Nivel de ansiedad según el trimestre del embarazo en un establecimiento de salud de atención primaria / Level of anxiety according to gestational trimester in a primary care health establishment

Resumen OBJETIVO: Determinar el nivel de ansiedad de las embarazadas conforme al trimestre de la gestación. MATERIALES Y MÉTODOS: Estudio observacional, correlacional y transversal efectuado en mujeres en curso del primer, segundo y tercer trimestre del embarazo atendidas en el Centro de Salud Base San Martín de Porres, Lima, Perú, entre diciembre de 2021 y febrero de 2022, a quienes se les aplicó el instrumento de tamizaje de ansiedad prenatal (Perinatal Anxiety Screening Scale [PASS]) y su variación según el trimestre del embarazo con las pruebas de χ2, Kruskall-Wallis y ANOVA. RESULTADOS: Se estudiaron 153 pacientes en los tres trimestres del embarazo en quienes se encontró: ansiedad prenatal en el 69.9% (n = 107); ansiedad leve en 45.8% (n = 70) y ansiedad moderada-severa en 24.2% (n = 37). El porcentaje es mayor de 100% porque no en todos los trimestres se encontraron síntomas depresivos. Solo 30.1% (n = 46) de las embarazadas no tuvieron síntomas de ansiedad prenatal. La puntuación de ansiedad global mostró un comportamiento diferente según el trimestre de gestación (ANOVA: p = 0,006), con predominio de mayor ansiedad en el primer trimestre seguido del tercero. La comparación por parejas solo mostró diferencias significativas en la puntuación de ansiedad entre el primer y el tercer trimestre (test de Tukey: p > 0.05). CONCLUSIONES: Más de la mitad de las embarazadas cursó con ansiedad, sobre todo en el primer y tercer trimestre y menor en el segundo.

Abstract OBJECTIVE: To determine the anxiety level of pregnant women according to the trimester of gestation. MATERIALS AND METHODS: Observational, analytical and prospective study of a random sample of pregnant women attended in a first level facility of the Ministry of Health (MINSA). Anxiety was assessed with the Perinatal Anxiety Screening Scale (PASS) and its variation according to the trimester of pregnancy with the χ2, Kruskall-Wallis and ANOVA tests. RESULTS: The prevalence of anxiety during pregnancy was 69.9% (n = 107) in a sample of 153 patients. Except for the social anxiety dimension (p > 0.05), significant differences were found in the scores of the anxiety dimensions by trimester. They were highest in the first, followed by the third trimester (p < 0.05). The global anxiety score showed different behavior by trimester of pregnancy (ANOVA: p = 0.006), with predominance of higher anxiety in the first trimester followed by the third trimester. Comparison by couples only showed significant differences in anxiety score between the first and third trimester (Tukey test: p > 0.05). CONCLUSIONS: More than half of the pregnant women had anxiety, especially in the first and third trimester and less in the second trimester.

Caracterización de los determinantes sociales de la salud del envejecimiento activo en estudios enfocados a la calidad de vida: mapeo sistemático / Characterization of the social determinants of health in active aging in studies focused on quality of life: systematic mapping

Resumen El aumento demográfico de adultos mayores se está presentando acompañado de condiciones socioeconómicas y de salud desfavorables, que afectan su calidad de vida. Esta situación puede paliarse a través del marco del envejecimiento activo, el cual aporta lineamientos alternativos que pueden resultar eficaces para contrarrestarlos. La Organización Mundial de la Salud establece que el envejecimiento activo tiene seis Determinantes Sociales de la Salud (DSS) que han sido abordados desde diferentes áreas por la comunidad científica. El objetivo del presente trabajo fue identificar y caracterizar el tipo de estudios enfocados a la calidad de vida, desde el envejecimiento activo, y clasificarlos de acuerdo con los DSS. Se revisaron las bases de datos Ebscohost, Scopus, Oxford, Redalyc y Sage Journals. Los descriptores utilizados fueron "envejecimiento activo" y "calidad de vida", en inglés y español. La búsqueda del mapeo sistemático comprendió enero 2015 a marzo 2022. Se localizaron 91 artículos en los que se evaluó la calidad de vida, desde la perspectiva del envejecimiento activo y la mayoría (81.3 %) fueron estudios transversales. Se identificaron 3 intervenciones psicológicas, con evaluación antes y después de las sesiones, abordando los DSS relacionados con los factores personales, como salud psicológica, percepción positiva con la actividad física y función cognitiva. Así mismo, los factores conductuales, como estilos de vida para la prevención de riesgo de caídas y mejora de la vida en general, que podrían condicionar la calidad de vida. El principal alcance temático de los estudios fue la vinculación existente entre la calidad de vida y los DSS relacionados con los factores personales y con el entorno social. Se requieren más estudios interdisciplinarios que ayuden a generar políticas públicas que impacten favorablemente en este sector poblacional.

Abstract The demographic increase of elderly people is taking place along with unfavorable socioeconomic and health conditions, which affect their quality of life. This situation can be alleviated through an active aging framework, which provides alternative guidelines that can be effective in counteracting them. The World Health Organization establishes that active aging has six Social Determinants of Health (SDH) that have been approached from different areas by the scientific community. The objective of this work was to identify and characterize the type of studies focused on quality of life, from active aging, and classify them according to the SDH. Ebscohost, Scopus, Oxford, Redalyc and Sage Journals databases were reviewed. The descriptors used were "active aging" and "quality of life", in English and Spanish. The systematic mapping search comprised from January 2015 to March 2022. 91 articles were located where quality of life was evaluated from the perspective of active aging and the majority (81.3 %) were cross-sectional studies. Three psychological interventions were identified, with an evaluation before and after the sessions, comprising the SDH related to personal factors, such as psychological health, positive perception with physical activity and cognitive function. Likewise, behavioral factors such as lifestyles for the prevention of risk of falls and general life improvement which could condition the quality of life. The main thematic scope of the analyzed studies was the existing relationship between quality of life and the SDH related to personal factors and the social environment. Further interdisciplinary studies are required to contribute to the creation of public policy that impacts favorably this sector of the population.

Root canal microbiota as an augmented reservoir of antimicrobial resistance genes in type 2 diabetes mellitus patients

Abstract Antimicrobial resistance is a global public health problem. Root canal microbiota associated with apical periodontitis represents a well-known reservoir of antimicrobial resistance genes (ARGs). However, the effect of type 2 diabetes mellitus (T2DM) in this reservoir is unknown. This study aimed to establish if root canal microbiota associated with apical periodontitis in T2DM patients is an augmented reservoir by identifying the prevalence of nine common ARGs and comparing it with the prevalence in nondiabetic patients. Methodology This cross-sectional study included two groups: A T2DM group conformed of 20 patients with at least ten years of living with T2DM and a control group of 30 nondiabetic participants. Premolar or molar teeth with pulp necrosis and apical periodontitis were included. A sample was collected from each root canal before endodontic treatment. DNA was extracted, and ARGs were identified by polymerase chain reaction. Results tetW and tetM genes were the most frequent (93.3 and 91.6%, respectively), while ermA was the least frequent (8.3%) in the total population. The distribution of the ARGs was similar in both groups, but a significant difference (p<0.005) was present in ermB, ermC, cfxA, and tetQ genes, being more frequent in the T2DM group. A total of eighty percent of the T2DM patients presented a minimum of four ARGs, while 76.6% of the control group presented a maximum of three. Conclusions Root canal microbiota associated with apical periodontitis in T2DM patients carries more ARGs. Therefore, this pathological niche could be considered an augmented reservoir.

Impacto psicológico de la pandemia COVID-19 en cinco países de Latinoamérica / Psychological impact of the COVID-19 pandemic on five Latin American countries

Resumen Introducción: El distanciamiento social y la cuarentena han probado tener efectos negativos en la salud mental de las poblaciones, a saber: miedo, ansiedad, depresión y sintomatología de estrés postraumático. La resiliencia emerge como variable amortiguadora del impacto. El objetivo del estudio fue comparar el impacto psicológico del COVID-19 en varios países latinoamericanos. Método: se obtuvo una muestra de 1184 participantes de México, Cuba, Chile, Colombia y Guatemala; cuya edad osciló entre 18 y 83 años (M = 38.78, DT = 13.81). Se aplicó una encuesta sobre síntomas médicos asociados al COVID-19 con tres instrumentos para evaluar: (1) síntomas de depresión, ansiedad y estrés, (2) impacto del evento y (3) resiliencia. Resultados: Las personas más jóvenes, con mayor cantidad de síntomas médicos y con mayores puntajes de impacto del evento tienden a presentar mayor sintomatología depresiva, ansiosa y estrés, siendo el impacto del evento el predictor más determinante. La resiliencia fue el predictor protector contra la depresión, ansiedad y estrés. Conclusiones: Los resultados muestran las diferencias en la respuesta psicológica ante la pandemia del COVID-19 en cada país, y sugieren la necesidad del desarrollo de políticas públicas enfocadas en la prevención y la promoción de la salud integral ante emergencias sanitarias.

Abstract Introduction: Social distancing and quarantine have proven to have negative effects on the mental health of populations, namely fear, anxiety, depression and post-traumatic stress symptoms. Resilience emerges as a buffering variable for such impact. The objective of this study was to compare the psychological impact of COVID-19 in several Latin American countries. Method: A sample of 1184 participants from Mexico, Cuba, Chile, Colombia and Guatemala was obtained; whose age ranged from 18 to 83 years old (M = 38.78, SD = 13.81). A survey on medical symptoms associated with COVID-19 and three instruments to evaluate: (1) depression, anxiety and stress, (2) impact of the event and (3) resilience were administered. Results: Younger people, with more symptoms associated with COVID-19 and those who reported higher scores of impact of event tended to present greater depressive, anxious and stress symptomatology. The impact of the event was the most determinant predictor. Resilience was protective against the impact of event, depression, anxiety and stress. Conclusions: The results show the differences in the psychological response to COVID-19 in each country and suggesting the need to develop public policies focused on prevention and promotion of integral health when facing sanitary emergencies.

Translation and cross-cultural adaptation into Brazilian Portuguese of the Mood and Feelings Questionnaire (MFQ) - Long Version / Tradução e adaptação transcultural para o português brasileiro do instrumento Mood and Feelings Questionnaire (MFQ) - Versão Longa

Abstract Introduction Major depressive disorder (MDD) is prevalent among young people, with a high incidence during adolescence. It is, therefore, important to have reliable instruments to capture the construct of depression in this population. The objective of the present work is to describe the process of translation and cultural adaptation of the Mood and Feelings Questionnaire (MFQ) - Long Version, into Brazilian Portuguese. Method We followed the International Society for Pharmacoeconomics and Outcomes Research (ISPOR) guidelines for translation and cultural adaptation, including the steps of preparation, forward translation, reconciliation, back-translation, back-translation review, harmonization, cognitive debriefing, review of cognitive debriefing results and finalization, proofreading and final report. Cognitive debriefing was conducted in a sample of adolescent patients and their respective caregivers at mental health clinics affiliated with the Brazilian public health system. Results Results suggest that the items were well understood and that the MFQ seems to be an appropriate instrument for use with Brazilian adolescents and caregivers. Conclusions The Brazilian Portuguese MFQ - Long Version constitutes an adequate tool for the assessment of depression among adolescents. Future studies are required to evaluate psychometric properties of the instrument.

Resumo Introdução O transtorno depressivo maior (TDM) é prevalente em jovens, com alta incidência durante a adolescência. Portanto, é importante que instrumentos confiáveis estejam disponíveis para avaliar o construto da depressão nessa população. O objetivo do presente trabalho é descrever o processo de tradução e adaptação cultural do Mood and Feelings Questionnaire (MFQ) - Versão Longa para o português brasileiro. Método Foram utilizadas as diretrizes da International Society for Pharmacoeconomics and Outcomes Research (ISPOR) para tradução e adaptação cultural, incluindo as etapas de preparação, tradução, reconciliação, retrotradução, revisão da retrotradução, harmonização, estudo piloto, revisão dos resultados do estudo piloto, revisão final e relato final. A etapa de estudo piloto foi conduzida em uma amostra de pacientes adolescentes e seus respectivos cuidadores em clínicas de saúde mental afiliadas ao Sistema Único de Saúde. Resultados Os resultados sugeriram que os itens foram bem compreendidos, e que o MFQ parece ser um instrumento apropriado para uso com adolescentes e cuidadores brasileiros. Conclusões A versão traduzida para o português brasileiro do MFQ - Versão Longa constitui um instrumento adequado para a avaliação da depressão em adolescentes. Futuros estudos são necessários para avaliar as propriedades psicométricas da escala.

Ursane derivatives isolated from leaves of Hylocereus undatus inhibit glycation at multiple stages / 中国天然药物

The present study was designed to evaluate the therapeutic potential of bioactive compounds from chloroform extract of the leaves of Hylocereus undatus in the formation of advanced glycation end products (AGEs) in vitro. Bioactivity-guided fractionation of chloroform extract from Hylocereus undatus afforded two novel 12-ursen-type triterpenes, 3β, 16α, 23-trihydroxy-urs-12- en-28-oic acid (1) and 3β, 6β, 19α, 22α-tetrahydroxy-urs-12-en-28-oic acid (2), as well as four known triterpenes 2α, 3β, 23-tetrahydroxy-urs-11-en-28-oic acid (3), 3β-acetoxy-28-hydroxyolean-12-ene (4), 3β, 16α-dihidroxyolean-12-ene (5) and 3β-acetoxy-olean-12-ene (6). Our results revealed that triterpenes 1-3 were able to inhibit the formation of AGEs in all tested assays. The data indicated that the triterpenes had inhibitory activity at the múltiple stages of glycation and that there might be a high potential for decreasing protein oxidation and protein glycation that can enhance glycative stress in diabetic complications.

Ursane derivatives isolated from leaves of Hylocereus undatus inhibit glycation at multiple stages / 中国天然药物

The present study was designed to evaluate the therapeutic potential of bioactive compounds from chloroform extract of the leaves of Hylocereus undatus in the formation of advanced glycation end products (AGEs) in vitro. Bioactivity-guided fractionation of chloroform extract from Hylocereus undatus afforded two novel 12-ursen-type triterpenes, 3β, 16α, 23-trihydroxy-urs-12- en-28-oic acid (1) and 3β, 6β, 19α, 22α-tetrahydroxy-urs-12-en-28-oic acid (2), as well as four known triterpenes 2α, 3β, 23-tetrahydroxy-urs-11-en-28-oic acid (3), 3β-acetoxy-28-hydroxyolean-12-ene (4), 3β, 16α-dihidroxyolean-12-ene (5) and 3β-acetoxy-olean-12-ene (6). Our results revealed that triterpenes 1-3 were able to inhibit the formation of AGEs in all tested assays. The data indicated that the triterpenes had inhibitory activity at the múltiple stages of glycation and that there might be a high potential for decreasing protein oxidation and protein glycation that can enhance glycative stress in diabetic complications.

Comparison of subjective health between personality prototypes extracted from general population of Mexico / Comparação da saúde subjetiva entre protótipos de personalidade em população geral do México / Comparación de la salud subjetiva entre prototipos de personalidad recuperados en población general de México

Abstract The objective of this study was to extract personality prototypes from general population of Mexico and to compare subjective health indicators between these prototypes. Participants were 994 individuals (aged 14 to 63 years). Five personality traits (Neuroticism, Extraversion, Openness, Conscientiousness, and Agreeableness) were assessed with the NEO-FFI. Subjective indicators of health (self-rated health and psychological distress) were assessed with a question regarding health status and the GHQ-12. To verify the consistency of the prototypes, the sample was divided into two age groups, young (14 to 25 years) and mature (26 to 63 years.). Three stable prototypes were recovered from both groups. Resilient individuals (low neuroticism and high in other traits) had the best subjective health; the Non-Resilient individuals (high neuroticism and low in other traits) had the worst subjective health; and Self-Disciplined individuals (high conscientiousness and medium scores in other traits) were in the middle of these extremes in subjective health. Self-discipline and resilience were most discriminative in terms of subjective health. Findings are discussed in terms of the generalization of prototypes across cultures.

Resumo O objetivo deste estudo foi obter protótipos da personalidade em população geral do México e comparar a saúde subjetiva entre eles. No total, participaram 994 indivíduos de 14 a 63 anos. Avaliaram-se cinco traços de personalidade (extroversão, neuroticismo, abertura à experiência, conscienciosidade e amabilidade) com o NEO-FFI, e a saúde subjetiva com o GHQ-12, e uma pergunta sobre o estado de saúde. Para corroborar a consistência dos protótipos, dividiu-se a amostra em dois grupos de idade: jovem (de 14 a 25 anos) e adulto (de 26 a 63 anos). Como resultado, obtiveram-se três protótipos em ambos os grupos: as pessoas resilientes -baixo neuroticismo e alto nos demais traços-, os que tiveram a melhor saúde subjetiva; as não resilientes -alto neuroticismo e baixo no restante dos traços-, que apresentaram a pior saúde subjetiva, e as pessoas disciplinadas -alto em conscienciosidade e média nos outros traços-, que apresentaram uma saúde subjetiva intermediária em comparação com os outros protótipos. Finalmente, a autodisciplina e a resiliência foram os traços que melhor discriminaram a boa saúde. Os achados são discutidos em termos da generalização de protótipos através de culturas.

Resumen El objetivo de este estudio fue obtener prototipos de personalidad en población general de México y comparar la salud subjetiva entre ellos. En total, participaron 994 individuos de 14 a 63 años de edad. Se evaluaron cinco rasgos de personalidad (Neuroticismo, Extraversión, Apertura, Responsabilidad y Amabilidad) con el NEO-FFI, y la salud subjetiva con el GHQ-12 y una pregunta sobre el estado de salud. Para corroborar la consistencia de los prototipos se dividió la muestra en dos grupos de edad: joven (de 14 a 25 años) y maduro (de 26 a 63 años). Y como resultado se recuperaron tres prototipos en ambos grupos: las personas Resilientes -bajo neuroticismo y alto en el resto de los rasgos-, quienes tuvieron la mejor salud subjetiva; las No-Resilientes -alto neuroticismo y bajo en el resto de los rasgos-, que presentaron la peor salud subjetiva; y las personas Disciplinadas -alto en responsabilidad y promedio en los otros rasgos-, que presentaron una salud subjetiva intermedia en comparación con los otros prototipos. Finalmente, la autodisciplina y la resiliencia fueron los rasgos que discriminaron mejor la buena salud. Los hallazgos se discuten en términos de la generalización de prototipos a través de culturas.

Effects of bixin in high-fat diet-fed-induced fatty liver in C57BL/6J mice

Objective To evaluate the anti-obesity activity of bixin (BIX) on C57BL/6J mice which were fed a high-fat diet (HFD) and to determine the mechanism of this effect. Methods C57BL/6J mice were separately fed a high-calorie diet or a normal diet for 8 weeks, then they were treated with BIX for another 13 weeks. After administration for 13 weeks, the animals were sacrificed. Body adiposity, serum lipid level, and insulin resistance were evaluated. In addition, a histological assay of pancreas and liver, an evaluation of the inhibitory properties on pancreatic lipase, and α-amylase were conducted. Results Administration of BIX significantly decreased the body weight gain, adipocyte size, fat pad weights, hepatic lipid levels in HFD-induced obese mice. In addition, reduced liver weight exhibited decreased serum leptin levels, malic enzyme, glucose-6-phosphate dehydrogenase, hepatic fatty acid synthase, aspartate aminotransferase, alanine aminotransferase and hepatic phosphatidate phosphohydrolase activity. However, superoxide dismutase, catalase, glutathione peroxidase, and glutathione levels were increased in hepatic tissue. BIX also decreased lipid and carbohydrates absorption due to inhibition of pancreatic lipase and α-amylase. Long term supplementation of BIX significantly decreased hyperlipidemia, insulin resistance and glucose level. Decreased levels of hepatic steatosis and the islets of Langerhans appeared less shrunken in HFD-fed mice. Conclusions The antiobesity effect of BIX appears to be associated at least in part, to its inhibitory effect on lipids and carbohydrate digestion enzymes such as pancreatic lipase, α-glucosidase, and α-amylase. The results suggested that BIX also act as an antioxidant and may treat visceral obesity normalizing glucose levels, improving insulin resistance and increasing energy expenditure. Therefore, achiote which has a main component, the carotenoid BIX, could be a viable food for the treatment of obesity and diabetes.

Antimicrobial activities of diterpene labdane from seeds of Byrsonima crassifolia / Actividad antimicrobial de diterpenos labdanos de la semilla de Byrsonima crassifolia

Two compounds from the hexane extract of seeds of Byrsonima crassifolia were isolated and their structures elucidated using extensive spectroscopic analyses. These compounds are derived from the new labdane diterpene Labda-17-(1) and the known antimicrobial Labda-8 (17)-(2). The present study was aimed to study the effect antimicrobial of novel diterpene 1 against bacterial pathogens showed a moderate activity with MIC values 18.79-70.12 ug/ml and a MBC ranging between 250-1000 ug/ml against all assayed microorganisms.

Se aislaron dos compuestos del extracto de hexano de semillas de Byrsonima crassifolia y sus estructuras se dilucidaron por medio de extensos análisis espectroscópicos. Estos compuestos derivados del labdano corresponden al nuevo diterpeno Labda-17- (1) y el conocido antimicrobiano Labda-8(17)-(2). En el presente estudio se estudió el efecto antimicrobiano del nuevo diterpeno 1 sobre algunas bacterias patógenas mostrando sobre de estas una actividad moderada, con valores de MIC de 18.79-70.12 ug/ml y un rango de MBC que oscila entre 250-1000 ug/ml frente a todos los microorganismos ensayados.

Caracterización de la sobrecarga y de los estilos de afrontamiento en el cuidador informal de pacientes dependientes / Characterization of overburdening and coping styles in the informal care of dependent patients

Introducción: los recursos y estrategias son elementos de los que el cuidador dispone para tratar de amortiguar el posible impacto de la situación de cuidado. Métodos: Se realizó un estudio descriptivo transversal con el propósito de caracterizar la sobrecarga y los estilos de afrontamiento de los cuidadores informales en el área de salud "Héroes de Girón", del municipio Cerro. Se aplicó una encuesta que incluyó la entrevista de carga del cuidador y el cuestionario COPE. Resultados: la sobrecarga se presentó en el 47,6 % de los cuidadores. Las mujeres representaron el 90 % de esta, y recayó en el grupo de 60 años y más (50 %), casados (50 %), técnicos medios (30 %), los que cuidaban mayor cantidad de horas (63,6 %) y que no habían participado en algún programa educativo (95 %). Los estilos de afrontamiento más frecuentemente usados en los cuidadores fue la aceptación (3,70), el afrontamiento activo (3,53) y la reformulación positiva (3,01). El tipo de afrontamiento más frecuentemente usado fue el conductual activo (3,23). Conclusiones: La sobrecarga se presenta en poco menos de la mitad de los cuidadores, con predominio en las mujeres, en el grupo de 60 años y más, en el nivel técnico medio y en los casados, que cuidan más de 12 horas y que no han participado en ningún programa educativo. Las estrategias de afrontamiento más usadas son las activas dirigidas a resolver los problemas que se presentan con el cuidado, independientemente de la edad, el sexo y el nivel educacional.

Introduction: resources and strategies are elements the caregiver has to try to weaken the possible impact of caregiving situation. Methods: a transversal descriptive study was conducted with the objective to characterize overburdening and coping styles of informal caregivers in "Héroes de Girón" health area, Cerro municipality. A survey was applied which included Caregiver Burden Interview and COPE questionnaire. Results: overburdening was present in the 47, 6 % of caregivers. Women represented the 90 % of this, and it fell in the age group of 60 years or older (50 %), married (50 %), technicians (30 %) those who cared most hours (63.6 %) and those who had not participated in any educational program (95 %). The most frequent coping styles used by caregivers were: acceptance (3.70), active coping (3.53) and positive reframing (3.01). The most frequently used coping style was the active behavioral one (3.23). Conclusions: overburdening is present in slightly less than half of caregivers, it predominates in women, in the age group of 60 years and older, in intermediate levels, in married people, people who care more than 12 hours and in those who have not participated in any educative program. The most used coping styles are the active ones directed to solve the problems which arise with care, regardless the age, sex and educational level.

Inhibition of advanced glycation end products formation by stilbene and phenanthrene derivatives from Prosthechea michuacana in vitro and in vivo / Inhibición de la formación de subproductos en la glicosilación avanzada por derivados de estilbeno y fenantreno de Prosthechea michuacana, in vitro e in vivo

In our previous study, we isolated from chloroform extract of the bulbs of orchid P. michuacana, three antioxidant compounds: two stilbene alpha-alpha´-dihydro, 3´,5´,2-trimethoxy-3-hydroxy-4-acetyl-4´-isopentenyl stilbene, 5-[2-(3-hydroxy-5-methoxyphenyl)ethyl]-2-methoxyphenol (gigantol) and one phenanthrene 4,6,7-trihydroxy-2-methoxy-8-(methylbut-2-enylphenanthren-1-1´-4´,6´,7´-trihydroxy-2´-methoxy-8´-(methylbut-2´-enyl)-phenanthrene. Following the study, we investigated the ability of isolated compounds to inhibit advanced glycation in vitro. Bovine serum albumin was glycated in the presence of glucose or methylglyoxal. Amadori-rich protein was prepared by dialyzing lysozyme that had been glycated by ribose. We also evaluated renal function by checking formation of advanced glycation and tail tendon collagen quality in streptozotocin-induced diabetic mice. Also determined the effect on LDL and hemoglobin. Compounds can efficiently inhibit the formation of AGEs by trapping reactive methylglyoxal and showed potent anti-Amadorin activity. Also exhibited a significant inhibitory activity on the glycated hemoglobin (GHb and HbA1c). Compounds showed a protective renal effect and reduction in mice tail tendon collagen. Also the tested compounds are potent agents for protecting LDL against oxidation and glycation. We concluded that compounds from P. michuacana are potent antiglycation agents, which can be of great value in the prevention of diabetic glycation-associated-pathogenesis.

En un estudio anterior, aislamos del extracto clorofórmico de los bulbos de la orquídea Prosthechea michuacana, tres compuestos antioxidantes: los estilbenos alfa-alfa´-dihidro, 3´,5´,2-trimethoxi-3-hidroxi-4-acetil-4´-isopentenil-stilbeno, 5-[2-(3-hydroxy-5-methoxyphenyl)ethyl]-2-methoxyphenol (gigantol) y el fenantreno 4,6,7-trihidroxi -2-methoxi-8-(metilbut-2-enilfenantren-1-1´-4´,6´,7´-trihidroxi-2´-metoxi-8´-(metilbut-2´-enil)-fenantreno. Continuando con el estudio, investigamos la capacidad de estos compuestos para inhibir la glicación avanzada in vitro. La seroalbúmina bovina se glicosiló en presencia de glucosa o metilglioxal. La reacción de Amadori se determinó con lisozima glicosilada previamente tratada con ribosa. También se evaluó la función renal mediante la formación de la glicación avanzada y la inhibición de AGEs en el ensayo sobre el colágeno del tendón de la cola en ratones con diabetes inducida con estreptozotocina. También determinamos el efecto de los compuestos aislados sobre LDL y hemoglobin. Los compuestos pueden inhibir eficazmente la formación de AGE atrapando el metilglioxal reactivo y muestran potente actividad anti Amadorin. También mostraron una actividad inhibitoria significativa en la formación de la hemoglobina glucosilada, GHB y HbA1c. Mostraron un efecto protector renal y una reducción en el colágeno glicosiladó del tendón de la cola. También estos compuestos son potentes agentes para la protección de LDL frente la oxidación y la glicación. En base a los resultados obtenidos se concluye que los compuestos aislados son potentes agentes antiglicación, que pueden ser de gran valor en la prevención de la patogénesis de la diabetes asociada a la glicación.

Estilos de afrontamiento al estrés y bienestar subjetivo en adolescentes y jóvenes / Stress coping styles and subjective well-being in adolescents and young adults / Estilo do enfrentamento o estresse e bem-estar subjetivo em os adolescentes é jóvemes

Con la investigación buscamos determinar la relación entre el nivel de Bienestar Subjetivo y los Estilos de Afrontamiento al Estrés en adolescentes y jóvenes de Colima, México. En el estudio participaron 436 estudiantes con rango de edad de 15 a 24 años. Para recabar la información utilizamos la escala de Modos de Afrontamiento de Lazarus y Folkman (versión adaptada de Sandin & Chorot, 2003) y la Escala Multidimensional para la medición del Bienestar Subjetivo (Anguas-Plata & Reyes, 1998). Los resultados indican que los adolescentes y las adolescentes mantienen un promedio más alto de bienestar subjetivo que los sujetos jóvenes, y que el estilo de afrontamiento que más utilizan es el de aceptación de la responsabilidad, mientras que el menos utilizado es la huídaevitación.

The aim of this study was to determine the relationship between the level of subjective well-being and stress coping styles in adolescents and young adults from Colima, México. A descriptive correlational study was conducted involving 436 participants, all of whom were high- school or undergraduate students. Data were collected using the Ways of Coping Questionnaire by Folkman and Lazarus (adapted version by Sandin & Chorot, 2003) and the Multidimensional Scale for Measuring Subjective Well-Being (Anguas-Platas & Reyes, 1998). Our findings indicated that teenagers showed a higher subjective well-being than their undergraduate counterparts. In both, adolescents and young adults, the most commonly used coping style was "Acceptance of Responsibility", whereas the least used coping style was "Escape- Avoidance".

A investigação busca determinar a relação entre o nivel de Bem-estar Sujetivo é os Estilos de Enfrentamento do Estresse em os adolescentes é jóvenes de Colima, México. No estudio participaron 436 estudiantes com posição das idades entre 15 a 2ª anos. Para exigir a informação, utilizou-se a escala de Modos de Enfrentamento de Lazarus é Folkman) versão adaptada de Sandin é Chorot, 2003) é a Escala Multidimensão para a medição do Bem-estar Subjetivo (Anguas-Plata é Reyes, 1998). Os resultados indican que os adolecscentes mantenhem a média máis alto de bem-estar subjetivo que os jóvenes é que o estilo de afrentamento que mais usan e aceptação da responsabilidade, enquanto que os menos utilizado e a fuga-evitação.

Caracterización del cuidado informal de pacientes dependientes en el policlínico Héroes de Girón en el 2009 / Characterization of informal care of dependent patients at the polyclinic Héroes de Girón in 2009

Introducción: con el progresivo envejecimiento de la población y la mayor supervivencia de pacientes con enfermedades crónicas y discapacidades, no solo aumenta el número de personas que necesitan cuidados, sino que además existe una mayor exigencia en la prestación de servicios. Objetivo: caracterizar el cuidado informal de pacientes dependientes en el policlínico Héroes de Girón, en el 2009. Métodos: se realizó un estudio descriptivo, transversal. Se aplicó una encuesta que incluye la entrevista de carga del cuidador. La muestra estuvo constituida por 42 cuidadores informales de pacientes dependientes del policlínico Héroes de Girón, del municipio Cerro, que corresponde con el total de cuidadores informales del área de salud en el momento del estudio. Resultados: el sexo femenino predominó en los pacientes dependientes 30 (71,43 porciento) y en los cuidadores 35 (83,33 porciento), 18 (42,86 porciento) de los cuidadores eran esposas, su edad media era de 52 años, fueron amas de casa 17 (40,48 porciento), y 29 (69,05 porciento) le dedicaba al cuidado más de 12 horas al día. La sobrecarga se presentó en 22 cuidadores (52,38 porciento). Conclusiones: menos de la mitad de los cuidadores presentó sobrecarga; predominaron las mujeres de 60 años de edad o más (amas de casa, hijas o esposas) que dedicaban 12 horas diarias o más ese cuidado. Más del 90 porciento no ha participado en ningún programa educativo

Introduction: with an aging population and increased survival of patients with chronic diseases and disabilities, the number of people needing care not only increases, but also the provision of services increases in demand. Objective: to characterize the informal care of dependent patients at the polyclinic Héroes de Girón, in 2009. Methods: we conducted a cross-sectional study. We used a survey including the caregiver burden interview. The sample consisted of 42 caregivers of dependent patients at the polyclinic Héroes de Girón, Cerro municipality; they correspond to the total of informal caregivers in the health area at the time of this study. Results: most of the dependent patients 30 (71.43 percent) and caregivers 35 (83.33 percent) were females, out of them 18 (42.86 percent) were wives, the average age is 52 years, 17 (40.48 percent) were housewives and 29 (69.05 percent) devoted more than 12 hours a day to this care. The overload was present in 22 caregivers (52.38 percent). Conclusions: less than half of caregivers presented overload; most of them were women aged 60 and over. They were housewives, daughters, or wives. They devoted more than 12 hours a day to this care. Over 90 percent have not participated in any educational program

Anti-diabetic activity of an hexane extract of Prosthechea michuacana in streptozotocin-induced diabetic rats / Actividad antidiabética de un extracto de hexane de Prosthechea michuacana en ratas diabéticas inducidas por estreptozotocina

In this study we investigated the antihyperglycaemic, antihyperlipidaemic and antiglycation effects of some extracts of Prosthechea michuacana bulbs in normoglycemic and diabetic rats induced by streptozocin (STZ). Hexane, chloroform and methanol extracts of P. michuacana were screened for hypoglycemic activity, and biochemical parameters as serum triglycerides, total cholesterol, lipid peroxidation, liver glycogen, skeletal muscle glycogen levels, superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase activity in diabetic rats. Additionally we determined Glucose 6 Phosphatase and glucokinase activities in liver, inhibition of insulin and protein glycation. Glucose levels in blood plasma were determined by using GOD-POD method. Administration of chloroform and methanol extracts showed no effect on STZ induced diabetic rats (SD). On the other hand, treatment with hexane extract at 200 and 400 mg/kg doses, resulted in a reversal of diabetes and its complications. Both doses significantly brought down blood glucose concentration (35.75 and 47.78 percent in diabetic rats, 50.64 and 57.10 percent in nondiabetic rats), increased glycogenesis and decreased glyconeogenesis bringing the glucose metabolism toward normalcy. These doses also reversed the hyperlipidemia by reducing cholesterol (41.56 percent, 46.08 percent) and triglycerides (37.5 percent, 46.27 percent) and improved hepatic antioxidant enzyme activities. Its effect was compared with that of glibenclamide and tolbutamide, as reference antidiabetic drugs. The hexane extract decreased the hyperinsulinemia by 24 percent in SD and showed a significant change on AGEs formation in vitro with IC50 values of 48.3 ug/ml comparable to inhibitory effect of aminoguanidine with IC50 values of 27.2 ug/ml. It reduced HbA1C levels by 6.4 percent in chronic STZ-diabetic rats. It is concluded that hexane extract of Prosthechea michuacana bulbs possesses anti-hyperglycemic and antihyperlipemic...

En este estudio se determinaron los efectos antidiabéticos, antihiperlipidemico y glicación (AGEs) de algunos extractos de Prosthechea michuacana (PM) en ratas normoglucémicas y con diabetes inducida por estreptozotocina (STZ). Se probó el efecto de los extractos de hexano, cloroformo, metanol de PM sobre la actividad hipoglucemiante, la carga de glucosa, los parámetros bioquímicos tales como triglicéridos, niveles de colesterol total, peroxidación lipídica, glucógeno del hígado, los niveles de glucógeno muscular, niveles de superoxide dismutase, catalasa, glutation reductasa and glutation peroxidasa en ratas normales y diabéticas. También se determinó la glucosa 6 Phosphatasa y las actividades de GK en el hígado, la inhibición de la insulina y la glicosilación de las proteínas. Los niveles de glucosa sanguínea se determinaron por el método de GOD-POD. La administración de los extractos de cloroformo y metanol no presentaron ningún efecto sobre la SD, en cambio el tratamiento con el extracto de hexano (PM) a dosis de 200 y 400 mg/kg, inhibió la diabetes y sus complicaciones. Ambas dosis redujeron significativamente los niveles de glucosa sanguínea (35.75 y 47.78 por ciento en las ratas diabéticas, 50.64 y 57.10 por ciento en las ratas diabéticas), el aumento de la glucogénesis y la disminución de la gluconeogénesis conduce el metabolismo de la glucosa hacia la normalidad. Estas dosis disminuyeron la hiperlipidemia reduciendo el colesterol (41.56 por ciento, 46.08 por ciento) y los triglicéridos (37.5 por ciento, 46.27 por ciento) así como también mejoran las actividades antioxidantes de las enzimas hepáticas. Su efecto se comparó con la glibenclamida y tolbutamida, fármacos usados como antidiabeticos. El extracto de hexano disminuyo la hiperinsulinemia en un 24 por ciento en SD. PM mostró un cambio significativo in vitro sobre la formación de los AGEs con valores de IC50 de 48.3 mg/ml comparable al efecto inhibidor de la aminoguanidina con valores de IC50 de...

Attenuation of hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats by chloroform extract of fruits ofFerocactus latispinus and Ferocactus histrix / Atenuación de la hiperglucemia y la hiperlipidemia en ratas diabéticas inducida por estreptozotocina por el extracto de cloroformo de los frutos de Ferocactus latispinus y Ferocactus histrix

The hypoglycemic effects of hexane, chloroform and methanol extracts from fruits of Ferocactus latispinus and Ferocactus histrix were evaluated by oral administration to normoglucemic and streptozotocin-induced severe diabetic rats (SD). The anti-diabetic effect was examined by blood glucose, triglycerides, lipid peroxidation, total cholesterol levels in the serum, glycogen content of liver and skeletal muscles, superoxide dismutase (SOD) catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GSHPx) levels. The most active extracts were obtained with chloroform. Chloroform extracts from F. latispinus and F. histrix increased activities of SOD, GR, GSHPx and CAT, hepatic glycogen content, glucose-6-phosphatase (G6Pase) and the plasma insulin levels. They also, decreased glucokinase (GK) and TBAR (thiobarbituric acid assay). Of the two plants studied F. latispinus showed better antihyperglycemic and antihyperlipidemic effects that F. histrix. In conclusion F. latispinus and F. histrix possesses significant antihyperglycemic properties after 4 h after a single oral dose. It can also improve hyperlipidemia and hypoinsulinemia in streptozotocin-induced diabetic. These results demonstrated that F. latispinus and F. histrix typically used as a health food, has strong antidiabetic effects in vivo, thus, it may have beneficial properties in the prevention of diabetes.

Los efectos hipoglucemiantes de extractos obtenidos con hexano, cloroformo y metanol a partir de frutos de Ferocactus latispinus y Ferocactus histrix fueron evaluados por la administración oral a ratas normales y con diabetes severa (SD) inducida por estreptozotocina. Los extractos más activos fueron obtenidos con cloroformo el cuál incrementa los niveles de SOD, GR, GSHPx y el CAT, el contenido de glucógeno hepático, la glucosa-6-fosfatasa (G6Pase) y los niveles de insulina plasmática. También producen disminución de la glucoquinasa (GK) y TBARS. De las dos plantas estudiadas la F. latispinus presento mayor actividad antihiperglicemiante y antihiperlipidémicos que la F. histrix. En conclusión F. latispinus y F. histrix pueden mejorar la hiperlipidemia y la hipoinsulinemia en animales diabéticos inducida por estreptozotocina. Estos resultados demostraron que F. latispinus y F. histrix utilizadas normalmente como un alimento saludable, tiene fuertes efectos antidiabéticos in vivo, por lo tanto, pueden tener propiedades beneficiosas en la prevención de la diabetes.

Consumo de bebidas alcohólicas y ambiente social. Un enfoque multinivel / Alcohol consumption and social context. A multilevel approach

Objetivos: Tradicionalmente las variaciones en el consumo de bebidas alcohólicas se han estudiado en términos de las características individuales. Con este estudio nos propusimos valorar la contribución de los factores contextuales en conjunto con los individuales, en la cantidad de bebidas alcohólicas que los individuos consumen. Métodos: Se midieron variables en 2 niveles: individual y contextuales (vecindarios). Las primeras se obtuvieron de una muestra representativa de la población mayor de 15 años del municipio 10 de Octubre y las contextuales mediante entrevistas con los representantes del gobierno de las áreas del municipio. Se usaron 3 modelos multiniveles: modelo nulo, modelo de interceptos aleatorios y modelo de coeficientes dependientes. Resultados: Existen diferencias regionales en el consumo de bebidas alcohólicas y estas diferencias persisten después de ajustar por las características individuales, por lo que se le pueden atribuir a los contextos. De los factores contextuales estudiados, 4 tienen influencia directa en la cantidad de bebidas alcohólicas que los individuos consumen y 2 de ellos interactúan con la situación laboral de las personas. Por ejemplo, las personas que están desocupadas consumen más que las que tienen alguna ocupación, pero los desocupados que viven en vecindarios con numerosos puntos de venta de bebidas alcohólicas, consumen más que los desocupados que viven en vecindarios con menos puntos de venta. Conclusiones: Los modelos multiniveles son una herramienta importante que permiten estudiar los efectos individuales y contextuales que se relacionan con el consumo de bebidas alcohólicas. Los resultados de la aplicación de una estrategia de modelación multinivel sugieren que los factores individuales y contextuales desempeñan un papel importante en la estructuración del consumo de bebidas alcohólicas.

Objectives: Traditionally the variations in alcohol consumption have been studied in terms of individual characteristics. In the present study we intended to assess the contribution of contextual factors and individual factors as well, in the units of alcohol consumed per person per. Methods: We measured variables in two levels: individual and contextual (neighborhoods). The first ones were obtained by means of a cross-sectional survey of people 15 years an older in Havana City. Contextual variables were obtained through interviews with the representatives of the local government in the city. Three multilevel models were used: null model, random intercept models and dependent coefficients model. Results: Our findings suggest that there are variations in the average number of units consumed among neighborhoods and these differences persist after adjusting for individual characteristics; so part of the variations can be attributed to the contexts. Six contextual factors were studied. Four have direct influence in the average number of units consumed and two of them interact with the economic situation of the respondents. For example, people that are unoccupied consume more than those that have an occupation; but the unoccupied ones that live in neighborhoods with numerous alcohol outlets consume more than the unoccupied ones that live in neighborhoods with less alcohol outlets. Conclusions: The results suggest that individual and contextual factors play an important role in structuring the patterns of alcohol consumption in order to design better health promotions and prevention strategies. The high-level alcohol drinkers tend to group in neighborhoods with a large number of alcohol outlets.

Calidad de vida relacionada con la salud de los adultos mayores en el país, 2001 / Quality of life related to health of elderlies in our country, 2001

El envejecimiento poblacional del mundo y de Cuba es un fenómeno demográfico asociado con la revolución científico técnica del siglo XX; sus consecuencias unidas a características socio-demográficas pueden afectar la calidad de vida. Este trabajo pretende caracterizar la calidad de vida relacionada con la salud de los adultos mayores en el país, con los datos de la II Encuesta Nacional de Factores de Riesgo de Enfermedades no Trasmisibles de Cuba en el 2001. Se calcularon números absolutos, medias, porcentajes y Odds Ratio con sus intervalos de confianza al 95 por ciento. El efecto confusor de las variables se controló con un modelo de regresión logísitica. La calidad de vida relacionada con la salud óptima mejoró significativamente al aumentar la escolaridad de los individuos desde primaria hasta la universidad, de 36,2 a 52,6 por ciento, OR=1,1 (1,019;1,266). La empeoraron significativamente el aumentar la edad, OR=0,96 (0,953; 0,973); percibir desfavorablemente la situación económica, OR=0,61 (0,491; 0,766) y pertenecer al sexo femenino, OR=0,47 (0,416; 0,540). Algunas variables socio-demográficas influyen sobre la calidad de vida relacionada con la salud de los adultos mayores y la magnitud de esta influencia debe valorarse para el manejo de este grupo de edad.

World population aging and of Cuba is a demographic phenomenon associated with the scientific and technical revolution of XX century; its consequences joined to sociodemographic characteristics may to affect the quality of life. The aim of present paper is to characterize the quality of life related to health of elderlies in our country, to data from the II National Survey of Risk Factors of Non-communicable diseases of Cuba for 2001. Authors estimated absolute numbers, means, percentages and odds ratio with their 95 percent confidence intervals. Confuse effect of variables was controlled using a logistic regression model. Quality of life related to an optimal health significantly improved with the increasing schooling of subjects from the primary school up to university level from 36.2 percent to 52.6 percent, OR = 1.1 (1.019; 1.266). There was worsening with age increase, OR = 0.96 (9.953; 0.973); a unfavorable perception of economic situation, OR = 0.61 (0.491; 0.766) and to be of female sex, OR = 0.47 (0.416; 0.540). Some socioeconomic variables influenced on the quality of life related to health of elderlies and to magnitude of this influence must to be assessed to approach this age group.

Calidad de vida relacionada con la salud de los expuestos activamente al tabaco. Cuba, 2001 / Quality of life related to the health of people actively exposed to smoking: Cuba, 2001

El consumo de tabaco es nocivo para la salud y puede ocasionar distintas enfermedades como el cáncer y afectaciones en la calidad de vida. El objetivo de este trabajo fue describir la calidad de vida relacionada con la salud de las personas expuestas activamente al tabaco, mediante el EuroQol-5D, tomando los datos de la II Encuesta Nacional de Factores de Riesgo de Enfermedades no Trasmisibles de Cuba en el 2001. Se calcularon números absolutos, porcentajes, odds ratio con su intervalo de confianza al 95 por ciento y se modeló con una regresión logística. Los porcentajes de individuos con calidad de vida relacionada con la salud óptima difieren significativamente entre expuestos y no expuestos de 66,78 a 58,93 por ciento y controlando la exposición también hubo diferencias entre estos porcentajes por sexo, edad y estado civil. Los odds ratio estimados en el modelo fueron 3,36, 1,02 y 3,60, respectivamente.

Smoking is harmful for health and may cause a number of diseases such as cancer and problems in quality of life. The objective of the present paper is to describe the quality of life related to the health of people actively exposed to smoking through EuroQol-5D, taking data from the Second National Survey of Risk Factors for Non-Communicable Diseases in Cuba in 2001. Absolute numbers, percentages, odd ratios with 95 percent confidence intervals were estimated and also logistic regression was modelled. The percentage of individuals with quality of life related to optimal health condition significantly differed between those exposed and those non-exposed to smoking (66,78 percent and 58,93 percent respectively) and controlling for exposure, there were still differences between these percentages according to sex, age and marital status. Estimated odd ratios for this model were 3,36, 1,02 and 3,60, respectively.

Hidradenitis suppurativa / Hidradenitis suppurativa

Introducción: la hidradenitis supurativa es una enfermedad inflamatoria, crónica, recurrente, originada en las glándulas apocrinas; se puede localizar en axila, ingle, periné, región anoperineal y cuero cabelludo. Objetivo: conocer la frecuencia y el manejo terapéutico. Material y métodos: se trató de un estudio retrospectivo, transversal y descriptivo, Se incluyeron los pacientes portadores de hidradenitis supurativa atendidos en la Unidad de Coloproctología del Servicio de Gastroenterología, Hospital General de México, de enero de 1995 a diciembre de 2004. Resultados: se revisaron 12,689 expedientes clínicos y se identificaron 15 pacientes (0.12 %), de los cuales nueve cumplieron los criterios de inclusión. Conclusiones: la hidradenitis supurativa es una enfermedad compleja que constituye un reto diagnóstico y terapéutico.

BACKGROUND: Hidradenitis suppurativa is an inflammatory, chronic and recurrent disease of appocrine glands, located in inguinal, axillar, perineal, perianal areas and scalp. OBJECTIVE: To determine the frequency and therapeutic management. METHODS: A retrospective, transverse, and descriptive study was carried out. All patients with hidradenitis suppurativa, and who were managed by the Coloproctology Unit from the Gastroenterology Service of the General Hospital of Mexico City from January 1995 to December 2004, were included. RESULTS: We reviewed 12,689 files and identified 15 patients (0.12%) with hidradenitis suppurativa; nine fulfilled inclusion criteria. CONCLUSIONS: This is a complex disease with a diagnostic and therapeutic challenge.