ABSTRACT
To determine the effects of herbal drugs in Hypercholesterolemia, Experimental Study, This study was conducted in the Department of Pharmacology, University of Karachi from Jan. 2007 to Dec, 2008. In the present study experiments regarding lipid profile were performed on rabbits of either sex, the calculated dose was administered for 30 days and 60 days [High dose and low dose group], Alfagin possess wide therapeutic range and is comparatively safe, animals of neither group showed no gross toxicity, No death occurred in control and test animals, Alfagin has been shown having, lowering capability of cholesterol. It has been concluded that Alfagin is beneficial in hypercholesterolemia
ABSTRACT
Entoban is a well balanced composition of medicinal herbs which includes Holarrhena antidysenterica, Berberis aristata, Aegle marmelos, Quercus infectoria, Butea Frondosa and Myrtus Communis. It is used in amoebic dysentery. The study was carried out on 27 healthy white rabbits of either sex weighing from 1800 to 2000 grams. All animals were equally divided into three groups one served as control, the other two received normal and high doses of entoban syrup. Before administration of the drug animals were acclimatized to housing conditions for 15 days. Entoban was administered in normal dose 1.14 mg / kg and 2.29 mg / kg high dose for a period of 60 days. The control group received normal saline equivalent to the volume of respective doses according to their body weight. Blood samples of about 7cc were collected from the animals through cardiac puncture various biochemical tests were done to consider cardiac, renal and hepatic parameters were done. Cardiac, renal and hepatic parameters were found to be in normal range with high dose. It de-termines that entoban is a safe drug. Therefore cardiac, renal, and hepatic toxicity has been ruled out. Entoban can be safely administered for amoebic dysentery because it does not affect the heart, liver and kidneys
ABSTRACT
Doxorubicin an anthracycline antibiotic and antitumour agent is one of the highly effective antineoplastic drug for the treatment of several different human cancers. The clinical use of this drug is limited by its cardiotoxicity including dose related arrhythmias and often irreversible add from discussion cardiomyopathy. The inotropic and chronotropic activity of different doses of doxorubicin was first evaluated and after determining the fixed effective dose of doxorubicin, the beta receptor activity of doxorubicin was evaluated. The effect of propranolol on heart in presence of doxorubicin was evaluated. Thirty male rabbits weighing 1000 - 1500 grams were used. Drugs used were Doxorubicin and Propranolol. The actions of drugs were studied on the isolated heart of these animals. Krebs ringer buffered physiological solution was prepared and used. To perform the experiment complete set of Lagendroff's apparatus was used. Recording was done on graph paper. Other accessories used were isometric transducer, thermostat oxygen, one ml graduated insulin syringe [to inject the test samples]. Pate of contraction of heart and amplitude of contraction was measured [in centimeters] from baseline. Results were calculated statistically on S.P.S.S. We have evaluated the physiological interactions of Doxourbicin with Adrenaline [agonist] in the first part of the study and in this study the interaction with antagonist [Propranolol] has been studied to evaluate the effects leading to up or down regulation of b receptors
ABSTRACT
Griseofulvin is widely used for the treatment of fungal infections in the local population, but it is reported to cause a number of side effects in sensitive people. The drug has not undergone any worthwhile clinical studies for the side effects in the local population. In view of the possibilities of differences in drug effects and dispositions in the ethnic groups, it was found imperative to investigate the effects of griseofulvin administration on the liver functions, blood picture and for the manifestation of any untoward side effects in the local population being treated with the drug. After 45 days of the drug treatment at a dose level of 500 mg/day, parameters of liver functions and the blood picture were found to be within the normal limits. Some transient side effects like headache in 10.8%; dizziness in 1.6%; nausea, vomiting and diarrhoea in 3.2%; flatulence, heartburn and dry mouth in 2.2% urticaria in 0.54% and pruritus in 0.54% of the patients was observed. These effects disappeared when the drug was withdrawn. No significant increase was found in the levels of serum bilirubin, serum glutamatepyruvate - transaminase [SGPT] and serum alkaline phosphatase. The values were found to in the normal range. Blood picture also showed no abnormality or shift in the cellular components. It was, therefore, concluded that griseofulvin can safely be used by the local population for treatment of fungal infections at the prescribed dose level
Subject(s)
Liver/drug effects , Liver Function Tests/methodsABSTRACT
Studies were performed on albino rats to investigate any untoward effects of griseofulvin on their liver function. For this purpose, animals weighing between 200 - 250g were selected. The animals were divided into a control and four test groups containing ten animals in each group. Griseofulvin was administered orally to test groups A, B, C and D at a does level of 20mg/kg day, for 2,4, 6 and 8 weeks respectively. At the end of the study period, the animals were sacrificed and their blood was collected by direct cardiac puncture. Liver function tests were carried out by determining the levels of alkaline phosphatase and glutamate - pyruvate transaminase in the serum of the control and the test groups. No significant difference was found in the levels of the two enzymes investigated, when the values in the test groups were compared with those of the control group, showing that the drug did not manifest any untoward effects at the dose level used in the present study which is slightly more than the dose level effective therapeutically. It was, therefore, concluded that Griseofulvin has no hepatotoxic effects in albino rats at a dose level of 20mg/kg/day