Factors affecting viability of mycoplasma in culture media

A microculture technique was used to evaluate the effect of different types of media, buffers, sera and dextrose concentrations on the viability of Mycoplasma gallisepticum [MG] S6, F and L strains and Mycoplasma synoviae WVU 1853 and S27 strains. The initial pH of the culture media was adjusted to 8.5. B-medium of Hayflick without dextrose prolonged MG viability 5-6 days at 37C and MS 4-5 days at 37C but plate count was low. Five types of buffers used did not increase viability. Tris and veronal buffers inhibited MS growth. Dextrose although increased plate count [yields], it decreased viability period because its fermentation dropped the pH of culture medium which lysed mycoplasmas. No growth of MG below pH 5.2 and no growth of MS below 6.2. PPLO serum fraction prolonged viability to 7 days for MG and 6 days for MS but plate growth was very low

use of soft laser for the treatment of lymphoepithelial lesion of the parotid gland, [Mikulicz's disease]

In the present-study, a case of lymphoepithelial lesion of the parotid gland was reported in which there was a symptomatic bilateral parotid gland enlargement, the histopathological features shows epimyoepithelial cell islands and marked lymphocytic infiltration. There was severe xerostomia with difficulty in deglutition. Using the recent diagnostic aids a trial was made to establish a definite criteria for this disease. Soft laser was used for the treatment of this case which resulted in marked decrease of the swelling and improvement of xerostomia to certain extent identified by easier swallowing of food and decreaseing of the daily water intake. Post treatment true cut needle biobsy for the left parotid showed disappearance of the tumor cells with marked regeneration of the glandular tissues, and the diagnosis was nonspecific granulation tissues