[ role of Lactobacillus bioencapsulated Daphnia magna on the growth and feeding efficiency in Persian sturgeon [Acipenser persicus] larvae]

The use of probiotic bacteria has been suggested as an important strategy to accomplish reproducible outputs through biocontrol in cultivation systems for marine fish larvae and crustaceans. These bacteria have beneficial effects on fish larvae. This study was done to determine the effect of probiotic lactobacilli on the growth and feeding performance of Persian Sturgeon [Acipenser persicus] larvae via bioencapsulation with Daphnia magna. Daphnia magna was enriched by probiotic lactobacillus for 8 hours in three levels of 4.30, 4.60 and 4.78 log of colonies forming unit per milliliter in suspension of broth, and fed by A. persicus larvae in experimental treatments [treatments of T1, T2 and T[3]]. The Persian sturgeon larvae were fed on D. magnaon the base of 30 percent of their body weight for 30 days. The control treatment was fed on unbioencapsulated D. magna. At the termination of the experiment, the whole body samples of the fish were analyzed according to the AOAC procedures [1990]. The probiotic lactobacillus significantly promoted the body weight, levels of crude protein and carcass dry matter of larvae in experimental treatments in comparison with control treatment [p<0.05]. But in the treatment T[3], the crude lipid and crude energy were significantly decreased [p<0.05]. The maximum level of average crude protein in T[3] [70.27 +/- 0.44%] and its minimum in control [68.51 +/- 0.34%] were obtained. This study indicated that the blend of Lactobacillus had an effect on the promotion of some of the growth and feeding parameters in Persian sturgeon larvae

Distribution of hepatitis C virus genotypes in iranian patients with congenital bleeding disorders

Chronic hepatitis C virus [HCV] infection is the major cause of liver disease related morbidity and mortality in hemophilic patients who needs regular blood product administration. Although genotype of infecting HCV is one of the prime predictors of response to antiviral therapy however, its distribution in hemophilic patients is still unclear and just few studies with low sample sizes have investigated this issue. Therefore, in this study, we aimed to identify this distribution in 367 Iranian hemophilic patients. Blood samples were received from 367 hemophilic patients with chronic hepatitis C detected during a nationwide screening program who referred to our center for therapeutic measures. HCV RNA viral load was detected using Amplicor test [Version 2]. Genotyping was performed by genotype specific primers. HCV genotype distribution was 1a in 58%, 3a in 18.5%, 1b in 14.7%, 4 in 1.1%, 2 in 0.8% and mixed in 6.2% and finally 0.5% of isolates were non-typable. Serum liver enzymes were not associated with HCV viral load and genotypes. Patients with severe bleeding tendency had significantly lower serum liver enzymes than those with a mild bleeding tendency. Genotype 1a followed by 3a and 1b were the most frequently detected HCV genotypes in Iranian hemophilic patients and there was no association between splenomegaly and viral markers and liver enzymes in these patients

comparison on histopathological effects of myrtle extract and silver sulfadiazine 1% on healing of second degree burn wound in rats

Myrtle [Myrtus Communis] is an indigenous plant of northern parts of Iran and has been traditionally used for its healing effect on burn wounds. To compare the histopathological effect of Myrtle extract and 1% silver sulfadiazine on healing of second degree burn wound in rats. This was an experimental study performed on 100 adult male rats. The rats were divided into 4 groups [25 in each group]. Following nesdonal anaesthesia, a second degree burn wound of 4 cm2 was made on back of each rat. Methanolic extract of Myrtle; ointment base; 1% silver sulfadiazine and normal saline [control group] were used as treatment protocols for burn wounds. Revascularization, number of neutrophils and fibroblasts were parameters studied histopathologically. Data were analyzed using Chi-square, Kruskal-Wallis, One way ANOVA and Tukey's HSD tests. Histopathological study was indicative of an increase of 9.87 +/- 1.48 in revascularization [p<0.001] in Myrtle extract group which was greater than those in other. There was also a higher number of fibroblasts [23.62 +/- 2.2] [p<0.001] in this group. Silver sulfadiazine [1%] was only showed to have increased the number of neutrophils [p<0.001] in epidermis of injured areas of rats. Extract of Myrtus Communis was found to have more healing potential on second-degree burn wound. Faster healing process, less complications and easy availability of Myrtle are among the reasons suggesting more clinical trails using this preparation..

[Pregnancy outcome in premature delivery of between 34-37 weeks and the effects of corticosteroid on it]

Respiratory Distress Syndrome [RDS] is one of the important causes of newborn mortality. The aim of this study is to determine the pregnancy outcome and corticosteroid effects for prevention prophylaxis in pregnant women with premature labour between 34-37 weeks gestational age. This semi experimental single blind clinical trial Study was performed on hundred 34-37 weeks pregnant women who were referred with labour pain or rupture of membrane. These women divided in two almost similar case and control groups. The control group received 5mg dexamethasone 6 hours to delivery time [maximum 4 doses]. Data was entered to SPSS and analyzed by Chi-Square and T-Test. There wasn't any significant difference between administration of dexamethasone and incidence of RDS in 34-37 weeks newborn [p=0.6]. The Frequency of RDS was similar in two groups [in all women: 4%] the mean weight of newborns with RDS diagnosis was 2675 +/- 263.76% of women had vaginal delivery. The mean weight of newborn was 2672 gr and mean gestational age was 35.4 weeks]. The frequency of RDS in newborn with 34-37 weeks gestational age was 4% and administration of one or more dexamethasone had no effect on reduction it