ABSTRACT
Released dopamine from dopaminergic neurons in the substantia nigra pars compacta affects dentate gyrus [DG] neurogenesis in the hippocampus [HPC]. Damage to dopaminergic neurons in Parkinson's disease [PD] causes decreased neurogenesis in DG which results in memory impairment. This was an experimental laboratory study. We assessed the effect of intravenous transplantation of adipose-derived stem cells [ADSCs] on hippocampal neurogenesis after inducing injury by 6-OHDA [memory disability model of PD].We performed bilateral injections of 6-OHDA into substantia nigra [SNc] of male Wistar rats. First group of the rats received bilateral injections of 6-OHDA [6 micro g] dissolved in 2 micro l saline. Second group received saline injections instead of neurotoxin [sham group]. In the third group we transplanted the 3[rd] passage of ADSC cells which had been assessed for CD90 immunostaining [1×10[6] in 500 microl medium], via tail vein. The 4[th] group included injured rats which received an injection of the fluid of the culture media [500 micro l] through tail vein. After treatment, rats were sacrificed. The brains of the rats were removed, fixed with 4% paraformaldehyde, dehydrated, embedded in paraffin and cut into 10 micro m thick slices. We stained the sections with cresyl violet and determined the density of neurons in DG, CA1, CA3. Statistical analysis was performed by one-way ANOVA and Tukey test. P
ABSTRACT
The main goal was to address the prevalence of enteric protozoan parasites in rural areas of Bandar-Abbas, southern Iran and to compare the results with the only conducted study in 1978. This descriptive study was performed from 2009 through 2010 on the 565 fecal samples. Formalin-ether concentration technique was performed and the analysis was carried out using Chi-square test in SPSS software version 13.5. Finally, the comparison of our results with the only previous study which was accomplished by Sheiban and Rezaeian in 1978 was done. The overall prevalence of the protozoan parasites was 48.8%. However, the prevalence of pathogen parasites was 23%. Previous research in 1978 showed 80.4% infectivity. The most protozoan parasites were Blastocystis hominis [25.53%], Giardia lamblia [17.2%] and Entamoeba coli [15.95%]. Previous study in 1978 found Entamoeba coli as the most common protozoa. Our finding revealed that the rate of single infectivity was much higher compared to previous research. The most frequency of infection was in children. The remarkable decrease of protozoan parasites is mainly due to progress in health care in the villages; however more effort should be done with the goal of eradicating infectious agents