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OBJECTIVE To study the current situation and problems of military blood emergency support and nosocomial infections,and find out countermeasures.METHODS The current situations and problems of military blood emergency support and nosocomial infections management system were analyzed,especially paying attentions to those parts including selecting blood donors,blood collection,storage,transport and distribution.RESULTS There were a lot of shortcomings existed in the blood infection factors control and management on blood emergency support;many measures should be taken to elovate the level of blood emergency,and reduce the risk of blood infection.CONCLUSIONS Improving and reforming the management system of blood emergency support in army,and maximatily reducing the risk of blood infection are important.
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0.05). Levels of TGF-?1 and PDGF in microparticle platelet frozen powder were higher than those in fresh platelet(P
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OBJECTIVE To study the sterilization by gamma radiation on the cytokine levels in manually manipulated platelet concentrates, and its practicability. METHODS Manually manipulated platelet concentrates were irradiated by gamma ray at 25 Gy. Detected the quantity of cytokine levels after irradiation on dd 0, 1, 3, and 5. Platelet counts and pH value were detected on the d0 and d5. RESULTS The contents of cytokines increased with storage time both in irradiation and control groups. But the contents of cytokines in the control group increased more significantly than in the irradiation one (P
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69 crabs were collected from Daxing, Gekui and Niukong townships of Lvchun county, Yunnan Province in 2006 and excysted metacercariae were only obtained from crabs of Niukong. The infection rate was 27.6% (8/29) with an average metacercaria number of 2.25 each crab. No encysted metacercariae were found. The excysted metacercariae were morphologically identified as Paragonimus proliferus.
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Objective To investigate the changes of vascular endothelial growth factor (VEGF),transforming growth factor-?1 (TGF-?1) and platelet derived growth factor (PDGF) in plasma and apheresis platelets with or without activation by shaking. Methods A total of 10 units of double therapeutic dose platelets were collected and each unit was divided into 3 groups to take samples of plasma (3ml/person,as control group),fresh apheresis platelets (5ml/person,as experimental group1) and activated apheresis platelets (platelets stored by shaking at 22 ℃ for 5 days,5ml/person,as experimental group2). The levels of VEGF,TGF-?1 and PDGF in the samples of the 3 groups were measured by ELISA. The expression of CD62p in 2 apheresis experimental groups was measured by flow cytometry. The differences between the two groups were compared. Results The levels of VEGF,TGF-?1 and PDGF,which were (410.95?95.07) pg/ml,(91.15?19.50) ng/ml and (12.60?2.06) ng/ml,respectively,in group 1 were markedly higher than those in the plasma control group,which were 149.09?28.11,37.38?10.73 and 3.28?0.79,respectively (P
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Objective To study the bacteriological effect of different dosages of gamma irradiation on Escherichia Coli (E. Coli) in red blood cells (RBC) products,and assess the appropriate dose of gamma irradiation. Methods Prepare RBC samples inoculated with(102-106)/ml of E. Coli. The contaminated RBCs were irradiated by 0 (as control),15,20,25,30 and 35Gy of gamma irradiation. E. Coli in the RBCs were tested on days 0,7, and 14 after irradiation. Results The quantities of E. Coli changed after irradiation. The RBCs inoculated with less than 103cfu/ml of E. Coli were completely sterilized by 25 Gy irradiation. Conclusion 25 Gy gamma irradiation can eradicate less than 103cfu/ml of E. Coli in the RBC products.