ABSTRACT
Background: the expression alterations of specific miRNAs were analysed in response to gamma-irradiation in two breast cancer cell lines MCF-7 and MDA-MB- 231 with low and high BCSCs content to design probable strategies for sensitizing breast cancer stem-like cells MDA-MB-231 to radiation
Materials and Methods: the expression levels of hsa-miR-34a, hsa-let-7i and hsa-miR-21 were assessed by QRT-PCR in MCF-7 and MDA-MB-231 breast cancer cells at three time points after gamma irradiation using three different doses
Results: the results showed that the over-expression of hsa-miR-34a in MCF-7 cells was much more significant than MDA-MB-231 cells. Furthermore, there was a considerable overexpression of hsa-miR-21 in MDA-MB-231 cells following exposure to ionizing radiation [IR]. The hsa-let-7i expression changes were different, depending on radiation dose, time post irradiation and cell type
Conclusion: according to the results, we may be able to sensitize stem-like MDA-MB-231 breast cancer cells to radiation by increasing miR-34a and decreasing miR-21 expression levels simultaneously
ABSTRACT
Celiac disease [CD] may be misdiagnosed as Irritable Bowel Syndrome [IBS] resulting in long delays in diagnosing CD. There are contradictory reports on the association of CD with IBS. Appropriateness of screening all patients with IBS for CD and how to screen them are still under question. In a cross-sectional study, 328 IBS patients [Rome II] referred to the Poursina Hakim Gastroenterology Clinic were investigated for CD. Total serum anti-tissue transglutaminase IgA [anti-tTG IgA] concentration was measured in all patients. In IgA deficient cases, antigliadin antibody [AGA] IgG concentration was also measured. Moreover, in patients who underwent upper endoscopy [as their necessary workup] duodenal biopsies were taken. Fifty-eight patients were excluded. The remaining patients were 166 [61.5%] women and 104 [38.5%] men with the mean age of 35.3 years [SD = 11.8]. No one had positive serological test of IgA anti tTG antibody. Five patients were IgA deficient; none of them had positive IgG AGA. Duodenal biopsies were taken in 60 patients and pathologic evaluation showed 53Marsh 0, three Marsh I, three Marsh II, and one Marsh IIIa. Only the patient with Marsh IIIa adhered to gluten-free diet [GFD] which led to decrease in severity of symptoms. In patients who did not adhere to GFD, no one had positive serological test after 12 months of follow-up. Prevalence of CD in patients with IBS referred to outpatient gastroenterology clinic might be significant but serum anti-tTG IgA antibody is not helpful in detecting CD in these patients. Further studies are needed to clarify this issue