ABSTRACT
Orthodontically induced inflammatory root resorption [OIIRR] is considered to be an important sequel associated with orthodontic tooth movement [OTM]. OTM after Socket preservation enhances the periodontal condition before orthodontic space closure. The purpose of this study is to investigate the histologic effects of NanoBone[R], a new highly nonsintered porous nano-crystalline hydroxyapatite bone on root resorption following OTM. This experimental study was conducted on four male dogs. In each dog, four defects were created at the mesial aspects of the maxillary and mandibular first premolars. The defects were filled with NanoBone[R]. We used the NiTi closed coil for mesial movement of the first premolar tooth. When the experimental teeth moved approximately halfway into the defects, after two months, the animals were sacrificed and we harvested the area of interest. The first premolar root and adjacent tissues were histologically evaluated. The three-way ANOVA statistical test was used for comparison. The mean root resorption in the synthetic bone substitute group was 22.87 +/- 11.25×10[-4] mm[2] in the maxilla and 21.41 +/- 11.25×10[-4] mm[2] in the mandible. Statistically, there was no significant difference compared to the control group [p>0.05]. The use of a substitution graft in the nano particle has some positive effects in accessing healthy periodontal tissue following orthodontic procedures without significant influence on root resorption [RR]. Histological evaluation in the present study showed osteoblastic activity and remodeling environment of nanoparticles in NanoBone[R]
Subject(s)
Animals , Nanoparticles , Tooth Socket , Root Resorption , Tooth Movement Techniques , Orthodontics , Inflammation , DogsABSTRACT
Cleft lip with or without cleft palate [CL/P] is one of the most common congenital anomalies and the etiology of orofacial clefts is multifactorial. Transforming growth factor alpha [TGFA] is expressed at the medial edge epithelium of fusing palatal shelves during craniofacial development. In this study, the association of two important TGFA gene polymorphisms, BamHI [rs11466297] and RsaI [rs3732248], with CL/P was evaluated in an Iranian population. The frequencies of BamHI and RsaI variations were determined in 105 unrelated Iranian subjects with nonsyndromic CL/P and 218 control subjects using PCR and RFLP methods, and the results were compared with healthy controls. A p-value of <0.05 was considered statistically significant. The BamHI AC genotype was significantly higher [p=0.016] in the patients [12.4%] than the control group [5.0%]. The BamHI C allele was significantly higher [p=0.001; OR=3.4, 95% CI: 1.6-7.4] in the cases [8.0%] compared with the control group [2.5%]. Our study showed that there was an association between the TGFA BamHI variation and nonsyndromic CL/P in Iranian population
ABSTRACT
Statement of the Problem: A major objective of investigators is to clarify the role of metabolites in achievement of maximum tooth movement with minimal root damage during orthodontic tooth movement [OTM]
Purpose: The aim of this study was to determine the effect of administration of thyroid hormone, prostaglandin E2, and calcium on orthodontic tooth movement and root resorption in rats
Materials and Method: Sixty four male Wistar rats were randomly divided into 8 groups of eight rats each: 1- 20micro g/kg thyroxine was injected in traperitoneally after installation of the orthodontic appliance. 2- 0.1 ml of 1 mg/ml prostaglandin E2 was injected submucosally. 3- 10% [200 mg/kg] calcium gluconate was injected. 4- Prostaglandin E2 was injected submucosally and 10% calcium was injected intraperitoneally. 5- Thyroxine was injected intraperitoneally and prostaglandin E2 was injected submucosally. 6- 20micro g/kg thyroxine with calcium was injected. 7- Prostaglandin E2 was injected submucosally with calcium and thyroxine. 8- Distilled water was used in control group. The orthodontic appliances comprised of a NiTi closed coil were posteriorly connected to the right first molar and anteriorly to the upper right incisor. OTM was measured with a feeler gauge. The mid-mesial root of the first molar and the adjacent tissues were histologically evaluated. The Data were analyzed by one-way ANOVA and Student-Newman-Keuls test
Results: The highest mean OTM was observed in the thyroxine and prostaglandin E2 group [Mean +/- SD = 0.7375 +/- 0.1359 mm] that was significantly different [p< 0.05]. A significant difference [p< 0.05] in root resorption was observed between the prostaglandin E2 [0.0192 +/- 0.0198 mm[2]] and the other groups
Conclusion: It seems that the combination of thyroxine and prostaglandin E2, with a synergistic effect, would decrease the root resorption and increase the rate of orthodontic tooth movement in rats
ABSTRACT
Hydroxyapatite [Ca[10][PO][4][6][OH][2] is the major inorganic component of hard tissues, the best bio-active materials, which is compatible with the bone tissue. In addition, hydroxyapatite nanoparticles [Nano-HA] have received enormous national attention in medical and dental applications recently; but the ultimate fate of the Nano-HA within the body is still unknown. Degradation products of nanomaterials are potentially cytotoxic. Thus, it is essential to assess biocompatibility before their usage in clinical applications. Purpose of this research is to evaluate toxicity of hydroxyapatite nano particles on the human peripheral blood mononuclear cells. In this experimental study, nano sized, rod-like hydroxyapatite particles sterilizalied then HPBMCs were cultured on 96-well plate. Cells were exposed to Nano-HA at the following concentrations: 15.5, 32, 65, 125, 250, 500, 1000, 2000, 4000, 8000 ppm. Later, For measuring the cell vitality, MTT method was utilized .Measuring the photo absorption was done by ELISA READER system at 570 nm, which evaluated the vitality of cell by the value of MTT absorption cells. The statistical ANOVA test was used in this study. All of drug concentration were effective in Loweriy cellular biologic ectivity but none of them were statis fically significant. Therefore as a conclusion we can adjudicate that [Nano-HA] biomaterial is the material which is compatibility with the human blood mononuclear cells
ABSTRACT
Background and Aim: Antimicrobial effects of nano silver particles have received enormous attention in medical and dental applications. Despite of wide spread use of nanosilver there is not enough studies about its side effects on human. Some studies have exhibited cytotoxicity of nano-silver particles. This research was designed to investigate the cytotoxic effect of nano silver particles on L929 fibroblasts cells, by MTT assay
Materials and Methods: In this experimental study, nano silver particles with 5,10,20,30,40 and 50 ppm concentration were exposed to 10.000 L929 fibroblast cells then cell vitality was assessed after 2,24,48 and 72 hours by MTT method. The light absorption was evaluated by Elisa Reader and recorded. Data was subjected to ANOVA test for statistical analysis
Results: In all concentration groups, vitality of cells were the least after 24 hours, but the vitality of cells increased after 48 hours. The increase after 48H was statistically significant [p<0.05]. Also concentrations more than 20 ppm in 2,24 and 48 H were significantly cytotoxic for fibroblasts
Conclusion: Nano silver particles at concentrations less than 20 ppm after 72 hours did not have toxic effects
ABSTRACT
Background and Aim: Equipments and surfaces in dental clinics and centers are always in risk of contamination. When there is no possibility of sterilizing them, fast acting disinfecting agents are used. In this study, antibacterial effect of a new disinfecting solution, named Nanex,against streptococcus mutans and pseudomonas aeroginosa was compared with pure Nano silver solution and Deconex 53 plus
Materials and methods: In this experimental study, in order to determine the effective antibacterial concentration , serial dilution technique ,evaluation of [MIC] and [MBC] were used. Finally bacterial growth was assessed
Results: The # 4 solution [containing 100 ppm Nano silver and 2% Deconex] showed the least MIC and MBC for streptococcus mutans. The # 7 solution [containing 133 ppm Nano silver and 1% Deconex] showed the least MIC and MBC for psuedomonas aerogenosa
Conclusion: Thus, antibacterial effect of Nanex solution was more potent and effective than pure solution of nano silver and deconex