Optimization of cellulase production by a thermotolerant aspergillus terreus R4 from filter mud

Seven strains of thermotolerant Aspergillus species were screened for cellulolytic activities. Aspergillus terreus R-4 was selected as the most potent cellulase producer on filter mud [FM], as a new substrate, produced as a by-product in cane sugar industry. CMCase, FPase and cellobiase production were optimal when 10% inoculum was used. The optimum fermentation conditions consist of medium composed of [g11]: FM, 25, NH[4]CI, 2, HKH[2]PO[4]; MgSO[4-], 7H[2]O, 0.5; NaCI, 0.5 at pH 7.0 and incubation period for 7 days under shaking conditions