ABSTRACT
Dot-ELISA assay is a solid phase diagnostic method for detection of antigen or antibody that is used widely for diagnosis of protozoan and metazoan diseases of human and animals. To evaluate this method in diagnosis of human toxoplasmosis, the test was standardized, using golden positive and negative serum samples. Then 215 human serum samples were evaluated for IgG and IgM against Toxoplasma gondii by Indirect Immuno-Fluorescent Antibody [IFA] and Dot-ELISA. Using statistic program, Epi info 6.0 showed these testes have a good agreement in diagnosis of toxoplasmosis with Kappa = 0.8607 for lgG and Kappa = 0.8865 for IgM antibodies [P<0.05]. This study and the works carried out by the other scientists indicate that the Dot-ELISA test is rapid, simple, cost effective, does not need expensive equipment and has a good sensitivity and specificity. But, as the results are expressed qualitatively, therefore, it is not possible to use for antibody titer determination. Yet, it is quite useful for screening test especially in the field and where there are no well-equipped laboratories