ABSTRACT
Objective To label granulocytes in a state of differentiation in mouse bone marrow (BM) with EdU (5-ethynyl-2′-deoxyuridine) for further understanding the changes in granulocyte produc-tion at different stages of differentiation during inflammation. Methods C57BL/6 mice were intraperitoneal-ly (i.p.) injected with EdU and heat-inactivated Escherichia coli(HI E.coli). BM cells were harvested at different time points after HI E.coli injection and then stained with fluorescent-conjugated antibodies(Abs). Myeloblasts,promyelocytes,myelocytes, metamyelocytes and band and segmented neutrophils were identi-fied by fluorescence-activated cell sorting(FACS). The percentage of EdU-positive cells in each population was recorded. Results The percentage of EdU-positive myeloblasts in mice increased by 10.0% at 24 h af-ter intraperitoneal injection with HI E.coli,but decreased by 75.0% and 23.0% at 48 h and 72 h,respec-tively. The percentage of EdU-positive promyelocytes declined by 23.0%,54.5%,64.3% and 77.8% at 24 h,48 h,72 h and 96 h,respectively. The percentage of EdU-positive myelocytes increased by 60.0% and 10.0% at 24 h and 48 h,but decreased by 80.0% and 90.0% at 72 h and 96 h. The percentage of EdU-positive metamyelocytes increased by 50.0% at 24 h,but decreased by 33.3%,61.5% and 66.7% at 48 h,72 h and 96 h. The percentage of EdU-positive band and segmented cells increased by 14.0% at 24 h,but decreased by 50.0%, 77.8% and 88.0% at 48 h, 72 h and 96 h. Conclusion Emergency granulopoiesis occurred 24 h after the establishment of HI E.coli-induced model of acute peritonitis, which meant that the proliferation of myeloid precursor cells,especially that of myelocytes and metamyelocytes,was accelerated and resulted in increasing number of mature neutrophils immigrating to sites of inflammation.
ABSTRACT
Oblective To evaluate the corneal healing of non-epuality diopter response histopathologically,immunohistochemically and ultrastructurally after excimer laser photorefractive keratectomy(PRK) with SVS APEX PLUS(Summit Technology Inc. USA) excimer laser, and the effects of corticosteroid on the healing. Methods PRK on 6 white rabbits(12 eyes) was performed on right eye of the rabbit for an attampted correction of -4.00 diopter and on left eye for an attempted correction of -8.00 diopter. The rabbits were divided into 2 groups randomly and each group included 6 eyes: Group FLM (3 rabbits, 6 eyes) and group CM (3 rabbits, 6 eyes). Fluoromethalone was given to group FLM,and chloromycetin to group CM. On 10d, 30d and 100d ,the eyes of one rabbit in each group were enucleated randomly. Half of each cornea was prepared for electron microscope observation (SEM and TEM)and the rest embedded in OCT compound for immunohistochemical study to examine Ⅲ -C and FN. Results All eyes were reepithelialized within 3d after PRK. Subepithelial corneal haze was observed on 15d,which was dominant on 30 or 60d. On 100d postoperatively,corneal hazes of 11 eyes were grades 0 or 0. 5,only 1 eye(the left eye of group CM) was denser haze (grade 1). On 3d postoperatively, one or two layers of corneal epithelial cell covered the ablation zone. On 30d after PRK,the epithelial cells showed hyperplastic changes. The cells were larger and increased from normal 5 or 6 layers to 7 or 8 layers of cells on 100d after PRK,epithelium was clear with more bright epithelium. Microplicae and microvilli were less than before. The expression of Ⅲ -C and FN in group CM was significantly more evident than that in group FLM. Conclusion The study show that despite recovery of a continuous and smooth epithelial layer and nearly normal corneal tissues on 100d after PRK,abnormalities of both epithelium and superficial stroma can be detected in the area of ablation. The ablation depth of stroma can influence the appearance of corneal haze after PRK. The microplicae and microvilli of rabbit cornea epithelium become less after PRK,which can be one of cause leading to ocular dry sensation in some patients.
ABSTRACT
Objective To evaluate the efficacy and accuracy of photorefractive keratectomy(PRK) for myopia and myopic astigmatism.Methods SVS APEX PLUS excimer laser with a wave length of 193nm(Summit Technology Inc.Waltham,Mass,USA) was applied.Three hundred and sixteen myopic eyes of 168 patients were treated with PRK from Suptember 1996 to October 1997,and 260 eyes(84%) of 150 patients were followed-up for more than three month,including male 68(116 eyes,40.1%) and female 82(144 eyes,59.9%).The preoperative spherical equivalent refractive errors ranged from -1.25D to -10.00D(mean -4.67D± 1.63D),and astigmatism ranged from 0 to -2.00D(mean -0.33D±0.45D). The patients were divided into two groups according to the refraction:group A(from -1.25D to -5.90D) and group B(from -6.00D to -10.00D).The number of eyes in the two groups were 220 and 40,respectively.Results In group A,After 10 days,68.9% had the uncorrected visual acuity(UCVA) equal to or one line better or lower than the preoperative best corrected visual acuity(BCVA).After 1 ,3,6 and 12 months,90%,96%,95% and 94% had the UCVA equal to or one line better or lower than the preoperative BCVA,respectively.In group B,after 10 days,1,3,6 and 12 months,UCVA equal to or one line better or lower than the preoperative BCVA occurred in 35.9%,83.0%,87.0%,86.0% and 84.0% of the patients respectively. Most of the haze showed 0.5～1 grades except 3 eyes with the haze of 2 grade at 3 or 6 months and it changed to l and 0.5 grade respectively at one year.after 10 days and 1,3,6,12 months postoperatively,the corneal haze was noted in 32.9%,84.8%,62.8%,9.0%and 2.8% of the treated eyes respectively.Conclusion We found that the 193nm excimer laser PRK is a predictable,safe,stable,and effective refractive surgery for correcting myopia up to-10.00D in Chinese patients,and the effect is better in myopia lower than -6.00D.
ABSTRACT
To evaluate the efficacy and accuracy of photorefractive keratectomy (PRK) for myopia and myopic astigmatism .SVS APEX excimer laser with a wave length of 193 nm(Sumit Technology Inc. Waltham, Mass, USA) was applied. 316 myopic eyes of 168 patients were treated with PRK between September, 1996 and October, 1997, and 260 eyes (84%) of 150 patients were followed for more than three months, including male 68(116 eyes, 40.1%) and female 82(144 eyes, 59.9%). The preoperative spherical equivalent refractive errors ranged from -1.25D to -10.00 (mean -4.67±1.63D), and astigmatism ranged from 0 to -2.00D (means -0.33±0.45D). We divided the patients into two groups according to the referaction: group A (from -1.25D to -5.90D) and group B(from -6.00 to -10.00D). The number of eyes in the two groups were 220 and 40 respectively. In group A, on the 10th day, 68.9% has the uncorrected visual acuity (UCVA) equal to or one line better or lower than the preoperative best corrected visual acuity (BCVA). In 1,3,6 and 12 months, 90%, 96%, 95% and 94% had the UCVA equal to or one line better or lower than the preoperative BCVA respectively. In group B, on the 10 th day, in 1,3,6 and 12 months, UCVA equal to or one line better or lower than the preoperative BCVA occurred in 35.9%, 83%, 87%, 86%, and 84% of the cases respectively. Most of the haze showed 0.5～1 grades except 3 eyes with the haze of 2 grade in 3 or 6 months and it changed to 1 and 0.5 grade respectively within one year. In 10 days and 1,3,6,12 months postoperatively, the corneal haze was noted in 32.9%, 84.8%, 62.8%,9.0% and 2.8% of the treated eyes respectively. We found that 193 nm excimer laser PRK was a predictable, safe, stable, and effective refractive surgery for correcting myopia up to -10.00D in Chinese patients, and the effect was better in myopia lower than -6.00D.