Gastric intubation guided by angiographic guide wire in the treatment of coma patients / 现代临床护理

Objective To explore the effect of gastric intubation guided by angiographic guide wire on coma patients. Methods Forty-eight coma patients were randomly divided into the observation group and the control group with 24 cases in each group: conventional intubation and gastric intubation guided by angiographic guide wire were used respectively.The two groups were compared in terms of the success rate of one-time intubation.Result The one-time success rate in the observation group was signfiantly higher than that in the control group(95.8%vs.66.6%,P<0.05).Conclusion Intubation guided by angiographic guide wire may help to improve the success rate of one-time intubation and relieve the pains.

Gene expression of methyl-CpG-binding protein 2 and methyl-CpG-binding domain 2 in patients with systemic lupus erythematosus / 中华皮肤科杂志

Objective To investigate the gene expression of methyl-CpG-binding protein 2 (MeCP2) and methyl-CpG-binding domain 2 (mbd2) in patients with systemic lupus erythematosus(SLE) and their significance in the pathogenesis of SLE. Methods Semiquantitative reverse transcriptase-PCR was applied to detect the mRNA expression of MeCP2 and mbd2 in PBMCs obtained from relieved (n=17), active (n=17) SLE patients and healthy controls (n=17). The correlations were further analyzed among these parameters. Results No significant difference was observed in the expression level of MeCP2 mRNA among active SLE patients, relieved SLE patients and healthy controls (P>0.05). The expression of mbd2 in relieved SLE patients was significantly higher than that in health controls (t=12.8, P<0.01), but lower than that in active SLE patients (t=20.0, P<0.01). The expression of mbd2 positively correlated with SLEDAI (r=0.737, P=0.0001) of patients with SLE, and a positive correlation was also observed between the expression of mbd2 and MeCP2 in healthy controls (r=0.550, P=0.0222). Conclusions The expression of MeCP2 and mbd2 may be mutually constrained in normal human, but this relationship seems to be disturbed in patients with SLE.

Optimization of bacterial cellulose fermentation medium and observation of bacterial cellulose ultra-micro-structure / 生物工程学报

In order to improve the yield of bacterial cellulose (BC), the fermentation medium of BC-producing strain J2 (Gluconobacter) was optimized, and BC ultra-micro-structure was observed. Initially, Plackett-Burman design was employed to evaluate eight variables which were relevant to BC production. Three statistically significant parameters including yeast extract, ZnSO4, ethanol were selected and other 5 variables were not significant (P > 0.05). The optimized levels of three variables were defined by Box-Behnken design and response surface methodology (RSM). BC ultra-micro-structure was observed by scanning electron microscope (SEM) with cotton cellulose as comparison. The results indicated that the BC yield under the optimum fermentation medium was 11.52 g/100 mL, which was as 1.35 times as that under the original fermentation medium. The SEM photos manifested that bacterial cellulose ribbon, with a diameter less than 0.1 microm, was less than cotton cellulose ribbon. The bacteria inside the cellulose net were eliminated after the NaOH treatment.