ABSTRACT
In recent years, the incidence of opportunistic fungi has shown a marked increase. Infection caused by common pathogenic fungi is a significant health problem in immune compromised hosts. The present study evaluated antifungal activity of Titanum dioxide nanoparticles and Ethylene diamine Tetra-acetic Acid against Candida albicans as self-cleaning agent by standard micro dilution test. The present study was conducted at the Medical University of Tarbiyat Modares in 2009. TiO2 nanoparticles were obtained through the hydrolysis of TiCl4 [Titanium tetrachloride]. Size and type of these nanoparticles were characterized by scanning electron microscopy [SEM] and X-Ray-Diffraction [XRD]. Afterwards, the Minimum Inhibitory Concentration [MIC] and Minimal Fungicide Concentration [MFC] test for TiO2 and EDTA were performed. Concentration of synthesized TiO2 was 7.03 mg/ml and 5.63 x 10[20] particles/ml. evaluation of morphology and diameter of the TiO2 nanoparticles with SEM showed that nanoparticles were spherical with diameter between 40-65 nm. MIC50 of 2.2, 1.24 and 0.125 micro g/ml respectively. MIC90 and MFC of TiO2, EDTA and fluconazole were 3.51, 2.48, 0.5 micro g/ml and 4.06, 3.1, 1 micro g/ml respectively. In the present study, using of synthesized TiO2 nanoparticles with chemical method showed a suitable activity against Candida in comparison with Fluconzaole. Thus it might represent a good candidates in elimination of Candida in medical from medical devices