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@#Objective To observe the effect of fastigial nucleus stimulation(FNS)on heart rate variability(HRV)of surgically induced myocardial infarction rats.Methods 100 Sprague-Dawley rats were randomly allocated in four groups,including sham-operation control group,rats with coronary arteries ligated but fastigial nucleus(FN)sham stimulated(AMI group),rats both coronary arteries ligated and FN stimulated(FNS group),and rats on which FN lesioned 5 d before,then coronary arteries ligated and FN stimulated(FNL group).HRV characteristics were determined 6 h,1 d,7 d and 21 d after the ligation,and mortality rates were observed after 21 d.Results FNS can improve the survival of myocardial infarction rats,and this may be due to the increased vagal tone and decreased sympathetic tone.Conclusion FNS may have cardio-protective effects on surgically induced myocardial infarction rats.
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@#The research on fastigial nucleus could be traced back to 19th century. However the great achievements on cerebrum overshadowed the importance of cerebellum and its nucleus. In recent 30 years, with the finding of brain protection effect of fastigial nucleus stimulation, much attention has been given to this field. Now its widely accepted that electrical stimulation of fastigial nucleus has brain protection effect and concrete mechanisms can be concluded as: improving the electrical instability around the infarct zone, reducing peri-infarction depolarizing wave and cortical spreading depression; suppressing microvessels inflammatory reaction; inhibiting cell apoptosis; reducing the neurons damage and facilitates neovascularization and so on. These new discoveries was reviewed and further expectation on this field was put forward by the authors.
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OBJECTIVE:To evaluate the effects of felodipine slow-released tablets on improving prethrombotic state of hypertensive patients.METHODS:Felodipine slow-released tablet(5mg)was given to36patients,q.d.for6weeks.Blood pressure,heart rate and hemostatic molecular markers including thromboxane B 2 (TXB 2 ),6-keto-prostglandin F 1? (6-k-PG_ F 1? ),tissue-type plasminogen activator(t-PA)and its inhibitor(PAI-1)as well as hemorheologic indexes were measured be_ fore and after felodipine administration.RESULTS:After the treatment,blood pressure was decreased significantly(P
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AIM: To construct a recombinant plasmid carrying enhanced green fluorescent protein and human vascular endothelial growth factor 121 gene and to detect its expression in rats mesenchymal stem cells (MSCs). METHODS: Human VEGF121 cDNA was amplified with polymerase chain reaction (PCR) from pCD/hVEGF121 and was inserted into the eukaryotic expression vector pEGFP-C1. The recombinant plasmid pEGFP/hVEGF121 was identified with PCR, double enzyme digestion and DNA sequencing. Then this recombinant plasmid was transfected into rat's MSCs with lipofectamine. The expression of EGFP and VEGF121 protein were detected with fluorescence microscope and immunocytochemical staining, respectively. RESULTS: The recombinant plasmid was confirmed with PCR, double enzyme digestion and DNA sequencing. The fluorescence microscope and immunocytochemical staining results showed that the EGFP and VEGF121 protein were expressed in MSCs 48h after transfection. CONCLUSIONS: The recombinant plasmid carrying enhanced green fluorescent protein and human vascular endothelial growth factor was successfully constructed and expressed positively in rat MSCs. It provides a good basis for further research on differentiation of MSC and VEGF gene therapy for ischemial cardiovascular disease.
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Objective:To investigate the effect of fastigial nucleus stimulation(FNS) on preventing oxidative damage from myocardium after acute myocardial infarction in rats.Methods:Wistar rats were randomly divided into acute myocardial infarction(AMI),FNS before AMI model was made(FNS+AMI),bilateral fastigial nucleus lesion(FNL) before AMI(FNL+AMI) groups.At 24 hours after coronary ligation,blood samples and left ventricular myocardium were obtained for measurment of the activity of lactic dehydrogenase(LDH) and creatine phosphokinase(CK) in serum,as well as myocardial infarct size.At the same time,the malondialdehyde(MDA) level,total anti-oxidative capability (TAOC) and superoxide dismutase(SOD) activity in myocardium were measured too.Results:LDH,CK activities and MDA level in FNS+AMI group were significantly lower than that in AMI group( P 0.05).Conclusion:FNS can attenuate the damage of myocardium from infarct heart and reduce myocardial infarct size.The mechanism of the protective effect coming from FNS may be related to its antioxidative role.