ABSTRACT
Fifteen Swiss albino male rats were used to study the effect of hyperthermia on the mucosa of the small intestine. Five rats were used as a control group. The other 10 rats were exposed to humid air at 43C for 2 hours/day. Five animals were sacrificed after 24 hours and the other five were sacrificed after one week. After 24 hours, the microvilli were low and conical in shape reflecting a primary effect on the cytoskeleton of the cells. One week post-hyperthermia, abnormal stacking of enterocytes, swollen mitochondria, dilated rER, thickening of the terminal web and damages in the endothelial cells of the blood capillaries were seen
Subject(s)
Animals, Laboratory , Hyperthermia, InducedABSTRACT
Ketoconazole is an oral antifungal drug used to treat systemic and superficial fungal infection. It has several side effects, the blockage of testosterone biosynthesis is one of the most important. The ultrastructural changes of the seminiferous tubules of testies of rabbits are investigated. Ten adult male normal rabbits were given the drug orally in a dose of 3 mg/kg body weight every day for one month. Five control animals were also used. Testicular specimens were taken, fixed and processed for ultrastructural study. Variable degrees of degenerative changes were seen in the spermatogenic cells, varied from vacuolation of the cytoplasm and degenerated mitochondria to complete destruction of the nucleus
Subject(s)
Animals, Laboratory , Seminiferous TubulesABSTRACT
Twenty-five albino rats were included in this study, five were used as a control group and 20 animals were exposed to the same concentration of chlorine vapors used in industries and domestic purposes. Ten animals were sacrificed after 2 months of chlorine exposure, the other 10 after 3 months and the 5 control animals were killed simultaneously. The eyeballs were dissected and processed for a light microscopical study using the routine H and E, trichrome, PAS and reticulin stains. Prolonged exposure to chlorine vapors produced structural changes in the cornea. Mitotic figures appeared in the basal cells of the corneal epithelium, the Bowman's membrane was thin, irregular in some parts and more filamentous material was deposited in the epithelial cells. Edematous areas between the stromal lamellae were seen. The endothelial layer was markedly thickened, with intercellular edema, hydropic degeneration and fibrillation of the Descement's membrane
Subject(s)
Animals, Laboratory , Cornea/drug effectsABSTRACT
Among the many factors responsible for pulmonary damage after major surgery and trauma are free fatty acids. This study was done to find out the acute histological and histochemical changes in the lung of experimentally induced fat embolism syndrome. Oleic acid was injected directly into the right atrium of 10 dogs [study group]. Two dogs were used as control. All dogs were anesthetized, mechanically ventilated and subjected to midline sternotomy. Histological and histochemical study of lung biopsy two hours after embolization revealed narrowed and atelectatic alveoli, plasma and hemorrhagic exudation, hyaline membranes, widened interstitium with massive edema, mononuclear cellular infiltrate, marked congestion of the capillaries and increase in the protein content. There was a definite increase in the activity of both acid and alkaline phosphatase enzymes. This reflected the intensity of the exudative inflammatory reaction and increased vascular permeability, respectively. These findings provided an explanation of the hypoxemia encountered in the clinical settings of respiratory insufficiency following major trauma and surgery and emphasize the role of positive end expiratory pressure to open up the collapsed alveoli