ABSTRACT
The subtilisin complexes with dexamethasone and Alpha-2 macroglobulin were synthesized and were found to be immunoreactive to the specific antidexamethasone antibodies. These complexes also retain proteolytic activity when low molecular weight substrate was used by Homogeneous colorimetric method. The doubly labelled substrate was also used to study the enzyme activity in complex form. The Dex-subtilisin showed the proteolytic activity whereas Dex-subtilisin A2M did not show the proteolytic activity due to no access of small molecular weight substrate towards the large molecular weight protein in a co-complex form
ABSTRACT
The different assay methods for the determination of chloroquine phosphate have been reviewed. The quantitative determination of Chloroquine phosphate from commercial pharmaceutical preparations have been analysed by acid-base, non-aqueous and spectrophotometric methods and their results have been compared. The spectrophotometric method has been found to be a better method for the determination of percentage purity of Chloroquine phosphate in pharmaceutical preparations