ABSTRACT
Evaluation of autogenous fibrinogen extracted from horse blood in fibrin glue and it's effects on horse's fibroblast and keratinocyte cells were preformed. Fibrinogen has been obtained by glycine precipitation technique from horse's blood. Fibroblast cells were separated by explant culture method from neck skin and keratinocytes were separated by enzyme digestion from lib samples. Autologous fibrinogen, horse`s fibroblast and keratinocyte cells separately mixed with bovine thrombin, calcium gluconate and tranexamic acid. This mixture was uniformly spread on cell culture plate and evaluated for six days. After one day of culture the thickness of fibrin glue were observed by invert microscope, fibroblast cells in elongated shape were observed in the fibrin glue in all thickness and keratinocyte cells were flattened and adherent on surface of plate. Both of them proliferated after 3 days. Fibrinogen that was obtained from horse blood by glycin precipitation technique can support horse fibroblasts as a scaffold and support horse keratinocyte proliferation in vitro
Subject(s)
Animals , Fibroblasts , Keratinocytes , HorsesABSTRACT
In this study, in vitro growth characteristics of fibroblasts of metacarpal region of horses and Caspian miniature horses were evaluated. Under general anesthesia and aseptic condition, A full thickness skin incision was created on the lateral aspect of mid-third of left metacarpal region of 4 mixed thoroughbred horses and 4 caspian miniature horses and 3 grams of subcutaneous tissue was harvested and placed in culture medium [RPMI- 1640] in an incubator at 37[degree sign] C [5%CO2]. After growth of fibroblasts, count of cells was performed for 8 days and growth rates and percentage viability of fibroblasts were recorded. There were no significant differences in the growth rates and viability rate of fibroblasts between horses and Caspian horses. It was therefore concluded that due to Genetic similarity between horses and Caspian horses, growth characteristics of fibroblasts in these two groups are the same, but Further In vivo research is needed to identify the wound healing pattern in Caspian horses in compare with horses
ABSTRACT
Assessment of Low Level Laser [LLL] effectson muscular repair and capability following sciatic nerveinjury as animal model.Experimental study.Ten male and female 30 +/- 6 month-old mixed breed dogs between 27 +/- 6 kg body-weight.Animals were subsequently divided into two groups of [control and experimental] of 5 dogs each. Then anesthesia was induced. Skin was incised and the muscular dissection performed. The right sciatic nerve was exposed in all dogs and crushed in full thickness using artery forceps for 5 seconds. The experimental group wassubjected to LLL regimen, daily ten minutes for twoweeks. The electromyography [EMG] was recorded fromsemimembranosus [S1], andsemitendinosus [S2], Extensors [Ext], and Flexors [Flex] muscles before, aftercrush, 14, 28 and 45 post-operative days.Variance analysis by ANOVA andTukey's test.EMG recording showed significant differences inmuscle forced in SI and S2 in experimental group bycomparison with control one.The evaluation of LLL showed tobe helpful to reduce muscle atrophy