ABSTRACT
Background: Radiotherapy is an important factor which results in increase of anastomosis leakage. Diverting loop ileostomy has been usually performed after colorectal anastomosis with history of neo-adjuvant radiotherapy to decrease the chance of leakage. Considering this effect, we assessed the feasibility and outcome of human amniotic membrane in rectal anastomosis in dogs previously treated by high-dose radiotherapy
Materials and Methods: Twelve cross-breed male dogs with the age of 6-8 months and weighting 10-15 kg were randomly divided into four groups. Groups 1 and 2 received radiotherapy and 4 weeks later, single layer end to end anastomosis was performed for all the dogs; also, diverting loop ileostomy was placed in groups 1 and 3. Four weeks later, the anastomosis site was resected and sent for pathologic wound healing scoring. Data were analyzed by SPSS 16 software using Mann- Whitney test
Results: Two dogs [of group 1 and 2] died of peritonitis due to anastomosis leakage during the 2[nd] post operation week. There was no statistically significant difference in wound healing between the case and control groups [P-Value: 0.01]
Conclusion: HAM had a protective role in colorectal anastomosis after neo-adjuvant radiation in cases without loop diverting ileostomy. It can be concluded that HAM placement is a feasible technique instead of diverting loop ileostomy in cases with neo-adjuvant radiotherapy and also in the benign colorectal diseases with high risk anastomoses