Rate of Listeria abortion in Tehran

The rate of listeria abortion in Tehran was investigated, Abortion samples [200] were cold enriched at 4°C and subcultured on selective culture media containing acriflavin, nalidixic acid and potassium thiocyanate. Sera of patients were tested serologically [IF method] for screening, and results were confirmed by culturing the positive samples. Antibody against L. monocytogenes was obtained in 70.7% of sera but the bacteria was isolated from five samples only

Determination of dominant serovars of Listeria monocytogenes

Serovars of Listeria monocytogenes were determined. Sera of aborted samples [200] were collected from different hospitals in Tehran and were tested serologically by immu no flu ore scent antibody methods [IF tests]. 137 positive sera were identified. Positive sera were tested against 12 serovars of Listeria monocytogenes separately. Titers of antibody in patients' sera for all serovars were determined. The results showed that the dominant serovars of L. monocytogenes which caused listeriosis in the samples were 4b, la, 2 and 3, None of the sera had antibodies against serovars 4a, 6a or 6b. Some of the sera which had high titers of antibody against dominant serovars [4b, 1 a, 2 and 3], showed a faint result with serovars 4d, 4e,5 and 7