ABSTRACT
Introduction: Leptospirosis is a common Zoonosis, which is more prevalent in tropical and temperate regions. Rodents, wild and domestic animals are reservoir of Leptospirosis. Usually the infected animals are carriers for the rest of their lives and the bacteri is secreted from their urine. The secreted bacteria can enter a host [animal or human] and continue the circle of disease
Objective: Diagnosis of leptospirsis according to clinical symptoms is difficult due to lack of pathogenic sign[s] and the similarities of its clinical features to some common febrile diseases, so laboratory is important in diagnosis. Leptospira is fastidious and its isolation from other clinical specimens is difficult, time consuming and usually unsuccessful, so MAT is the gold standard for diagnosis and serotyping of leptospira and is usual in all reference laboratories
Materials and Methods: We performed this study in 1383 by taking blood sample from patients hospitalized in Emam Khomeini Hospital in Some- e-sara, Razi and 22 Aban Lahijan and had clinical symptoms and were suspected of leptospirosis. Sampling was done in spring and summer, which are prevalent seasons of leptospirosis in Gailan prorince. We stored all serums in- 200[degree]c until examination, and in summer of 1383 serum samples were screened by a Quantitative Elisa method to detect positive samples for doing MAT, and performed MAT to determine causative serogroups
Results: 282 serum sample were all tested by quantitative Elisa and 130 cases had IgM titer equal or greater than 1:60 which were considered for MAT test. Seventy serum samples had titer
Conclusion: Analyzing the results of MAT was hard and complex due to alternate reactions happening between different serogroups specially in clinical samples related to acute disease. According to CDC, a serum titer of >/= 200 and if it correlates with clinical symptoms can present probable diagnosis. In this study, considering the samples were only taken from patients with clinical symptoms and suspected of leptospirosis and then after screening by Elisa, only positive samples with IgM titer equal or greater than 1:16 were assessed by MAT keeping in mind three criteria [Correlating clinical symptoms Elisa and MAT results] there is a high efficacy of diagnosis and determines servoirs and serogroups are prevalent in this area
ABSTRACT
Introduction: Leptospirosis is most widespread zoonosis in the world and is prevalent in tropical and temperate regions. Diagnosis of leptospirosis by clinical symptoms is difficult due to lack of specified pathogenic signs and similarity of its clinical symptoms to some bacterial and viral febrile disease, so laboratory plays an important role and ELISA is a common serological method. Animal leptospirosis is common in regions of Iran where traditional and unmechanized husbandry are used. But human leptospirosis is only prevalent in Guilan and Mazandaran and is endemic in Guilan. Factors such as climate conditions, wild animals, rice plantations, environmental water and finally keeping pets by traditional ways all contribute to this disease in Guilan
Objective: This study was done to determine the epidemiological characteristics of leptospirosis in Guilan
Materials and Methods: The sample of this study consisted of all suspected persons who referred to general hospitals of Guilan province. Blood samples were taken from these patients. All blood samples were examined by a commercial ELISA kit to detect positive cases and determine epidemiological features of this disease in this province
Results: Findings show that most of positive cases belong to males [62.37%] than females [27.23%], and in farmers [86%] and in warm months [90% from first of Khordad to end of Sharivar], and its frequency in middle age [20 - 50 year olds] was almost 65%
Conclusion: The geographical spread of the disease was more in big cities of Guilan province where more villages had and were crowded as well. This study showed that most cases of disease was detected in people who are somehow involved in farming