ABSTRACT
Cutaneous leishmaniasis caused by L. major is an important public health problem in endemic areas. The aim of this study was to explore the therapeutic effect of microwave and or infrared radiation in the treatment of lesion induced in BALB/c mice by L. major inoculation. The footpad lesion was induced in BALB/c mice by inoculation of L. major promastigotes subcutaneously. The lesion was treated with 600 watts power, 2.450 GHz frequency and/or infrared device with 150 watts and a wave length of 890 nanometres. The size of the lesion was recorded by footpad swelling measurement ever 10 days. The lesion growth was significantly hampered in treated mice compared with the untreated control group [P<0.05]. Infrared radiation was more effective than microwave in inhibiting ulcer enlargement. Infrared radiation and microwave significantly hampered L. major lesion growth in BALB/c mice. This therapeutic effect was more in infrared radiation treated mice than microwave treated mice
ABSTRACT
Determination of the division histoty of T cells in vitro is helpful in the study of effector mechanisms against infections. Technique described here uses the intracellular fluorescent label carboxyfluorescein diacetate succinimidyl ester [CFSE] to monitor the proliferation. In a cross sectional study, blood samples were collected from 7 volunteers with history of cutaneous leishmaniasis [CL] and one healthy control from endemic areas in Isfahan province who referred to the Center for Research and Training in Skin Diseases and Leprosy [CRTSDL], then CD4[+]/CD8[+] lymphocytes and CD14[+] monocytes were isolated from peripheral blood mononuclear cells [PBMC] using mAbs and magnetic nanoparticles. CFSE labeled CD4[+] or CD8[+] lymphocytes cultured with autologous monocytes in the presence of PHA, SLA, live Leishmania major or as control without stimulation. Cells were harvested after 7 days and were analyzed using flow cytometry. Five consecutive divisions were monitored separately. Stimulation of CD4[+] or CD8[+] lymphocytes from CL subjects with SLA showed a significant difference in proliferation comparing with unstimulated cells [P< 0.05]. The significant difference in the percentages of CD4[+] cells stimulated with SLA was revealed at different divisions for each subject. In CD8[+] lymphocyte, significant stronger stimulation of SLA was evident later in the proliferation process. The mean number of divisions in both CD4[+]/CD8[+] lymphocytes stimulated with SLA was significantly greater than when stimulated with live L. major [P=0.007 / P=0.012, respectively]. The percentage of divided cells might be calculated separately in each division. The cells remained active following CFSE staining and there is possibility of functional analysis simultaneously