Differential expression of miRNAs in myocardial tissues of rats with lipopolysaccharide- induced endotoxemia / 南方医科大学学报

<p><b>OBJECTIVE</b>To analyze the differential miRNA expression profile in the myocardium of rats with lipopolysaccharide (LPS)-induced endotoxemia and explore the role of miRNA in endotoxin-induced myocardial injury.</p><p><b>METHODS</b>Twenty male SD rats received intraperitoneal injection of 10 mg/kg LPS (n=10) or an equivalent amount of saline solution (n=10). At 24 h after LPS injection, the rats were sacrificed to detect myocardial expressions of TLR4, TNF-α and IL-1β using real-time PCR and for observing myocardial ultrastructures under transmission electron microscopy. The differentially expressed miRNA in the myocardium were detected using a miRNA array, and the common differentially expressed miRNAs were selected for verifying their actual expressions using real-time PCR.</p><p><b>RESULTS</b>TLR4, TNF-α and IL-1β were over-activated in the myocardium of LPS-treated rats, in which mitochondria swelling, structural damaged and cytoplasmic vacuoles were observed. In LPS-challenged rats, miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, and miR-877 were found up-regulated significantly, whereas miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, and miR-3585-5p down-regulated in the myocardium.</p><p><b>CONCLUSION</b>Significant differential expression of the miRNAs occurs in the myocardium of LPS-treated rats, suggesting their involvement in endotoxin-induced myocardial injury.</p>

Establishment of an induced pluripotent stem cell line from a patient with acute lung injury / 南方医科大学学报

<p><b>OBJECTIVE</b>To establish an induced pluripotent stem (iPS) cell line from the skin fibroblasts of a patient with acute lung injury (ALI-iPS).</p><p><b>METHODS</b>We obtained iPS cells from a female ALI patient by forced expression of a cocktail of transcription factors (Sox2, Klf4, Oct4 and Nanog) into the dermal fibroblasts to result in reprogramming to a pluripotent state. The ALI-iPS cells generated were analyzed for colony morphology, alkaline phosphatase (AP) activity, surface antigens, and differentiation ability.</p><p><b>RESULTS</b>The ALI-iPS cells exhibited morphological and growth characteristics of ES cells, showed positivity for alkaline phosphatase by histochemical staining, and expressed ES cell marker genes. Subcutaneous injection of iPS cells into immunodeficient (SCID) mice resulted in tumors containing a variety of tissues from all the 3 germ layers.</p><p><b>CONCLUSION</b>The ALI patient-specific iPS cell line was successfully established to serve as a valuable model to study the cellular pathology of acute lung injury and develop high-throughput drug screening assays.</p>

The invasion ability and expressions of matrix metalloproteinase-13 and calcium-binding protein S100A4 are inhibited by hyperthermia in human Tca8113 cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of hyperthermia on anti-invasion of Tca8113 and the expression change of matrix metalloproteinase-13 (MMP-13) and calcium-binding protein S100A4 (S100A4).</p><p><b>METHODS</b>Tca8113 cell pools were incubated at 43 degrees C for 0, 40, 80, 120 min, respectively, and at 37, 41, 43, 45 degrees C respectively for 80 min. The effect of high temperatures on the invasion ability of Tca8113 was measured in vitro. The slides of cells were made and incubated at 43 degrees C for 0, 40, 80, 120 min, respectively. Immunocytochemical method was employed for detecting the expression change of MMP-13 and S100A4 protein. Tca8113 cells were incubated at 43 degrees C for 0, 40, 80, 120 min respectively and at 37, 41, 43, 45 degrees C respectively for 80 min. Western blot method was conducted for detecting the expressionchange of MMP-13 and S100A4 protein.</p><p><b>RESULTS</b>As incubating time at higher temperature lasted, the proportion of the cells with invasion ability decreased. Except groups of 40 min and 80 min at 43 degrees C and 41, 43 degrees C for 80 min, the rest groups show significant statistic differences (P < 0.05). The expression intensity of MMP-13 and S100A4 proteins in Tca8113 cells would decrease as incubating time at higher temperature lasted. The content of MMP-13 and S100A4 proteins would decrease as incubating time at higher temperature lasted or incubating temperature increased. Except the groups of 40, 80 min at 43 degrees C and 41, 43 degrees C for 80 min, statistic differences were identified (P < 0.05).</p><p><b>CONCLUSION</b>The invasion of Tca8113 could be inhibited by hyperthermia. The mechanism of this effect may be due to protein expression inhibition of MMP-13 and S100A4.</p>

The study on HLA-A*02 allele frequency and its relationship with the susceptibility to lung cancer in Yunnan Xuanwei / 中国免疫学杂志

Objective:To study the frequency and distribution of HLA-A alleles and HLA-A*02 subtypes in the lung cancer patients in Yunnan Xuanwei area.This study aims at providing the significant genetic information for lung cancer research and treatment.Methods:Genomic DNA samples were collected from lung cancer patients and healthy donors residing in Yunnan Xuanwei area.HLA-A alleles and HlA-A*02 subtypes were typed by PCR-SSP assay.Case-control study was used to study the difference of frequency distribution between patients and normal controls.The polymorphisms of HLA-A*02 were also observed in cancer patients.Results:The positive rates of HLA-A*02 was significantly higher in cancer patients than in healthy controls (P=0.004,OR=2.432,95% CI=1.314-4.500),which were 68.75% and 47.50%,respectively.The gene frequency of HLA-A*02 was significantly higher in cancer patients than in healthy controls(P