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0.05).Conclusion Cyclin B1 levels in liver cancer tissues were stronger than in adjacent cancer tissues and normal liver tissues.
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Objective To investigate the p33~ ING1b mRNA level and the relationship to clinical pathological data in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) method was employed to detect the mRNA level of p33~ ING1b in liver cancer tissues and adjacent tissues of cancer.Results p33~ ING1b mRNA was positive expression in 63.6% (35/55) cancer tissues and 70.9% (39/55) adjacent tissues of cancer.The expression level of p33~ ING1b mRNA in cancer tissues (0.410?0.175) was significantly lower than that in adjacent tissues of cancer (0.529?0.203). The level of p33~ ING1b was significantly associated with pathological grades, lymph node metastasis and envelope infiltration, but not associated with sex, age, tumor size, tumor thrombus or liver cirrhosis.Conclusion The decrease of p33~ ING1b may play an important role in the tumorigenesis and development of PHC, and may be an adjuvant parameter in evaluating tumor differentiation, invasion and prognosis.
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Objective To investigate the expression level and clinical significance of cyclinB1 in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of cyclinB1 in liver cancer tissues and adjacent tissues of cancer.Results CyclinB1 mRNA was positive expression in 90%(36/40)cancer tissues and 85%(34/40)adjacent tissues of cancer.The expression level of cyclinB1 in cancer tissues (0.531?0.015)was significantly higher than that in adjacent tissues of cancer(0.263?0.023).The level of cyclinB1 was significantly associated with pathological grades and lymph node metastasis, but not associated with sex、age、tumor size or tumor thrombus.Conclusion CyclinB1 may play an important role in the tumorigenesis of PHC and may be a potential adjuvant parameter in evaluating pathological grades and lymph node metastasis.
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Objective To analyse the necessity and feasibility of HCV RNA detection for donor screening.Methods Plasma from 50 donors was mixed into a minipool, the nucleic acid was extracted by NucliSens Extractor and amplified by NASBA, the amplification products were detected by ECL. A performance control and a positive control were coupled with the samples through storage, nucleic acid extraction, amplification and detection.Results Two specimens were found HCV RNA positive in the 10000 anti HCV negative samples.Conclusion HCV RNA screening of plasma minipools by NASBA may prevent hepatitis C virus infection.