ABSTRACT
Feline hemotropic mycoplasmas are parasites of erythrocytes and include three species, Mycoplasma haemofelis, Candidatus mycoplasma haemominutum and Candidatus mycoplasma turicensis. Diagnosis of the infection with these microorganisms can be carried out using conventional assays such as blood cytology. However, these assays have a low accuracy and a high rate of false-positive results due to the poor techniques and procedures and high occurrence of artifacts. Therefore, molecular techniques such as polymerase chain reaction [PCR] are better methods for the diagnosis of infections by these bacteria. The purpose of the present study was to evaluate Feline hemotropic mycoplasma prevalence and phylogenetic analysis in Tehran. Sixty cat blood samples were collected from veterinary clinics in Tehran from 2011 to 2012. Giemsa stained blood smears have been examined by the light microscopes and the positive samples were used for DNA extraction and PCR. Positive PCR samples were sequenced for the differentiation of bacterial species and phylogenetic analysis. Thirty-two samples were positive in direct examination from which two samples were identified as M. haemofelis by the PCR. No positive samples of C. M. haemominutum or C. M. turicensis were found in PCR. Phylogenetic analysis of the isolates showed that these isolates were more similar to the isolates from China and Thailand compared to those from other countries. This study is the first report of phylogenetic analysis of hemotropic mycoplasmas in Iran. Based on the high sequence similarity between Iran, China and Thailand isolates, it can be concluded that these bacteria possibly had the same origin
Subject(s)
Animals , Cat Diseases , Blood , DNA , Polymerase Chain ReactionABSTRACT
Honey has been generally used in traditional medicine for healing different kind of wounds. In this research complex, the clinical and histopathological effects of West Azarbayjan non-boiled honey in healing process of surgical wounds [cuts] were evaluated. This study was performed to evaluate the effect of honey on healing time and duration of wound closing in dog. 30 dogs, each weight was almost 25kg, were divided into 2 clinical [n=10] and histopathological [n=20] groups. In each group, after surgical preparation and anesthesia surgical wounds were produced by a similar pattern and size [rectangle; 25×50 mm[2]] in the thoracolumbar region. Wounds on the left side arbitrary taken as treatment group and on the right side as control group. Postoperative treatment in the honey group was included daily wound irrigation with normal saline and then topical application of 20g honey. In the control group only irrigation with normal saline was performed. Clinical and histopathological parameters were evaluated in each group 7, 14, 21 and 28 days after operation. Honey caused in acceleration an healing and surgical wound closure and in decreasing the level of infection and secretion in the experiment group in compare to control group which was more significant at days 14 and 28 [p<0.05]. Decreasing the area of wound during experiment in honey treated group was 11.1% more than control group. From histopathological aspect, especially from the second week on, less neutrophilic chemotaxis was found in treatment group than in the control group and in treatment group the compact connective tissue was developed more rapidly. West Azarbayjan non-boiled honey is potentially effective in surgical wound healing
Subject(s)
Animals , Wound Healing , DogsABSTRACT
Rabies is acutely fatal encephalitis caused by a neurotropic virus. This virus belongs to the family of Rhabdoviridae and genus of Lyssavirus. The virus is almost always transmitted to human through infected mammalian saliva. Rabies is inoculated to a wound by an infected animal bite. Since infection is established in the CNS, the outcome is almost always fatal. According to the outstanding role of vaccination against rabies in animals, as well as post-exposure treatment regimen in human, production of cell-cultured rabies vaccine is the most common interest of researchers. Two BHK-rabies vaccines, one made in the Pasteur Institute of Iran and two in Schering-Plough Animal Health of Madrid, Spain have been tested on 12 dogs divided into two groups. Sera were taken monthly over 18 months. To evaluate the titer of the rabies-neutralizing antibody, these sera were analyzed by the rapid fluorescence focus inhibition test [RFFIT] in the end of each month. Both study groups showed a sufficient immunogenic response without any significant difference at least one year after vaccination. With respect to the effective immunization of rabies vaccines, their annual injections would be sufficient. According to the results, at the end of the study [18th month] serum titer in only one dog [No.7] was less than protective level. Two animals in group one [No.2 and No. 5] also had serum titers less than protective level. Mean of post vaccination antibody titer were not different in either groups [P=0.35]
ABSTRACT
In present study the zoonotic role of cat in Bartonella henselae transmission have determined. It has done on 100 cats in 2 groups: indoor and outdoor and in 2 age's subgroups. Bartonella henselae was not isolated from blood culture of cats. 23 cats from 100 cats [23%] had antibodies against B. henselae. In this study there were no significant differences statistically in seroprevalence between cats and their owners [p<0.381]. Seroprevalence of cat owners was 18% and in control group [persons who own no cat] was 5%. There were significant differences [p<0.004] between cat owners and control group. Only 6 cats of 50 cats under 6 months old had antibodies to bartonella henselae, and in the other group 17 cats were seropositive and there were significant differences between these two groups [p<0.009] that showed seroprevalence in cats more than 6 months old is higher than the cats under 6 months old. 2 indoor cats from 50 indoor cats and 21 outdoor cats from 50 outdoor cats were seropositive and comparing of these two groups showed significant differences [p<0.0005], which confirmed indoor cats are less frequently infected than outdoor or stray cats