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Objective To explore the effects of Levamlodipine on viability,proliferation and apoptosis of neural stem cells after hypoxia-ischemia injury in adult rats. Methods Hypoxic-ischemic damage to adult neural stem cells was simulated in the established oxygen/glucose deprivation (OGD)models.Four groups were designed according to Levamlodipine concentrations:0 μmol/L,0.5 μmol/L,1.0 μ mol/L and 5.0 μmol/L. Effects of Levamlodipine at 4 different concentrations on the viability,proliferation and apoptosis of hippocampal neural stem cells after OGD were tested by CCK-8 assay,Edu fluorescence staining and flow cytometry (Annexin V-FITC/PI),respectively. Results Models of hypoxia-ischemia damage to hippocampus neural stem cells were successfully established by OGD for 6hours.Compared with control group (0 μmol/L),the viability of hippocampal neural stem cells was significantly increased in the other 3 groups (0.5 μmol/L,1.0 μmol/L and 5.0 μmol/L) (P＜0.05).The proportion of proliferating cells after OGD was significantly increased at S phase in 5.0 μmol/LLevamlodipine group (P＜0.05).The proportion of apoptotic cells after OGD was significantly decreased in 1.0 μ,mol/L and 5.0μ mol/L Levamlodipine groups (P＜0.05). Conclusion Levamlodipine may protect neural stem cells from hypoxic-ischemic injury in adult rats.
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Objective To evaluate the therapeutic effect of Shuxuetong injection on acute ischemic stroke. Methods One hundred and ninety-six patients with acute ischemic stroke were randomly divided into Shuxuetong injection treatment group (n=98) and control group (n=98). The neurological deficiency scores, scores of cognitive dysfunction and clinical outcomes were evaluated; and the cerebral blood flow were evaluated by Doppler before treatment and 2 weeks after the therapy.Results The improvement of neurological function and clinical outcomes in the Shuxuetong injection treatment group was more obvious as compared with that in the control group and the improvement rate of patients in the treatment group was higher than that in the controls; Patients in the Shuxuetong injection treatment group enjoyed less worsening than the controls. The cognitive dysfunction 2 weeks after the therapy were significantly improved as compared with those before treatment (P＜0.05); and the cerebral blood flow after the treatment was significantly increased as compared with that before the treatment (P＜0.05); these improvements in the Shuxuetong injection treatment group were much obvious as compared with those in the controls. Conclusion Shuxuetong is effective in treating cerebral ischemic patients.
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Objective To establish simple,stable and reliable rat models of oxygen glucose deprivation/reoxgenation(OGD/R)in adult neural stem cells(NSCs)in vitro.Methods The NSCs from adult Fisher344 rats were cultured in serum-free medium and identified using nestin and DAPI immunofluorescent double staining.These cells were washed with a Earle′s balanced salt solution without glucose for 2 times,then,incubated for different periods(2,4,6,8 and 10 h)in a trigas incubator with an atmosphere of 1％ O2,5％CO2 and 94％ N2,98％ humidity at 37 ° C.And then,these cells were removed from the anaerobic incubator,washed,and added DEME/F12 containing bFGF supplement.A normoxic-normoglycemic control group was employed.Morphological assessment of NSCs was performed by light microscopy after re-oxgenation for 24 h; CCK-8 colorimetric method was used to determine the survival and proliferation of NSCs,and flow cytometry was employed to detect the apoptosis of NSCs.Results After the setting of oxygen glucose deprivation for 2 h,the OD value and the survival rate in the OGD cells were increased as compared with those in control group without significant difference(P＞0.05).While the morphological damage of NSCs aggravated gradually and the OD value decreased in OGD cells following the prolongation of times; under the setting of oxygen glucose deprivation for 6 h,the OD value in OGD cells obviously decreased as compared with that in the control group(P＜0.05); under the setting of oxygen glucose deprivation for 6 h,the survival rate obviously decreased and the apoptosis rate significantly increased in OGD cells as compared with that in the control group(P＜0.05); under the setting of oxygen glucose deprivation for 6 h,the apoptosis rate of NSCs excessed to 50％.Conclusion By means oftrigas incubator,simple,stable and reliable models of OGD/R in NSCs in vitro can be successfully established.
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<p><b>OBJECTIVE</b>To report a case of critical illness polyneuropathy (CIP) with Parkinson disease and discuss the development, clinical features and early diagnosis of this condition.</p><p><b>METHODS</b>The clinical data of a patient with CIP and Parkinson's disease and the relevant literature were reviewed.</p><p><b>RESULTS</b>This case showed no typical disease course of sepsis, and the condition exacerbated rapidly. The patient presented initially with abnormal homeostasis, followed by rapid onset of respiratory muscle weakness to require mechanical ventilation, but no limb weaknesses were detected. Intravenous antibiotics and aggressive treatment of sepsis did not produce any positive responses to wean from mechanical ventilation. Examinations of creatine kinase and cerebrospinal fluid showed no abnormalities. Electromyography and nerve conduction studies demonstrated declined nerve conduction velocity and decreased sensory and motor muscle action potentials, suggesting the possibility of CIP.</p><p><b>CONCLUSION</b>In patients with Parkinson disease, the occurrence of sepsis with prolonged mechanical ventilation and limb weakness indicates the necessity of neurophysiological examination, muscle biopsies and laboratory tests, which may help detect CIP in the early phase. Proper interventions of sepsis may reduce the likeliness of CIP. Elimination of the risk factors and aggressive management of sepsis can be effective measures for preventing CIP.</p>
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Aged , Humans , Male , Parkinson Disease , Polyneuropathies , Diagnosis , Respiration, Artificial , Respiratory Insufficiency , SepsisABSTRACT
<p><b>OBJECTIVE</b>To investigate the association between the heat shock protein 70-2 gene polymorphism and ankylosing spondylitis (AS).</p><p><b>METHODS</b>The polymorphisms of HSP70-2 gene Pst I 1267 site were analysed in 176 Chinese Han AS patients and 127 healthy controls by PCR and restriction fragment length polymorphisms(RFLP) methods.</p><p><b>RESULTS</b>In AS patients HSP70-2 genotypes AA, AG and GG were 46.6%, 46.0% and 7.4% respectively, frequencies of A and G were 69.6%(A) and 30.4%(G). In healthy controls HSP70-2 genotypes AA, AG and GG were 44.1%, 48.8% and 6.9% respectively, frequencies of A and G were 68.5%(A)and 31.5%(G). No significant differences were found in the distribution of HSP70-2 genotypes and allele frequencies between AS patients and controls.</p><p><b>CONCLUSION</b>Our results indicate that the there may be no association of the HSP70-2 gene polymorphism with AS in Chinese Han population.</p>