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Objective To analyze the distributional characteristics of NPC1L1 1 735 C>G polymorphism and the relation-ship between serum lipid levels and obesity in Guangxi Han population.Methods Genotyping of the NPC1L1 1 735 C>G poly-morphism of 409 cases of Han was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis in all subjects.The relationship of different genotypes in blood lipid levels and obesity were evaluated.Re-sults The frequency of CC,CG and GG genotypes was 39.40%,46.00% and 14.60% in Han.The frequency of C and G alleles was 62.35% and 37.65% in Han.The levels of BMI,LDL-C,ApoB was different among the three genotypes (P G polymorphism in NPC1L1 gene may be correla-ted with serum lipid profiles and obesity,the G allele might increase the risk of hyperlipemia and obesity.
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The levels of total cholesterol, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, apolipoprotein (APO) A1 and APO B were lower in Bai Ku Yao than those in Han nationalities (all P<0.01). There was no significant difference in serum triglyceride levels and the ratio of Apo A1 to Apo B between two nationalities. Dyslipidemia was positively correlated with body mass index, waist circumference, total energy and total fat intakes, and inversely correlated with degree of physical activity and total dietary fiber intake in both ethnic groups. In addition, dyslipidemia was also positively correlated with age and alcohol consumption in Han, but not in Bai Ku Yao.
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Objective To study the effect of Tongxinluo on the plasma C-reactive protein(CRP)and endothelin-1(ET-1)in acute coronary syndrome(ACS) patients.Methods 100 patients with ACS were randomly divided into conventional therapy group and treatment group(conventional therapy+Tongxinluo gelatin capsule).The changes of CRP and ET-1 in the first day,7th and 14th day were observed.Results In the treatment group,CRP and ET-1 were significantly decreased in the 7th and 14th day(P＜0.05,P＜0.01),and there was significant decrease only in the 14th day(P＜0.05)in the conventional therapy group.CRP and ET-1 levels in the treatment group were significantly different as compared with conventional thereapy group(P＜0.01).Conclusion Tongxinluo capsule may protect blood vessel endothelium through inhibiting CRP and ET-1 to decrease the inflammatory response of endangium.
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Objective To detect the serum lipids levels in the subjects aged 40 years and over in the Guangxi Bai Ku Yao population. Methods A total of 485 subjects of Bai Ku Yao and 501 Han people aged 40 years and over were surveyed by cluster sampling methods. Informations on demography, diet and lifestyle were collected by standard questionnaires. Blood pressure, height,weight, waist circumference, serum lipids and apolipoproteins (apo) were measured, and body mass index (BMI) was calculated. Results Education level, height, weight, BMI, waist circumference,blood pressure levels, hypertensive prevalence, and the intakes of total energy, total fat, total protein, dietary cholesterol, and salt were higher in Han than in Bai Ku Yao (P<0.05 or P<0.01),whereas physical activity level, and the intakes of carbohydrate, vegetal protein, and total dietary fiber were higher in Bai Ku Yao than in Han (all P<0.001). The serum levels of total cholesterol,high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, apo A1, and apo B were significantly lower in Bai Ku Yao than in Han [(4. 43±0. 90)vs. (4.96±1.04)mmol/L, P<0. 001;(1.73±0. 42) vs. (2.01±0. 49) mmol/L, P<0. 001;(2. 62±0. 75) vs. (2. 72±0. 78)mmol/L, P<0. 05;(1. 36±0. 33) vs. (1. 48±0. 24)g/L, P<0. 001;and (0. 86±0.23) vs. (0. 95±0. 22)g/L,P<0. 001 ;respectively]. There were no significant differences in serum triglyceride level and the ratio of apo Al over apo B between the two ethnic groups (P>0. 05). Conclusions There are significant differences in lipids levels and the risk factors between Bai Ku Yao and Han populations, which might result from different dietary habits, life styles, and physical activities.
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Objective:To investigate cytokine(TNF-?,IL-1) effect on apoptosis and express of related protein in thyrocytes and the relationship with pathogenesis in Graves' disease(GD).Methods:To test thyroid tissues from 50 patients with GD Fas expression by immunohistochemical method.Thyroid tissues were collected from samples of GD operation and were cultured by primary culture method.Content of sFas in culture medium was tested by ELISA.Fas/sFas mRNA were tested by RT-PCR.Cytokine inducing apoptosis in Graves' disease showes observation group,normal people showes control group.Results:Compare apoptosis culture rate there is significant difference(P
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Objective To investigate the prevalence rate of hyperlipidemia in the middle-aged and elderly in Guangxi Hei Yi Zhuang population. Methods A total of 657 people of Hei Yi Zhuang nationality aged 40 years and over were surveyed. Blood pressure, body height, body weight, serum lipid and apolipoprotein levels were measured, and both body surface areas and body mass index were calculated. The results were compared with those in 520 people of Han nationality who also live in that district. Results The prevalence of hypercholesterolemia, hypertriglyceridemia, and hyperlipidemia in Hei Yi Zhuang vs. Han population were 28.9% vs. 35.8%(P0.05), and 36.2% vs. 42.3%(P0.05) respectively. Conclusions Prevalence of hyperlipidemia of Hei Yi Zhuang is lower than that of Han ethnic group, which might results from different dietary habit, life style, physical activity, and even genetic background.
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Objective To determine the effect of intravenous administration of vascular endothelial growth factor (VEGF) on myocyte apoptosis and the expression of P 53 , Fas, Bax and Bcl 2 in an experimental model rat with acute myocardial infarction Methods Thirty three male Sprague Dawley rats were subjected to left coronary artery ligation The rats were randomized to receive VEGF 165 heparin(treated group) or heparin saline(control group), respectively, 24 hours after surgery VEGF 165 (2 ?g/1 ml)with heparin(50 U)or heparin saline(50 U/1 ml)was administered daily via tail vein for 7 days(treated group, n=8;control group, n =10) and 14 days(treated group, n=7;control group,n =8)separately Apoptotic index and the expression of the P 53 , Fas, Bax and Bcl 2 in myocardium were measured at 9 or 16 days after coronary artery ligation Results The number of apoptotic myocytes in VEGF treated group was less than that in control group\[(10?2)% vs (28?2)%, P
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AIM and METHODS: To study the changes of serum vascular endothelial growth factor (VEGF) levels in a rat model of acute myocardial infarction (MI) and its significance. Eighty-eight male Sprague-Dawley rats were used in this study. MI was produced by left coronary arterial ligation in 80 animals, and eight rats undergoing thoracotomy but not coronary ligation served as controls (sham).Blood samples were drawn from the right atrium before (sham animals) and 1, 3, 6, 12, 24 hours and 2, 3, 5, 7, 14 days after MI( n =8,respectively). Serum VEGF concentrations were measured by a sensitive enzyme-linked immunosorbent assay with a rabbit polyclonal antibody specific for VEGF. RESULTS: In 8 sham animals, the concentration of serum VEGF was (66 99?17 83) pg/mL. Six hours after MI, the level of serum VEGF significantly increased to (125 68?28 07)pg/mL ( P