Xenobiotic metabolising enzymes in DLA induced Balb/c mice and on treatment with protein fractions of Cynodon dactylon and Aegle marmelos

The investigation on xenobiotic metabolising enzymes have been carried out in mice liver. In the present study, in vivo studies were carried out using BaIb/c mice. DLA cells were transplanted intraperitoneally and ED[50] dose of protein fractions of Cynodon dactylon and Aegle marmelos were injected intraperitoneally once a day during the course of the study. The levels of xenobiotic metabolizing enzymes phase I [Cytochrome b[5] and P450] and phase II [Glutathione S-Transferase] were found to be increased and decreased in DLA induced mice. Administration of protein fractions significantly reversed the level of enzymes both in the presence and absence of DLA cells. These results indicated the protective role of protein fractions

In vitro cytotoxic and antimutagenic effects of protein fractions of cynodon dactylon, aegle marmelos, ocimum sanctum and coleus aromaticususing DLA cell lines

With increasing knowledge of the stages of initiation and development in the natural history of neoplastic diseases and the multiple observations that such stages are likely to be ubiquitous in the natural history of all histogenetic types of neoplasm, it is becoming increasingly important to distinguish chemical agents that act selectively during one or the other of these stages. Several compounds, occurring in food, were tested for antimutagenic effect towards cigarette-smoke condensate [CSC] and Benz [a]pyrene[BaP]. Antimutagenicity was determined in the Salmonella/ microsome test, with tester strain TA 98, in the presence of rat-liver homogenate. The similarity in results for cigarette-smoke condensate and for BaP indicates that a general mechanism may be involved in the inhibition of CSC and BaP- induced mutagenicity