ABSTRACT
<p><b>OBJECTIVE</b>To observe the histological and immunohistochemical characteristics of the pulp of teeth after pulpotomy.</p><p><b>METHODS</b>Twenty-nine permanent anterior teeth with completely formed roots after pulpotomy due to dental trauma were selected. Thirty permanent premolars with completely formed roots served as control, which were extracted for orthodontic treatment. HE stain and immunohistochemical study of collagen I and collagen III were performed on the root pulp of these two groups.</p><p><b>RESULTS</b>There were degenerative changes in root pulp of the teeth after pulpotomy, such as vacuolization and homogenization of the odontoblasts, cell reduction, fibrosis, hyaline degeneration and calcification. In healthy root pulp, collagen I had a dispersed distribution, calcification substance was stained positive, but collagen III weakly stained in the extreme at peripheric pulp, and calcification substance stained negative. While in pulp of teeth after pulpotomy, both types of collagens had increased expression, fibers aggregated forming thick fiber bundles. In the wall of blood vessels collagen I had increased expression, but collagen III decreased.</p><p><b>CONCLUSIONS</b>The root pulp below dentin bridge after pulpotomy was different from the healthy pulp, and there were some histological degenerative changes in the pulp of the immature anterior teeth after pulpotomy. It is suggested that root canal treatment should eventually be performed on these teeth.</p>
Subject(s)
Child , Female , Humans , Male , Collagen , Metabolism , Dental Pulp , Metabolism , Pathology , Immunohistochemistry , Pulpotomy , Staining and LabelingABSTRACT
<p><b>OBJECTIVE</b>To observe the outcome of the fractured immature anterior permanent teeth after pulpotomy by X-ray measurement.</p><p><b>METHODS</b>A total of 31 (25 cases) fractured immature anterior permanent teeth after pulpotomy was reviewed. Radiographic measurements were performed on 12 cases with unilateral fractured central incisors and completely formed roots after pulpotomy and 100 (50 subjects) healthy maxillary central incisors.</p><p><b>RESULTS</b>The roots development continued after pulpotomy, and the root length and periapical configuration were both similar to the healthy teeth, but some root canals became narrow or even nearly obturated at the root apex. There was no significant difference in root canal width between healthy right and left maxillary central incisors at the site of 1/4 [right: (0.67 +/- 0.14) mm, left: (0.66 +/- 0.12) mm, P > 0.05], 1/2 [right: (1.05 +/- 0.19) mm, left: (1.04 +/- 0.17) mm, P > 0.05], 3/4 [right: (1.78 +/- 0.34) mm, left: (1.76 +/- 0.30) mm, P > 0.05] of root, but the root canal width of the fractured incisors at the same site was significantly different from that of the contralateral healthy incisor, the 1/4 site pulpotomy: (0.70 +/- 0.23) mm, control: (0.99 +/- 0.17) mm, the 1/2 site pulpotomy: (1.16 +/- 0.24) mm, control: (1.51 +/- 0.25) mm; the 3/4 site pulpotomy: (1.96 +/- 0.34) mm, control: (2.24 +/- 0.36) mm.</p><p><b>CONCLUSIONS</b>There was a tendency that the root canals of fractured teeth after pulpotomy became narrowed and obturated. It should not be followed up for too long to perform root canal treatment when roots completely formed.</p>