ABSTRACT
To see the accuracy and role of fine needle aspiration cytology in children with peripheral lym-phadenopathy in our setup. Descriptive study. Department of Pathology Peoples Medical College Nawabshah. January 2002 to December 2004. A total number of 158 children were included in the study having age upto 12 years with history of peripheral lymphadenopathy. Fine needle aspiration cytology was performed with 22 gauge needle attached with 10 ml syringe and without using any local anesthetic. Confirmation of the diagnosis by subsequent surgical biopsy or by a clinical follow up period for at least six months was mandatory. 179 aspirates were obtained from 158 patients. The cytological diagnosis were; 78 [43.6%] reactive, 43 [24.0%] tuberculous lymphadenitis, 27 [15.1%]suppurative inflammation, 15 [8.3%] non Hodgkin's lymphoma, 05 [2.8%] Hodgkin's lymphoma, 03 [1.7%] leukemic infiltration, 02 [1.1%] cytological diagnosis uncertain, 06 [3.4%] material inadequate. 06 cytological diagnosis were false negative and one was false positive. The overall accuracy was 96%. Fine needle aspiration cytology is an initial important diagnostic tool in children having peripheral lymphadenopathy, especially in those rural areas where the surgical facilities are not available. It is a safe, rapid simple and accurate with low morbidity -and only the doubtful cases may go for surgical biopsy
ABSTRACT
To asses the frequency of HER-2/neu Oncoprotein over expression in invasive ductal breast cancer and correlate it with various prognostic parameters. Cross-sectional study. Department of pathology Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. Seventy two [72] formalin fixed paraffin embedded blocks of diagnosed invasive ductal carcinoma were retrieved and 5 p.m thick sections were cut and stained with H and E for the review of diagnosis and grading. The immunohistochemical staining was done on 4p.m thick sections by using Polyclonal Rabbit anti HER-2 ZYMED USA, and ZYMED 2nd generation LAB-SA immunodetection system, to see the HERVneu over expression. Section containing > 50% of tumor cells exhibiting intense circumferential cell membrane stainingscored as positive. Thirty one [31%] positive over expression was observed. A statistically significant result [P<.05] was found between HER-2/neu over-expression and lymph node status and size of tumor. Oncoprotein HER-2 / neu over expression is directly related to the lymph node status and size of the tumor. These patients may benefit from a more aggressive therapy like Herceptin and can also be used as a prognostic marker in the follow up of these patients
ABSTRACT
This study was designed to determine the frequency of HER-2/neu overexpression, in invasive ductal carcinoma of breast and correlate it with various standard prognostic parameters. This study was performed on formalin fixed paraf n embedded blocks of 72 diagnosed cases of invasive ductal carcinoma of breast. The blocks were retrieved and the staining was done to see the HER-2/neu overexpression. Sections containing > 50% of tumour cells showing intense circumferential cell membrane staining scored as positive. The HER-2/neu overexpression was seen in 31% of cases. A significant relationship [P<0.05] was found between HER-2/neu overexpression and lymphnode status and size of the tumour. The overexpressivity of HER-2/neu oncoprotein suggests that Herceptin can be used as therapeutic measure on these cases