Immunohistochemical localization of human papilloma virus antigen in oral squamous cell papilloma, epithelial dysplasia and squamous cell carcinoma

Thirty-eight cases of oral lesions represented 5 cases of epithelial dysplasia, 19 squamous cell papilloma and 14 squamous cell carcinoma. Nuclear staining reaction for human papilloma virus [HPV] was observed in 10 cases of squamous cell papilloma, 2 epithelial dysplasia and 8 squamous cell carcinoma. A correlation between koilocytosis and HPV was observed. Cytoplasmic staining for HPV could be taken as an indicator for the diagnosis of squamous cell papilloma, as it was detected in 5 cases of it, and was not observed in dysplasia or carcinoma. HPV was found to be significantly prevalent in squamous cell papilloma than both dysplasia and carcinoma, which may point out to an important role of HPV in squamous cell papilloma than dysplasia or carcinoma

Intracerebroventricular [ICV] administration of insulin lowers plasma glucose level in the rat

The effect of the central administration of insulin on plasma glucose and immunoreactive insulin [IRI] in normal and diabetic rats has been determined. Insulin [400 muU/kg/5 mul] was injected into the third cerebral ventricle stereotaxically. Blood samples were taken after 10, 30, and 60 minutes from insulin injections by heart puncture. In normal rats, the central administration of insulin significantly lowered plasma glucose level. A significant increase in plasma IRI level was also evident. Intracerebroventricular insulin-induced hypoglycemia was completely blocked by the prior administration of atropine [100 ug/kg i.p.]. In streptozocin [STZ] diabetic rats, intracerebroventricular injected insulin induced a significant fall in plasma glucose, but plasma IRI was not significantly altered. The role of this central action of insulin on plasma glucose regulation was discussed with special reference to other already known mechanisms