ABSTRACT
Objective To investigate the clinical features of type 1 autoimmune pancreatitis(AIP),and to deepen the understanding of this disease,reduce false positive rate,and enhance people′s awareness of this disease. Methods A retrospective analysis was performed for the clinical data of 13 patients with type 1 AIP who were admitted to The First Hospital of Jilin University from January 2012 to December 2016,including general status,clinical manifestations,laboratory serological examination,imaging findings,histopathological findings, treatment,and prognosis. Results Of all 13 patients,there were 9 male and 4 female patients with a mean age of 60.08 ± 9.47 years. Ma-jor clinical manifestations included jaundice(69.2%),abdominal pain(61.5%),and weight loss(61.5%). The most common organ in-volved was bile duct(46.2%),and 30.8% of the patients had sclerosing cholangitis. Of all patients,23.1% had diabetes. As for serolog-ical markers,92.30% patients had more than 2 times increase in IgG4,and 7.69% had 1-2 times increase in IgG4;53.85% patients had an increase in CA19-9;69.23% patients had an increase in total bilirubin;more than two thirds of the patients had an increase in amin-otransferases or gamma-glutamyl transpeptidase. As for imaging findings,53.8% patients had diffuse enlargement of the pancreas on CT, 46.2% had focal enlargement of the pancreas,and 46.2% patients had low-density cyst-like shadow in pancreatic lesions. Pathological examination showed fibrous connective tissue proliferation with infiltration of lymphocytes and plasma cells. All patients were given standard glucocorticoid therapy(initial dose of prednisone:30-40 mg/d)and the remission rate of glucocorticoid therapy was 100%. The follow-up time was 12 months,and one patient experienced multiple recurrences in the course of the disease. Conclusion Type 1 AIP is the local manifestation of IgG4-associated disease in the pancreas,which often occurs in middle-aged and elderly men,and most patients are com-plicated by extrapancreatic lesions. Glucocorticoid therapy is effective and most patients have good prognosis. Recurrence often occurs in the case of no standard or long-term glucocorticoid therapy.
ABSTRACT
Objective To study the inhibitory effect of hammerhead ribozyme targeting connective tissue growth factor(CTGF) on collagen I synthesis and cell cycle progression of human hepatic stellate cell line(LX-2) cells.Methods Hammerhead ribozyme cDNA targeting CTGF mRNA plus two self-cleaving sequences were inserted into pTriEx2 vector to construct a recombinant vector pTriCTGF-Rz.LX-2 cells were transfected with either pTriEx2 or pTriCTGF-Rz and further stimulated with or without TGF-1.There were five groups in the experiment:control group,pTriEx2 group,pTriCTGF-Rz group,pTriEx2 plus TGF-?1 group,and pTrCTGF-Rz plus TGF?1 group.Semi-quantitative RT-PCR was used to detect the levels of CTGF mRNA and collagen Ⅰ mRNA.ELISA and flow cytometry were used to detect the levels of collagen Ⅰ secretion and cell cycle.Results Transfection of pTriCTGF-Rz into LX-2 cells reduced the CTGF mRNA and collagen Ⅰ mRNA levels as well as collagen Ⅰ protein level compared with pTriEx2 group(P