU over 1 week whose serum NSE level,on the contrary,was decreased gradually after 3 days of onset. It was statistical significant when the serum NSE levels were compared at the same time points between the two groups(P
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0.05).CD3~+,CD4~+,CD4~+/ CD8~+ in A group on the 8th day were higher(P0.05) than those on the 1st day.Compared to B group,CD3~+,CD4~+,CD4~+/ CD8~+ in A group on the 8th day were increased significantly(P
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OBJECTIVE To determine the sensitivity of Vibrio vulnificus to 23 kinds of antibacterial drugs,and offer the evidence for clinical V.vulnificus infections treatment.METHODS Isolated 11 strains of V.vulnificus from the patients blood and blisters of infected parts,using ATB automatic identification and susceptibility testing to measure the vitro drug sensitivity of the bacteria.RESULTS The susceptibility of the bacteria to the 22 kinds of commonly used antibiotics showed 100% sensitivity,the rate of ciprofloxacin sensitivity was 72%.CONCLUSIONS It is sensitive to commonly used semi-synthetic penicillin,cephalomycin,carbapenem,aminoglycosides,the third generation cephalosporins,fluoroquinolones and so on,all these above drugs may be chosen to treat the V.vulnificus infection.
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Objective To observe noninvasive bi-level positive pressure ventilation for the treatment of severe acute left heart failure.Methods 16 patients with hypoxemia due to severe acute heart failure with non-reaction to the normal treatment received noninvasive bi-level positive pressure ventilation and were monitored under clinical symptoms,signs,traumatic arterial blood pressure and blood gas analysis.Results 16 patients survived and 1 died with success rate of 93.7%.After two-hour therapy,the patients vital signs were stable and PaO2 and SaO2 went up.Conclusion Noninvasive bi-level positive pressure ventilation can decrease the pulmonary interstitial and alveolar edema,improve left heart function through adjusting pressure support and end-respiratory positive pressure.It also improve the rate of V/Q,facilitate gas exchange,and promote PaO2 and SaO2.