ABSTRACT
The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene [ESR1] in Barbari bucks [fertile and non-fertile] identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction [RT-PCR]. The expression pattern of ESR1 gene was analyzed by RT-PCR [Roche LC-480]. Relative quantification by RT-PCR indicated that the ESR1 gene expression showed more fold in fertile bucks as compared to non-fertile
ABSTRACT
This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR[2]aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes [COC's] were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oocytes [n=226] were exposed to 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP for 4 h in mCR[2]aa medium. Group 2 in vitro matured oocytes [n=294] were exposed to 7% ethanol for 5 min followed by treatment with 10 micro g/ml CHX for 4 h in mCR[2]aa medium. Group 3 in vitro matured oocytes [n=325] were exposed to 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP and 10 micro g/ml CHX for 4 h in mCR[2]aa medium. Group 4 in vitro matured oocytes [n=108] were cultured for 4 h without any chemical treatment in mCR[2]aa medium [control]. The cleavage rate in groups 1, 2, 3 and 4 was 54.42%, 44.55%, 51.69% and 0.00%, respectively. The percentage of morula and blastocyst production in group 1, group 2 and group 3 was 26.01%, 29.77% and 29.76% and 2.43%, 1.52% and 1.78%, respectively. These results suggest that the activation of in vitro matured oocytes by 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP for 4 h in mCR[2]aa is most favorable for parthenogenetic caprine embryos production