ABSTRACT
Evaluation of autogenous fibrinogen extracted from horse blood in fibrin glue and it's effects on horse's fibroblast and keratinocyte cells were preformed. Fibrinogen has been obtained by glycine precipitation technique from horse's blood. Fibroblast cells were separated by explant culture method from neck skin and keratinocytes were separated by enzyme digestion from lib samples. Autologous fibrinogen, horse`s fibroblast and keratinocyte cells separately mixed with bovine thrombin, calcium gluconate and tranexamic acid. This mixture was uniformly spread on cell culture plate and evaluated for six days. After one day of culture the thickness of fibrin glue were observed by invert microscope, fibroblast cells in elongated shape were observed in the fibrin glue in all thickness and keratinocyte cells were flattened and adherent on surface of plate. Both of them proliferated after 3 days. Fibrinogen that was obtained from horse blood by glycin precipitation technique can support horse fibroblasts as a scaffold and support horse keratinocyte proliferation in vitro
Subject(s)
Animals , Fibroblasts , Keratinocytes , HorsesABSTRACT
The study was conducted to study the effect of hormonal treatment on Barrenness ewes reproductive performance. Fertility [number of ewes lambed per ewes joined], Twinning percentage [number of twinning lambing per ewes lambed],Lambing percentage [number of lambs porn per ewes joined], mortality [number of lambs died ber lambs born] and weaning percentage [lambs weaned per ewes joined] for 25 Barrenness ewes were treated with Veteglan [Cloprostenol] and HCG .Reproductive performance were affected significantly [P<0.05] by hormonal treatment Treated ewes were surpassed control group in there fertility [73.0% compared with 40,0%], twinning percentage [9.1% compared with 0.0%], lambing percentage [80.0% compared with 40%], mortality [25.0% compared with 50.0%] and weaning percentage [60.0% compared with 20.0%] It was concluded that hormonal treatment increased fertility and weaning percentage in barrenness ewes by 33.0% and 40.0% res