Synthesis and antitumor activity of some hydrazinoacridine hydrazinoacridonanil and 9-oxo-9, 10-dihydroacridin-4-carboxazide derivatives

Six series of acridine derivatives of anticipated antitumor activity have been designed and synthesized. The first comprised 9-[4 sulfamoylphenyl] hydrazinoacridine. The second consisted of 9-[4-sulfamoylphenyl] hydrazinoacridonanil. The third included N- substituted 9-[4 sulfamoylphenyl] hydrazinoacridine-4-yl [N-[4-sulfamoylphenyl]] carboxamide. While the fourth and the fifth included N substituted 9-[4-sulfamoylphenyl]] hydrazinoacridin-4-yl[N- sulfamoylphenyl]] carboxazide derivatives; and the sixth included 9-oxo-9, 10-dihydroacridin-4-yl [N-[4-sulfamoylphenyl]] carboxazide derivatives. The rationale behind the synthesis of these compounds, their methods of synthesis as well as the antitumor activities have been presented

1H-NMR spectroscopic quantitative determination of clobazam

The 1H-NMR spectrum of clobazam showed a sharp singlet at 3.49 ppm assigned for the N-CH3, which was chosen for the quantitative measurements. The method involves comparing the integral of this signal to that of a sharp singlet at sigma 6.41 ppm assigned for maleic acid, used as internal standard for comparison. A standard curve was plotted the integration ratio Ic/Im and the amount of clobazam in mg. Good results were obtained on application of this method on authentic sample of clobazam. The mean percentage recovery is 100.12 +/- 1.133. Good recoveries were also obtained on application of this method on tablet form [Frisium] using standard addition technique. The mean percentage recoveries are 100.45 +/- 1.899 and 100.45 +/- 0.659. The results were compared with the reported spectrophotometric method. According to the variance ratio test [F test] and Student's t-test, there is no significant difference between the two methods, with respect to the precision and accuracy