ABSTRACT
Professor DU Xiao-shan 's academic thought and clinical experiences was introduced in this paper. He has noble medical ethics and is fair to the patients without distinction. He has great learning and rich practical experiences, and he specializes in all kinds of common diseases as well as many difficult and complicated cases. He never stops studying the ancients to make innovation and he creates the technique of the quick-puncture and slow-twist and DU's heat-reinforce method. He teaches to carry forward acupuncture and moxibustion and his achievements have become an important part of acupuncture and moxibustion science.
Subject(s)
Humans , Acupuncture , Education , History , Acupuncture Therapy , History , Methods , China , History, 20th Century , History, 21st CenturyABSTRACT
<p><b>AIM</b>To prepare lung targeting azithromycin cationic liposomes and to observe its tissue distribution in mice.</p><p><b>METHODS</b>The azithromycin cationic liposomes were prepared by thin film method with freeze-thawing steps. HPLC method was established and validated for the determination of azithromycin in tissues of mice.</p><p><b>RESULTS</b>The particle size of the liposomes was 6.582 microm with zeta potential of +19.5 mV. The entrapment efficiency was more than 75%. The liposomes was stable in 6 months stored at 4 degrees C. The release in vitro was characterized by Higuchi equation. Azithromycin liposomes and free azithromycin solution were injected intravenously at a dose of 80 mg x kg(-1) to mice. Compared with solution, liposomes were characterized by slower clearance, increased half-life and the AUC increased by 7.4 fold in lung.</p><p><b>CONCLUSION</b>Thin film method with freeze-thawing steps could increase the entrapment efficiency and increase the particle size of azithromycin liposomes. After modification of lipid membrane with stearylamine, the cationic liposomes were prepared. The azithromycin concentration and AUC increased in lung after iv administration to mice of the cationic liposomes. This offered a good information for preparing liposomes targeting on the lung.</p>