[Outcomes of heavy silicone oil tamponade in complicated retinal detachment surgery]

To evaluate the outcomes of internal tamponade using heavy silicone oil in complicated retinal detachment [RD] surgery. In this interventional case series, patients with complicated RD involving the inferior retina were enrolled. Inclusion criteria included RD secondary to proliferative vitreoretinopathy [PVR CP6 and/or CA6] involving the inferior retina, inferior or posterior tears, giant tears, penetrating trauma or RD combined with choroidal detachment. Heavy silicone [Oxan HD, Bausch and Lomb, USA] was injected at the end of surgery after peeling of retinal membranes or retinotomy. Follow-up examinations were scheduled one day, one week, one month and four months after surgery. Additional visits were made depending on the condition of the eye. Seventeen eyes of 17 patients with mean age of 45.4 +/- 12.2 [range 19-71] years underwent vitrectomy and heavy silicone oil tamponade. Mean follow-up was 11.4 +/- 3.5 [range 4-16] months. There was total RD in 10 cases, subtotal RD involving the inferior retina in 6 patients and RD associated with choroidal detachment in one. Best-corrected visual acuity [BCVA] before surgery was LP or HM in 13 eyes and counting fingers at 0.5 to 3 m in 4 eyes. During the follow-up period, the retina remained attached in 12 cases [70/5%] but 5 eyes needed further vitrectomy for reattachment. BCVA improved from 1.95 +/- 0.09 LogMAR preoperatively to 1.5 +/- 0.35 four months after the operation [P=0.045]. After 4 months' follow-up BCVA was HM in 4 eyes and 0.5 m to 20/200 in 13 eyes. Transient IOP rise occurred in one eye and silicone oil emulsification was seen in another eye. Heavy silicone oil tamponade had favorable results in terms of anatomical attachment of the retina and visual acuity

[Prevalence of lipodystrophy associated with human recombinant insulin]

Lipodystrophy is potentially a clinical adverse effect, associated with insulin therapy and is believed that usage of human recombinant insulin's is associated with decreasing prevalence of Lipodystrophy. The objective of this study was to determine the prevalence of insulin induced Lipodystrophy, among diabetic out-patients referred to Imam Khomeini Hospital, in Sari during 2007. In this cross sectional descriptive study, 220 diabetic patients referred to the Diabetes Center at Imam Khomeini Hospital, in Sari, who under treatment by insulin at least three months prior to referral was evaluated. First, the demographic and clinical characteristics of the patients were recorded in a questionnaire; then all patients were examined clinically to evaluate lipodystrophy. In all subjects, glycated hemoglobin [HbA1C] was measured to assess the range of blood glucose level control. Recorded data were analyzed by statistical methods, such as descriptive T-test and X[2].Of 220 diabetic patients studied, thirty-five [15.9%] showed clinical evidences of insulin induced Lipodystrophy; 32 out of 35 cases of Lipodystrophic patients [14.5%] had Lipohypertrophy, while 3 cases [1.4%] had Lipoatrophy. The factors included Age, Sex, Education, BMI [Body mass index], type of Diabetes, The duration of insulin consumption and injection site had statistically significant effects on development of insulin induced Lipodystrophy [P<0.05]. The results of this study demonstrated that despite using human recombinant insulin's, the prevalence of insulin induced lipodystrophy, especially Lipohypertrophy, has remained high up to present. Therefore, regular examination of patients for this side effect is necessary, especially in subjects without good control of blood glucose level

[Role of anxiety in idiopathic acute anterior uveitis]

To evaluate the role of anxiety in idiopathic acute anterior uveitis. In this case-control study, three groups including 30 patients with uveitis, 30 patients with conjunctivitis, and 30 healthy control were enrolled for estimating the anxiety level by employing Zung anxiety assessment and a structured clinical interview. T test and Chi square test were used for statistical analysis. Mean anxiety level was 37.5, 33.5, and 33.5 in the anterior uveitis, conjunctivitis, and control groups, respectively. [P<0.001] Severe anxiety was present in 23%, 7% and 17% of the uveitis, conjunctivitis and the control groups, respectively. The individuals were male in 60%, 53%, and 57% and were married in 77%, 70%, and 83% of the uveitis, conjunctivitis and control groups, respectively. Mean age uveitis, conjunctivitis, and control groups was 42.6 yr, 33.6 yr, and 31.1 yr, respectively. [P<0.01, F=3.09] The present findings have verified that anxiety may be effective in idiopathic acute anterior uveitis

Detection and cloning of desulfurization operon sox [dsz A and B] in rhodococcus FMF native bacterium

Native Rhodococcus FMF bacterium was selected among several isolated Iranian bacterium. Primary studies have shown that the bacterium use dibenzothiophene as a sulfur source. Since desulfurization is taken by a biochemical pathway, the present project was designed to detect, clone and sequence of desulfurization genes of the bacterium. Desulfurization operon was isolated from Rhodococcus erythropolis IGTS8 and was used as a probe for detection of desulfurization 4S pathway genes by southern blotting technique. Specific primers were designed and the dsz A and B genes were amplified by polymerase chain reaction [PCR]. PCR products were recovered from agarose gel and were cloned into the PTZ57R vector. In this study, recombinant PTZAB57R vector was constructed by the insertion of dsz A and B genes into the PTZ57R vector. The clones were confirmed by restriction digestion analysis. The PTZAB57R was extracted by large-scale method and was sequenced [MWG DNA Biotech]. Comparison of sequences of dsz A and B genes from native Rhodococcus FMF bacterium with Rhodococcus sp. IGTS8 bacterium were shown complete identity between them, which show that desulfurization pathway is conserved in this bacterium