ABSTRACT
Nosocomial infections are one of main causative agents of mortality among hospital patients. This study was done for the determination of efficacy of commercial disinfectants such as: formaldehyde, glutaraldehyde, microten, the alcohol 70 and savlonalcohol on isolated saprophytic fungi from hospital environments. This descriptive study was done on 33 isolated fungi from teaching hospitals of Tehran during 2009-10. The identified samples were randomly chosen. Stock fungal suspensions were supplied from each fungus with cells ranging 0.5-5_10[4] microg/cfu in 1ml with spectrophotometer at the wavelength of 530 nm. For evaluation of antifungal activity of commercial disinfectants formaldehyde, glutaraldehyde, microten, alcohol 70 and savlon-alcohol disinfectants, 0.25cc stock solutions were mixed with 3.75 cc disinfectants solutions and the new diluted samples held at 25c for 15, 30 and 60 min. The culture medium was checked for growth of fungi until 8 weeks. Following specific period isolated fungi were including Aspergillus spp with 39.4%, Penicillium spp with 36.4%, Fusarium spp with 12.1%, Rhizopus with 6.1%, Alternaria and Circinella with 3%. Formaldehyde 8% and glutaraldehyde 8% with activity against 63.6% and 39.3% were effective disinfectants at 15 min. Formaldehyde 8% with activity against 74.8% of fungi, was effective disinfectant at 30 min. Glutaraldehyde 8% and formaldehyde 8% with 100% prevention of growth were effective disinfectants at 60 min. According to this study formaldehyde 8% and glutaraldehyde 8% showed to have the highest antifungal activities. Synergetic fungicidal activity of comenercial disinfectants, dependent on time and concentration
ABSTRACT
The etiology of posttraumatic arthritis has not been studied well. Although it has long been assumed that articular incongruity leads to post traumatic arthritis, clinical and basic sciences evidence to support such claim are not sufficient. On the other hand, the relationship of instability due to anterior cruciate ligament deficiency and Arthrosis has not been clearly defined and the relative importance of instability and incongruity is unknown. ACL tearing were created in right knees of 9 rabbits [group A] and ACL tearing and displaced lateral condylar fracture with 1 mm step were created in another 9 rabbits [group B]. After 6 months, osteoarthritis changes were compared between control knee and injured knee of each rabbit and between the two groups. In both groups, osteoarthritis changes in injured knees were more comparing to the control knee and in whole osteoarthritis changes were significantly more in group B compared to group A. The unstable knee due to ACL deficiency goes toward osteoarthritis faster than normal knee. Incongruity in unstable joint increases intensity of osteoarthritis changes. It shows the importance of achieving anatomic reduction in intra articular fractures before ligament reconstruction