ABSTRACT
Nasopharyngeal samples were collected from 38 children suffering from acute otitis media by tympanocentesis, the samples were cultured for bacterial and viral isolation, neat samples were used for the detection of respiratory SV adenovirus antigen by ELISA test beside the culture. Bacterial culture was positive in 28 out of 38 cases, were isolated in percentages of Depthehroid 28.75%, Proteus 21.42%, Microcous 17.86%, then Streptopyogen, Pneenococcus, Pseudomonas, and Staph., each of them was 7.14%. Viral isolates in tissue culture were positive in 12 out of 35 cases, were isolated by value of 25% RSV, 16.7% adenovirus, 16.7% entervois and 41.66% undiagnosis. The ELISA test from the neat serum were positive in 8 cases for adenovirus and 5 RSV cases. No relationships between bacterial and viral infection in AOM of children