ABSTRACT
Multidrug resistance (MDR) is one of the major obstacles in cancer chemotherapy. Our previous study has shown that icariin could reverse MDR in MG-63 doxorubicin-resistant (MG-63/DOX) cells. It is reported that icariin is usually metabolized to icariside II and icaritin. Herein, we investigated the effects of icariin, icariside II, and icaritin (ICT) on reversing MDR in MG-63/DOX cells. Among these compounds, ICT exhibited strongest effect and showed no obvious cytotoxicity effect on both MG-63 and MG-63/DOX cells ranging from 1 to 10 μmol·L. Furthermore, ICT increased accumulation of rhodamine 123 and 6-carboxyfluorescein diacetate and enhanced DOX-induced apoptosis in MG-63/DOX cells in a dose-dependent manner. Further studies demonstrated that ICT decreased the mRNA and protein levels of multidrug resistance protein 1 (MDR1) and multidrug resistance-associated protein 1 (MRP1). We also verified that blockade of STAT3 phosphorylation was involved in the reversal effect of multidrug resistance in MG-63/DOX cells. Taken together, these results indicated that ICT may be a potential candidate in chemotherapy for osteosarcoma.
Subject(s)
Humans , ATP Binding Cassette Transporter, Subfamily B , Genetics , Metabolism , Antineoplastic Agents , Pharmacology , Apoptosis , Cell Line, Tumor , Cell Survival , Dose-Response Relationship, Drug , Doxorubicin , Metabolism , Pharmacology , Toxicity , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Flavonoids , Pharmacology , Gene Expression Regulation, Neoplastic , Multidrug Resistance-Associated Proteins , Genetics , Metabolism , Osteosarcoma , Drug Therapy , Metabolism , Pathology , Phosphorylation , Rhodamine 123 , Metabolism , STAT3 Transcription Factor , Metabolism , Triterpenes , PharmacologyABSTRACT
Multidrug resistance (MDR) is one of the major obstacles in cancer chemotherapy. Our previous study has shown that icariin could reverse MDR in MG-63 doxorubicin-resistant (MG-63/DOX) cells. It is reported that icariin is usually metabolized to icariside II and icaritin. Herein, we investigated the effects of icariin, icariside II, and icaritin (ICT) on reversing MDR in MG-63/DOX cells. Among these compounds, ICT exhibited strongest effect and showed no obvious cytotoxicity effect on both MG-63 and MG-63/DOX cells ranging from 1 to 10 μmol·L. Furthermore, ICT increased accumulation of rhodamine 123 and 6-carboxyfluorescein diacetate and enhanced DOX-induced apoptosis in MG-63/DOX cells in a dose-dependent manner. Further studies demonstrated that ICT decreased the mRNA and protein levels of multidrug resistance protein 1 (MDR1) and multidrug resistance-associated protein 1 (MRP1). We also verified that blockade of STAT3 phosphorylation was involved in the reversal effect of multidrug resistance in MG-63/DOX cells. Taken together, these results indicated that ICT may be a potential candidate in chemotherapy for osteosarcoma.
Subject(s)
Humans , ATP Binding Cassette Transporter, Subfamily B , Genetics , Metabolism , Antineoplastic Agents , Pharmacology , Apoptosis , Cell Line, Tumor , Cell Survival , Dose-Response Relationship, Drug , Doxorubicin , Metabolism , Pharmacology , Toxicity , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Flavonoids , Pharmacology , Gene Expression Regulation, Neoplastic , Multidrug Resistance-Associated Proteins , Genetics , Metabolism , Osteosarcoma , Drug Therapy , Metabolism , Pathology , Phosphorylation , Rhodamine 123 , Metabolism , STAT3 Transcription Factor , Metabolism , Triterpenes , PharmacologyABSTRACT
Pancreatic cancer is the seventh most common cause of cancer deaths worldwide.Although more and more progress of oncotherapy has been achieved in recent years,very few progress has been achieved in pancreatic cancer.This review summarizes the molecular signaling pathway and relevant targeting drug,their recent clinical and experimental findings of this disease.It also presents the situation and future development of targeting treatment in pancreatic cancer.
ABSTRACT
Objective: Through orthogonal test to find out the scientific conditions for dry-heat sterilization process of Scutellaria Baicalensis Radix powder. Methods: The microbial (bacterium and molds) quantity and the content of baicalin as the indexes, the temperature, time, thickness of powder as factors, the sterilization process for Scutellaria Baicalensis Radix powder was determined by L9(34) orthogonal test. Results: The optimal technological conditions for sterilization were 100°C, 10 h, and the powder thickness of 2 cm. Conclusion: This condition is proved by the amplified test and the large scale production, after the sterilization, the hygiene indexes of Scutellaria Baicalensis Radix powder can reach the requirements of Chinese Pharmacopoeia 2010. There is a good reproducibility but no significant difference for the content of baicalin before and after the sterilization.
ABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Celecoxib, one of the new generation of non-steroidal anti-inflammatory drugs (NSAIDs), has a specific inhibitory effect on COX-2. Studies have shown that celecoxib can inhibit the proliferation of tumor cells and induce cell apoptosis, which has been confirmed in colorectal tumors and familial adenomatous polyposis. This study explored the effect of celecoxib on the proliferation and apoptosis of human glioma cell line U251 and elucidated the correlation between the effect of celecoxib and the expression of survivin.</p><p><b>METHODS</b>U251 cells were treated with different concentrations of celecoxib. Cell morphologic changes were observed by optical microscopy. MTT assay was used to detect the absorbance value and to calculate inhibition and survival rates. The rates of apoptosis of U251 cells after 48 h of treatment with celecoxib were assessed by flow cytometry. The expression of survivin was analyzed by immunocytochemistry (ICC) and Western blot analysis. The expression of survivin mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Significant morphologic changes were shown in U251 cells after treatment with celecoxib. The MTT assay results revealed that celecoxib inhibited the proliferation of U251 cells and the inhibitory rates significantly increased in a dose-and time-dependent manner. After 48 h of treatment with celecoxib, the apoptotic cells could be obviously observed, and the apoptosis rate significantly increased with increases in concentrations of celecoxib. The expression of survivin was observed in the control group, however, the expression of survivin was significantly down-regulated as the concentration of celecoxib increased. The level of survivin mRNA expression in U251 cells was significantly down-regulated after treatment with different concentrations of celecoxib (P < 0.05).</p><p><b>CONCLUSIONS</b>The inhibition of proliferation and apoptosis in U251 cells could be induced by celecoxib in a dose-and time-dependent manner, and its mechanism might be the downregulation of the expression of survivin.</p>
Subject(s)
Humans , Apoptosis , Brain Neoplasms , Metabolism , Pathology , Celecoxib , Cell Line, Tumor , Cell Proliferation , Cyclooxygenase Inhibitors , Pharmacology , Dose-Response Relationship, Drug , Down-Regulation , Glioma , Metabolism , Pathology , Inhibitor of Apoptosis Proteins , Genetics , Metabolism , Pyrazoles , Pharmacology , RNA, Messenger , Metabolism , Sulfonamides , PharmacologyABSTRACT
Objective: To analyze the effect of three therapeutic methods to find an optimal approach to the treatment of intracranial bacterial infection by retrospectively reviewing 33 intracranial bacterial infection patients who were admitted from 1995 to 2008 in our hospital. Methods: The treatments by intermittent lumbar puncture, continuous lumbar subarachnoid space drainage, and embedment of Ommaya cyst for continuous drainage from the ventricles were performed in 15 cases, 12 cases, and 6 cases respectively along with intravenous application of full dose of antibiotics. Results: Nineteen cases were cured and the best prognosis was from the group of Ommaya cyst embedment and continuous drainage from the ventricles. Conclusion: Management goals are prompt recognition of the central nervous system (CNS) infection, rapid identification of causative organisms and initiation of treatment with the optimal management methods for complications. Embedment of Ommaya cyst for continuous drainage from the ventricle is a safe and effective treatment for intracranial bacterial infection.
ABSTRACT
<p><b>AIM</b>To investigate the chemical constituents from Picria fel-terrae Lour.</p><p><b>METHODS</b>Column chromatography techniques were used to isolate the chemical constituents, physico-chemical constants and spectroscopic analysis were employed for structural elucidation. Results Two triterpenoids named picfeltarraenone I (1) and picfeltarraenin XI (2) were isolated, and their structures were established to be 3,11,22-trioxo-16alpha-hydroxy-(20S,24)-epoxy-cucurbit-5,23-diene (1) and 3,11,22-trioxo-16alpha-hydroxy-(20S,24)-epoxy-cucurbit-5, 23-diene-2beta-O-beta-D-glucopyranoside (2), respectively.</p><p><b>CONCLUSION</b>Compound 2 is a new compound, the 13CNMR data of compound 1 is reported for the first</p>
Subject(s)
Glucosides , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Scrophulariaceae , Chemistry , Triterpenes , ChemistryABSTRACT
Objective To study the clinical characteristics and curative effects of pancreatic cystade- nocarcinoma in order to improve its diagnostic and therapeutic accuracy.Methods A retrospective analysis was done on the clinical materials of 13 cases of pancreatic cystadenocarcinoma hospitalized in Shanxi Cancer Hospital from 1990 to 2006.Results The preoperative diagnosis were as follows:pancreatic cystadenocarci- noma 6 cases,pancreatic cystadenoma 2 cases,pancreatic cancer 1 case,pancreatic pseudocyst 4 cases.The misdiagnosis rate was 53.8 %.Surgical operation was done on the 13 cases,and 10 of them were treated by radical operation.A 5-year follow-up was done on 6 still alive cases,and 1 of them lived over 11 years.3 cases were treated by palliative operation,and all of them died within 3 years.Conclusion Since there is no specific clinical manifestations of pancreatic cystadenocarcinoma,it is very difficult to get an accurate preop- erative diagnosis.Radical operation is the most effective therapeutic methods.