ABSTRACT
Abstract Update in Royal Jelly Roongtawan Supabphol BSc (Pharm), MSc (Physiology), PhD Department of Physiology, Faculty of Medicine, Srinakharinwirot University Royal jelly is the nutritional health food and was fed as the principal food source for the queen honeybee. Several nutrients were studied, such as lipid, carbohydrate. protein, vitamins and mineral. Many kinds of protein were found, these might lead to the low incidence of allergic reaction. Most allergic reactions from royal jelly were from respiratory system, bronchospasm and asthma. Many pharmacological effects of royal jelly were discussed, anticarcinogenic, antihyperlipidemia. antiinflammation, wound healing. etc ., few adverse effects were reported. There should not have any problem if it was carefully consumed with the good suggestion.Vajira Med J 2002 ; 46 : 79 - 85
ABSTRACT
The aim of this study was to investigate the antioxidant activity of the aqueous extracts of leaves of Siamese neem tree [Azadirachta indica A. Juss var. siamensis Valeton] from several extracting and drying methods using 2,2-diphenyl-1-picrylhydrazyl [DPPH]-scavenging assay. The leaves of Siamese neem tree were extracted using percolation, decoction, maceration, soxhlet extraction, freeze drying or spray drying methods. The extract was tested for antioxidant activity using DPPH-scavenging assay. Thin-layer chromatography of the extract from decoction was also investigated. The freeze drying method gave the highest yield [51.50%, w/w] of crude extract, while decoction gave the most effective DPPH-scavenging activity [EC[50]: 31.4 micro g/ml]. Thin-layer chromatography analysis was used to screen the leaf extract obtained using decoction, and the chromatogram showed spots corresponding to quercetin and rutin flavonoids which exhibited antioxidant activities [EC[50]: 2.29 and 34.67 micro g/ml, respectively]. Siamese neem tree leaf extracts possessed free radical scavenging activity against the DPPH radical. The most active extract was obtained with the leaf decoction method. It showed antioxidant activity with EC[50] of 31.4 micro g/ml