Chemical composition of essential oil from ripe fruit of Schinus terebinthifolius Raddi and evaluation of its activity against wild strains of hospital origin

The essential oil (EO) composition of ripe fruit of S. terebinthifolius Raddi was analyzed by GC-MS. The oil extraction yielded 6.54 ± 1.06% (w/w). Seventeen compounds were identified, accounting for 91.15% of the total oil, where monoterpenes constituted the main chemical class (85.81%), followed by sesquiterpenes (5.34%). The major monoterpene identified was δ-3-carene (30.37%), followed by limonene (17.44%), α-phellandrene (12.60%) and α-pinene (12.59%). Trans-caryophyllene (1.77%) was the major sesquiterpene identified. The antibacterial activity of the essential oil was evaluated against wild strains of hospital origin (Escherichia coli, Pseudomonas sp., Klebsiella oxytoca, Corynebacterium sp., Staphylococcus aureus, Enterobacter sp., Enterobacter agglomerans, Bacillus sp., Nocardia sp. and Streptococcus group D). The essential oil of the ripe fruit of S. terebinthifolius Raddi has shown to be active against all tested wild strains, with minimum inhibitory concentration ranging from 3.55 μg/mL to 56.86 μg/mL. However, it has revealed some differences in susceptibility: the general, Gram-positive species showed greater sensitivity to the action of EO, which is probably due to the lower structural complexity of their cell walls.

The male frog (Rana vigittera) biological pregnancy test as compared to the immunologic pregnancy test

Frog tests were done using solutions with different known concentrations of human chronic gonadotropin per cc: 3:4:5:6:I.U./cc. solutions. Positive results in percentage were 20%:42%:61% and 80% respectively. Solutions lower than 3 I.U./cc were not used because earlier studies done by the Pasay-Paranaque Medical Society had shown negative results with solutions of 1 I.U./cc. and 2 I.U./cc. of HCG. On the other hand, immunologic haemogglutination inhibition tests gave positive reaction or pregnancy test with solutions having as low as 1/2 I.U. of HCG/cc. (Summary)

Treatment of iron deficiency anemia by total dose infusion (TDI) of iron dextran

Twenty-eight patients received intravenous iron-dextran (TDI) for correction of iron deficiency anemia. One experienced a systemic reaction but recovered without untoward effects, another developed uterine contraction and both of this case were not included in this studiesThe rise in hemoglobin and hematocrit were noted after two weeks and was rapid over a period of six weeks. No delayed reactions were seen in all the casesWe believe that, although not without danger, the use of Iron-Dextran Infusion (TDI) by the method and techniques described should find a useful place in correction of severe iron deficiency anemia. (Summary)