ABSTRACT
Nanotechnology is an emerging science which is associated with the synthesis of nanoparticles mediated with different chemical compositions in variable sizes and its application in health science for human benefit. We wanted to synthesize cinnamon oil mediated gold nanoparticles and evaluate their cytotoxicity using Brine Shrimp Lethality Assay. METHODS1 mL of cinnamon oil was dissolved in 9 mL of distilled water and kept in a beaker. 90 mL of 1 mM Gold chloride in distilled water was added, mixed and kept in an orbital shaker for nanoparticle synthesis. Color change was noted which indicated the presence of nanoparticles. Preparation of cinnamon oil mediated gold nanoparticles was confirmed using a UV-visible spectrophotometry and the size and shape of the nanoparticles was assessed using Scanning Electron Microscope. Varying concentrations of the synthesized gold nanoparticles was added in separate wells containing live brine shrimp (Artemia salina) and left undisturbed for 24 hours, after which the number of live shrimp larvae was counted in each well to assess the cytotoxicity. RESULTSCinnamon oil mediated gold nanoparticles were synthesized. Gold nanoparticles were characterized using Scanning Electron Microscope and were 50 nm in diameter. Brine Shrimp Lethality was done and the cytotoxicity of theses gold nanoparticles was found to be increasing with increasing concentration of the administered gold nanoparticles. CONCLUSIONSThe study concludes that evaluating the safety levels is of utmost importance prior to administration of nanoparticles for therapeutic and diagnostic purposes as there was a dose dependent cytotoxicity with cinnamon oil mediated gold nanoparticles.
ABSTRACT
This review aims to develop a systematic approach for the selection of suitable and safer dental materials available in the market thereby giving an insight to predict their risk of inducing allergic reactions. The review was conducted by referring to various relevant articles and retrospective studies.It was noted that the most common allergic reactions in dental staff are allergies to latex, acrylates and formaldehyde. The other dental materials that caused irritation included impression materials, metals, resins, rubber products, amalgam restorations. The study comprehended that dental personnel have high risk of allergy from latex gloves, bio aerosols inhaled during the dental practice, contact dermatitis and from the usage of various other potentially harmful dental materials. On the other hand, the patients have high risk of irritation from commonly used dental materials like metals (gold, mercury, chromium, palladium etc), root canal sealants, obturation materials, formaldehyde, amalgam restorations, resin, acrylates, impression materials, latex and local anesthesia. Patients begin to develop symptoms of stomatitis, burning, tingling, cheilitis, oral lichenoid lesions, lip and facial swelling. The oral cavity is exposed to various sensitizing substances that cause allergic reactions. Hence it is necessary to provide comprehensive dental treatment without compromising the health of the patient
ABSTRACT
Cassia oleoresin is an extract isolated from dried barks of Cinnamomum cassia Blume (family Lauracea). The plant has been reported to have anti-diabetic, anti-oxidant, anti-hypertriglyceridemic effect, mainly due to its phytochemical constituents such as phenolic and volatile compounds. Cinnamon also helps in arthritis, fibromyalgia and psoriasis. The aim of this study was to prepare magnesium oxide nanoparticles using Cassia oleoresin and to evaluate the cytotoxic effect on Brine shrimp. The magnesium oxide nanoparticle was prepared from magnesium chloride and Cassia oleoresin and was confirmed by UV- Visible Spectroscopy and morphology was confirmed by TEM. Brine shrimps lethality bioassay was carried out to investigate the cytotoxicity of Cassia oleoresin mediated magnesium oxide nanoparticles. Ten brine shrimp nauplii were placed in each well of the Eliza plate and filled with 5 μL ,10 μL ,15 μL ,20 μL ,25 μL of Cassia oleoresin mediated magnesium oxide nanoparticles After 24 hours of incubation, the wells were observed and the number of surviving brine shrimp nauplii were counted to assess the cytotoxicity. The UV -Visible spectroscopy showed a peak at 400 peak and TEM analysis showed a particle size of 70 nm. After 24 hours incubation of the brine shrimps in the nanoparticle solution, all 10 brine shrimps survived in 5μL and 10 μL concentrations. 3 brine shrimps nauplii survived in 15μL conc. 1 brine shrimp nauplii survived in 20μL and 25μL concentrations each. Within the limits of this study it can be concluded that at low concentrations the prepared nanoparticle was safe and may be used for biomedical application.
ABSTRACT
Objective: The objective was to compare the biochemical changes of amikacin by autoinjector delivery and manual injection in rats. Materials and Methods: Amikacin drug cartridge (500 mg/2 mL) for autoinjectors was diluted to 63 mg/mL and rats were administered 1.2 mL, i.p. One group was given 3 and a second group 7 injection on consecutive days. 3 and 7 days manual injection of same dose of amikacin (about 500 mg/kg, i.p.) and a control group (saline) were also included (total 5 groups). On day 4 or 8 biochemical parameters were studied. Results: Significant increase in urea, creatinine and aspartate aminotransferase were observed in 7 days administration in both autoinjector and manual injection groups compared to control group. All other parameters viz., glucose, cholesterol, total triglycerides, bilirubin, uric acid, total protein, albumin, alanine aminotransferase and alkaline phosphatase did not show any significant change. No significant change was observed in 3 days administration groups. Conclusion: High dose of amikacin for longer duration is known for its nephrotoxicity which is evidenced by the increase in urea and creatinine in both autoinjector and manual injection groups. This study shows that autoinjector device for amikacin which is new can be considered for further research work.